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Diss Factsheets
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EC number: 247-668-1 | CAS number: 26402-26-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- toxicity control from ready biodegradability study
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data based on the inhibition control of a ready biodegradability guideline study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 1992
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt Baden-Württemberg, Germany
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 164 mL of the test item stock solution with 48.1 mg/L and a pH of 7.5 and 21.0 mg of the reference substance were added into the toxicity control vessel (corresponding to a concentration of 200 mg/L* COD). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal wastewater treatment plant AZV Breisgauer Bucht (predominantly domestic wastewater), sampling date of activated sludge: 26 July 2022,
- Preparation of inoculum for exposure: The activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge. The sludge was aerated at ambient temperature until use.
- Concentration of sludge: 30 mg d.s./L
- Dry solids concentration: 4.27 g/L (drying at 105°C for 5 hours)
- Water filtered: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Remarks on exposure duration:
- Overall exposure was 28 d but the inhibition effect is evaluated after 14 d.
- Test temperature:
- 21.3 – 21.5°C
- pH:
- 7.5
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: according to the guideline 301 F
- Solubilising agent: none
- Test temperature: 21.3 – 21.5°C throughout the whole study and therefore within the required range of 22 ± 2°C
- pH: 7.5 (at the beginning and termination of test)
- pH adjusted: The pH of the mineral medium was 7.7 and was therefore adjusted to 7.5 with HCl. After preparation of test solutions, no pH adjustment was needed (pH: 7.5)
- Aeration of dilution water: yes, at least half an hour
- Susspended solids concentration: 30 mg d.s./L
TEST SYSTEM
- Culturing apparatus: OxiTop® (WTW / Xylem)
- Method used to create aerobic conditions: inoculum was aerated until use (see details on inoculum and medium/test solutions were prepared under oxic conditions)
- Measuring equipment: Sensomat (AQUALYTIC®)
- Test performed in closed vessels: yes
- Test performed in open system: no
- Details of trap for CO2 if used: bottles were equiped with rubber quiver inserts for NaOH pellets, which absorb the carbon dioxide produced.
SAMPLING
- Sampling frequency and method: Every 112 minutes, the current pressure was measured and stored by each measuring head.
- Sterility check if applicable: not applicable
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates (only inoculum)
- Reference substance: 3 replicates (concentration corresponding to 99.8 mg COD/L)
- Toxicity control: 1 replicate (48.1 mg/L test item and 21 mg reference substance corresponding to a concentration of 200 mg/L COD) - Reference substance (positive control):
- yes
- Remarks:
- acetic acid, sodium salt
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 48.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: BOD measurements and subsequent calculation of chemical oxygen demand
- Remarks on result:
- other: Result of toxicity control from ready biodegradability test
- Details on results:
- The degradation of the toxicity control after 14 days was >25%. Therefore, the test item had no inhibitory effect on the inoculum according to the criterion of the guideline OECD 301 F.
- Results with reference substance (positive control):
- The reference compound sodium acetate reached the pass level of 60% COD within 5 days.
Reference
Since more than 25% degradation occurred in the toxicity control after 14 d (48.9 on Day 4 and >80% on Day 12), the substance is with high probability not toxic to aquatic microorganisms. The test item concentration in the toxicity control of 48.1 mg/L can be used as NOEC value for the toxicity to aquatic microorganisms.
Description of key information
NOEC (14 d) ≥ 48.1 mg/L (nominal, toxicity control of OECD 301 F)
Key value for chemical safety assessment
Additional information
Since no studies on the toxicity to aquatic microorganisms are available a ready biodegradability study is used to derive a NOEC for the toxicity to aquatic microorganisms. If a compound degrades well in a ready biodegradability test or does not inhibit the degradation of a positive control at a certain concentration, this concentration can be used as a NOEC value. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
A substance can be assumed to be not inhibitory to aquatic microorganisms, if in the toxicity control of a ready biodegradation test, more than 25% degradation based on oxygen demand (BOD/ThOD) occurred within 14 days (OECD guideline 301). For Octanoic acid, monoester with glycerol (CAS 26402-26-6) a biodegradation test according to the OECD guideline 301 F is available (and GLP). The test includes a toxicity control, which contains 48.1 mg/L of Octanoic acid, monoester with glycerol and 21 mg/L of the reference material acetic acid, sodium salt (corresponding to 199 mg/L COD). The toxicity control attained 48.9% biodegradation on Day 4 and >80% on Day 12 of incubation. Hence, the substance is not considered toxic to aquatic microorganisms in the toxicity control and the test item concentration of 48.1 mg/L (nominal) can be used as NOEC.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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