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EC number: 241-543-5 | CAS number: 17572-97-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test item was stored in a closed vessel dry at room temperature (20 ± 5 °C).
- Vehicle:
- no
- Remarks:
- Due to the poor solubility of the test item, the test item was added directly into the test vessels.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant in D-67435 NW-Lachen-Speyerdorf, Im Altenschemel. Upon arrival in the test facility, the sludge was filtrated, washed with tap water 3 times and re-suspended in tap water. The activated sludge was aerated until usage in the test and fed daily with 50 mL synthetic sewage feed /L. In the first experiment (pre-test), the dry matter was determined as 2.92 g suspended solids/L, giving a concentration of 1.46 g suspended solids/L in the test. In the second experiment (main test), the dry matter of the inoculum was determined as 2.18 g suspended solids/L, giving a concentration of 1.09 g suspended solids/L in the test.
- Test type:
- not specified
- Water media type:
- other: tap water
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After 3 hours, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 minutes. The respiration rate was measured over a period of only 5 minutes. In the guideline, a period of 5 to 10 minutes or until the oxygen concentration falls below 2 mg/L is recommended. As the linearity of all regression linear curves (r2 of 0.99 or more) is given, the measurement period can be considered as sufficiently long.
- Hardness:
- 0.88 mmol/L
- Test temperature:
- 18.5 – 20.7 °C (first experiment (pre-test))
19.1 – 21.4 °C (second experiment (main test)) - pH:
- The pH of the nutrient solutions was 7.0.
The pH of the water used to prepare the solutions was 8.01. - Conductivity:
- 210 µS/cm
- Nominal and measured concentrations:
- In the first experiment (pre-test) the test item was tested using 4 concentrations ranging from 1000 to 1 mg/L (1000, 100, 10 and 1 mg/L) nominal concentration.
In the second experiment (main test) the test item was tested using 5 concentrations ranging from 1000 to 10 mg/L (1000, 320, 100, 32 and 10 mg/L) nominal concentration. - Details on test conditions:
- In the blank control vessels, 16 mL nutrient solution (synthetic sewage) was mixed with 234 mL water. The positive control vessels and the treatments were prepared by putting the appropriate amount of positive control solution or test item solution into the respective test vessel, adding 16 mL nutrient solution and water to reach a total volume of 250 mL. Then, 250 mL inoculum was added in 5 minutes intervals and the mixtures were aerated.
After 3 hours, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 minutes . The following vessels were measured likewise in 5 minutes intervals. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (CAS-No. 591-35-5) was used as positive control. A stock solution in deionised water containing 501 mg/L of 3,5-Dichlorophenol was freshly prepared for each experiment.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: confidence interval 350 - 860 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 190 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: confidence interval 100 - 270 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- For evaluation of the results the nominal concentrations were used because the difference between real concentration and nominal concentration can be stated as not significant.
For the calculation of the EC10 and EC50, the percentage inhibition was plotted versus concentration in a Gauß-logarithmic diagram. EC10 and EC50 were determined from the x values of the regression line at y = 10% and y = 50%.
The coefficient of variation of oxygen uptake rate in blank control replicates was not more than 30% at the end of the test.
The blank controls oxygen uptake rate was more than 20 mg oxygen per gram of activated sludge (dry weight of suspended solids) in 1 hour. - Results with reference substance (positive control):
- Concentrations of the positive control were calculated using the concentration of the stock solution and the dilution factor.
- Reported statistics and error estimates:
- For the treatments with the test item concentrations 1000 and 320 mg/L, it was tested whether the differences between treatment and blank control were significant. For this determination, the values of the O2 consumption were used. The difference between treatment 320 mg/L and the blank control was considered as not significant as the calculated t-value laid below the tabulated t-value and O2 consumption in the treatment was higher than in the blank control. Therefore, the concentration 320 mg/L is stated as NOEC.
- Validity criteria fulfilled:
- yes
- Remarks:
- No observations were made which might cause doubts concerning the validity of the study outcome.
- Conclusions:
- The following results for the test item CDTA HHQ were determined:
3h NOEC = 320 mg/L
3h EC10 = 190 mg/L (confidence interval 100 - 270 mg/L)
3h EC50 = 500 mg/L (confidence interval 350 - 860 mg/L)
Note: According to the guideline, NOEC is determined by comparing the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower. - Executive summary:
Two experiments were performed.
In the first experiment (pre-test) the test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. For the treatment 1000 mg/L, 5 replicates were used; for the other treatments, 1 replicate each.
Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 2.92 g suspended solids/L, giving a concentration of 1.46 g suspended solids/L in the test.3,5-Dichloro-phenol was used as positive control.
Because significant inhibition was observed, an additional experiment had to be performed.
In the second experiment (main test) the test item was tested using 5 concentrations ranging from 1000 to 10 mg/L nominal concentration. For all treatments 5 replicates were used. The dry matter of the inoculum was determined as 2.18 g suspended solids/L, giving a concentration of 1.09 g suspended solids/L in the test.
All validity criteria were met. For the estimation of the EC50 of the positive control, the fits showed good statistical correspondence of the data with the dose-response-equation. The positive control gave an EC50 of 8.8 mg/L (first experiment pre-test) and 8.4 mg/L (second experiment main test) which lie within the recommended range of 2 – 25 mg/L stated in the OECD guideline. The coefficient of variation of oxygen uptake rate in control replicates was below 30% at the end of the test. The oxygen uptake rate of the blank controls was above 20 mg O2per gram activated sludge in 1 hour.
For evaluation of the results the nominal concentrations were used because the difference between real concentration and nominal concentration can be stated as not significant.
Because no inhibition was observed in the 3 lowest concentrated treatments, the 2 lowest concentrated treatments were not used for evaluation to receive a linear regression.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- January 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- It is reasonable that the toxicity to microorganisms of tripotassium hydrogen ethylenediaminetetraacetate is caused by the ammonium groups of the substance. In a read-across approach, a guideline study on CDTA is used to evaluated the toxicity as these substance has the same moiety. Furthermore, with 4 ammonium groups (in CDTA) compared to only 2 (in tripotassium hydrogen ethylenediaminetetraacetate) it can be assume that the toxicity of the latter is not higher than the one of the first. Accordingly, the toxicity might be overestimated but not underestimated.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test item was stored in a closed vessel dry at room temperature (20 ± 5 °C).
- Vehicle:
- no
- Remarks:
- Due to the poor solubility of the test item, the test item was added directly into the test vessels.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant in D-67435 NW-Lachen-Speyerdorf, Im Altenschemel. Upon arrival in the test facility, the sludge was filtrated, washed with tap water 3 times and re-suspended in tap water. The activated sludge was aerated until usage in the test and fed daily with 50 mL synthetic sewage feed /L. In the first experiment (pre-test), the dry matter was determined as 2.92 g suspended solids/L, giving a concentration of 1.46 g suspended solids/L in the test. In the second experiment (main test), the dry matter of the inoculum was determined as 2.18 g suspended solids/L, giving a concentration of 1.09 g suspended solids/L in the test.
- Test type:
- not specified
- Water media type:
- other: tap water
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After 3 hours, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 minutes. The respiration rate was measured over a period of only 5 minutes. In the guideline, a period of 5 to 10 minutes or until the oxygen concentration falls below 2 mg/L is recommended. As the linearity of all regression linear curves (r2 of 0.99 or more) is given, the measurement period can be considered as sufficiently long.
- Hardness:
- 0.88 mmol/L
- Test temperature:
- 18.5 – 20.7 °C (first experiment (pre-test))
19.1 – 21.4 °C (second experiment (main test)) - pH:
- The pH of the nutrient solutions was 7.0.
The pH of the water used to prepare the solutions was 8.01. - Conductivity:
- 210 µS/cm
- Nominal and measured concentrations:
- In the first experiment (pre-test) the test item was tested using 4 concentrations ranging from 1000 to 1 mg/L (1000, 100, 10 and 1 mg/L) nominal concentration.
In the second experiment (main test) the test item was tested using 5 concentrations ranging from 1000 to 10 mg/L (1000, 320, 100, 32 and 10 mg/L) nominal concentration. - Details on test conditions:
- In the blank control vessels, 16 mL nutrient solution (synthetic sewage) was mixed with 234 mL water. The positive control vessels and the treatments were prepared by putting the appropriate amount of positive control solution or test item solution into the respective test vessel, adding 16 mL nutrient solution and water to reach a total volume of 250 mL. Then, 250 mL inoculum was added in 5 minutes intervals and the mixtures were aerated.
After 3 hours, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 minutes . The following vessels were measured likewise in 5 minutes intervals. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (CAS-No. 591-35-5) was used as positive control. A stock solution in deionised water containing 501 mg/L of 3,5-Dichlorophenol was freshly prepared for each experiment.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: confidence interval 350 - 860 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 190 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: confidence interval 100 - 270 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- For evaluation of the results the nominal concentrations were used because the difference between real concentration and nominal concentration can be stated as not significant.
For the calculation of the EC10 and EC50, the percentage inhibition was plotted versus concentration in a Gauß-logarithmic diagram. EC10 and EC50 were determined from the x values of the regression line at y = 10% and y = 50%.
The coefficient of variation of oxygen uptake rate in blank control replicates was not more than 30% at the end of the test.
The blank controls oxygen uptake rate was more than 20 mg oxygen per gram of activated sludge (dry weight of suspended solids) in 1 hour. - Results with reference substance (positive control):
- Concentrations of the positive control were calculated using the concentration of the stock solution and the dilution factor.
- Reported statistics and error estimates:
- For the treatments with the test item concentrations 1000 and 320 mg/L, it was tested whether the differences between treatment and blank control were significant. For this determination, the values of the O2 consumption were used. The difference between treatment 320 mg/L and the blank control was considered as not significant as the calculated t-value laid below the tabulated t-value and O2 consumption in the treatment was higher than in the blank control. Therefore, the concentration 320 mg/L is stated as NOEC.
- Validity criteria fulfilled:
- yes
- Remarks:
- No observations were made which might cause doubts concerning the validity of the study outcome.
- Conclusions:
- The following results for the test item CDTA were determined:
3h NOEC = 320 mg/L
3h EC10 = 190 mg/L (confidence interval 100 - 270 mg/L)
3h EC50 = 500 mg/L (confidence interval 350 - 860 mg/L)
As justified above, it is reasonable to assume the the values for tripotassium hydrogen ethylenediaminetetraacetate are the same or even higher.
Note: According to the guideline, NOEC is determined by comparing the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower. - Executive summary:
Two experiments were performed.
In the first experiment (pre-test) the test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. For the treatment 1000 mg/L, 5 replicates were used; for the other treatments, 1 replicate each.
Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 2.92 g suspended solids/L, giving a concentration of 1.46 g suspended solids/L in the test.3,5-Dichloro-phenol was used as positive control.
Because significant inhibition was observed, an additional experiment had to be performed.
In the second experiment (main test) the test item was tested using 5 concentrations ranging from 1000 to 10 mg/L nominal concentration. For all treatments 5 replicates were used. The dry matter of the inoculum was determined as 2.18 g suspended solids/L, giving a concentration of 1.09 g suspended solids/L in the test.
All validity criteria were met. For the estimation of the EC50 of the positive control, the fits showed good statistical correspondence of the data with the dose-response-equation. The positive control gave an EC50 of 8.8 mg/L (first experiment pre-test) and 8.4 mg/L (second experiment main test) which lie within the recommended range of 2 – 25 mg/L stated in the OECD guideline. The coefficient of variation of oxygen uptake rate in control replicates was below 30% at the end of the test. The oxygen uptake rate of the blank controls was above 20 mg O2per gram activated sludge in 1 hour.
For evaluation of the results the nominal concentrations were used because the difference between real concentration and nominal concentration can be stated as not significant.
Because no inhibition was observed in the 3 lowest concentrated treatments, the 2 lowest concentrated treatments were not used for evaluation to receive a linear regression.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Tripotassium hydrogen ethylenediaminetetraacetate dissociates into potassium cations and anions of edetic acid.
The first are known to be non-toxic as potassium is an essential element for all species.
The EU risk assessment reports collected data of toxicity studies on microorganisms for edetic acid and several salts (Na2H2EDTA, Na4EDTA).
Those substance dissociate into non-toxic cations and the same edetic acid anions as tripotassium hydrogen ethylenediaminetetraacetate.
As the relevant species are exactly the same, also the toxicity is the same and thus a read-across approach is justified. - Qualifier:
- no guideline available
- Principles of method if other than guideline:
- These tests are not well-documented. It is unclear whether the test organisms are sufficiently supplied with trace nutrients. It cannot be excluded that, similar to algae, the effects were caused by nutrient deficiency.
Nevertheless the toxiciy might be overestimated but not underestimated.
The reliable respiratory test (according to OECD 209) confirms the low toxicity of edetic acid and it derivatives towards microorganisms. - GLP compliance:
- no
- Specific details on test material used for the study:
- Additionally Na2H2EDTA and Na4EDTA) were evaluated.
- Analytical monitoring:
- no
- Test organisms (species):
- other: several species were used. OECD 209 was performed with activated sludge.
- Key result
- Duration:
- 16 h
- Dose descriptor:
- NOEC
- Remarks:
- species: Pseudomonas putida
- Effect conc.:
- 105 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- referred to edetic acid
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 48 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- referred to edetic acid
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Due to the short test duration and limit test details this value was not considered as relevant.
- Key result
- Duration:
- 20 h
- Dose descriptor:
- NOEC
- Remarks:
- species: Uronema parduczi
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- referred to edetic acid
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Remarks:
- species: Chilomonas paramaecium
- Effect conc.:
- 510 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- referred to edetic acid
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Remarks:
- species: Entosiphon sulcatum
- Effect conc.:
- 28 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- referred to edetic acid
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Remarks:
- activated sludge according to OECD 209
- Effect conc.:
- >= 500 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- referred to edetic acid
- Details on results:
- The TGK value (toxic limit concentration) is regarded as the NOEC value.
- Conclusions:
- In conclusion, the NOEC values reported by the risk assessment report are 13 mg/L or higher.
Referenceopen allclose all
Content of Test Vessels in the second experiment (main test):
Vessel No. |
Content |
Concentration (mg/L) nominal |
Nutrient Solution (mL) |
Tap Water (mL) |
Stock Solution added (mL) |
Concentration of Stock Solution (mg/L) |
Inoculum (mL) |
1 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
2 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
3 |
Positive Control |
5 |
16 |
229 |
5 |
501 |
250 |
4 |
Positive Control |
10 |
16 |
224 |
10 |
501 |
250 |
5 |
Positive Control |
20 |
16 |
214 |
20 |
501 |
250 |
6 |
Positive Control |
40 |
16 |
194 |
40 |
501 |
250 |
7 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
8 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
Vessel No. |
Content |
Concentration (mg/L) nominal |
Nutrient Solution (mL) |
Tap Water (mL) |
Amount added (mg) |
Calculated amount (mg) |
Inoculum (mL) |
9 |
Test Item |
1000 |
16 |
234 |
500.1 |
1000.2 |
250 |
10 |
Test Item |
1000 |
16 |
234 |
499.8 |
999.6 |
250 |
11 |
Test Item |
1000 |
16 |
234 |
500.3 |
1000.6 |
250 |
12 |
Test Item |
1000 |
16 |
234 |
500.6 |
1001.2 |
250 |
13 |
Test Item |
1000 |
16 |
234 |
499.8 |
999.6 |
250 |
14 |
Test Item |
320 |
16 |
234 |
159.6 |
319.2 |
250 |
15 |
Test Item |
320 |
16 |
234 |
160.4 |
320.8 |
250 |
16 |
Test Item |
320 |
16 |
234 |
160.9 |
321.8 |
250 |
17 |
Test Item |
320 |
16 |
234 |
159.8 |
319.6 |
250 |
18 |
Test Item |
320 |
16 |
234 |
159.6 |
319.2 |
250 |
19 |
Test Item |
100 |
16 |
234 |
50.2 |
100.4 |
250 |
20 |
Test Item |
100 |
16 |
234 |
50.1 |
100.2 |
250 |
21 |
Test Item |
100 |
16 |
234 |
50.2 |
100.4 |
250 |
22 |
Test Item |
100 |
16 |
234 |
50.3 |
100.6 |
250 |
23 |
Test Item |
100 |
16 |
234 |
49.7 |
99.4 |
250 |
Vessel No. |
Content |
Concentration (mg/L) nominal |
Nutrient Solution (mL) |
Tap Water (mL) |
Amount added (mg) |
Calculated amount (mg) |
Inoculum (mL) |
24 |
Test Item |
32 |
16 |
234 |
15.9 |
31.8 |
250 |
25 |
Test Item |
32 |
16 |
234 |
15.9 |
31.8 |
250 |
26 |
Test Item |
32 |
16 |
234 |
16.1 |
32.2 |
250 |
27 |
Test Item |
32 |
16 |
234 |
16.1 |
32.2 |
250 |
28 |
Test Item |
32 |
16 |
234 |
16.1 |
32.2 |
250 |
29 |
Test Item |
10 |
16 |
234 |
4.9 |
9.8 |
250 |
30 |
Test Item |
10 |
16 |
234 |
5.1 |
10.2 |
250 |
31 |
Test Item |
10 |
16 |
234 |
4.9 |
9.8 |
250 |
32 |
Test Item |
10 |
16 |
234 |
5.1 |
10.2 |
250 |
33 |
Test Item |
10 |
16 |
234 |
4.9 |
9.8 |
250 |
34 |
Blank Control |
0 |
16 |
234 |
-- |
-- |
250 |
35 |
Blank Control |
0 |
16 |
234 |
-- |
-- |
250 |
Results of the second experiment (main test):
Vessel No. |
Content |
Concentration in mg/L nominal |
O2consumption in mg/(L*min) |
O2consumption in mg/(L*h) |
Inhibition in % |
pH meeasured at the end of the test |
1 |
Blank Control |
0 |
0.7024 |
42.142 |
0 |
8.1 |
2 |
Blank Control |
0 |
0.7713 |
46.279 |
0 |
8.1 |
3 |
Positive Control |
5 |
0.4971 |
29.826 |
31.7 |
8.2 |
4 |
Positive Control |
10 |
0.3127 |
18.764 |
57.0 |
8.2 |
5 |
Positive Control |
20 |
0.1557 |
9.345 |
78.6 |
8.3 |
6 |
Positive Control |
40 |
0.0800 |
4.799 |
89.0 |
8.3 |
7 |
Blank Control |
0 |
0.6504 |
39.022 |
0 |
8.2 |
8 |
Blank Control |
0 |
0.7296 |
43.773 |
0 |
8.1 |
9 |
Test Item |
1000 |
0.0278 |
1.666 |
96.2 |
4.6 |
10 |
Test Item |
1000 |
0.0583 |
3.497 |
92.0 |
4.5 |
11 |
Test Item |
1000 |
0.0550 |
3.301 |
92.4 |
4.5 |
12 |
Test Item |
1000 |
0.0232 |
1.395 |
96.8 |
4.5 |
13 |
Test Item |
1000 |
0.0294 |
1.767 |
96.0 |
4.5 |
14 |
Test Item |
320 |
0.7550 |
45.299 |
-3.7 |
7.7 |
15 |
Test Item |
320 |
0.7025 |
42.148 |
3.5 |
7.8 |
16 |
Test Item |
320 |
0.7595 |
45.569 |
-4.3 |
7.8 |
17 |
Test Item |
320 |
0.7541 |
45.245 |
-3.6 |
7.8 |
18 |
Test Item |
320 |
0.7431 |
44.587 |
-2.1 |
7.8 |
19 |
Test Item |
100 |
0.7737 |
46.420 |
-6.3 |
8.0 |
20 |
Test Item |
100 |
0.7575 |
45.452 |
-4.1 |
8.0 |
21 |
Test Item |
100 |
0.6835 |
41.011 |
6.1 |
8.0 |
22 |
Test Item |
100 |
0.7034 |
42.204 |
3.4 |
8.1 |
23 |
Test Item |
100 |
0.6415 |
38.488 |
11.9 |
8.1 |
24 |
Test Item |
32 |
0.7666 |
45.997 |
-5.3 |
8.1 |
25 |
Test Item |
32 |
0.7391 |
44.346 |
-1.5 |
8.1 |
26 |
Test Item |
32 |
0.7621 |
45.727 |
-4.7 |
8.1 |
27 |
Test Item |
32 |
0.7468 |
44.810 |
-2.6 |
8.1 |
28 |
Test Item |
32 |
0.7860 |
47.163 |
-8.0 |
8.0 |
29 |
Test Item |
10 |
0.7508 |
45.048 |
-3.1 |
8.1 |
30 |
Test Item |
10 |
0.7357 |
44.141 |
-1.1 |
8.1 |
31 |
Test Item |
10 |
0.7544 |
45.265 |
-3.6 |
8.1 |
32 |
Test Item |
10 |
0.7528 |
45.168 |
-3.4 |
8.1 |
33 |
Test Item |
10 |
0.7735 |
46.411 |
-6.3 |
8.1 |
34 |
Blank Control |
0 |
0.7763 |
46.578 |
0 |
8.1 |
35 |
Blank Control |
0 |
0.7378 |
44.269 |
0 |
8.1 |
Content of Test Vessels in the second experiment (main test):
Vessel No. |
Content |
Concentration (mg/L) nominal |
Nutrient Solution (mL) |
Tap Water (mL) |
Stock Solution added (mL) |
Concentration of Stock Solution (mg/L) |
Inoculum (mL) |
1 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
2 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
3 |
Positive Control |
5 |
16 |
229 |
5 |
501 |
250 |
4 |
Positive Control |
10 |
16 |
224 |
10 |
501 |
250 |
5 |
Positive Control |
20 |
16 |
214 |
20 |
501 |
250 |
6 |
Positive Control |
40 |
16 |
194 |
40 |
501 |
250 |
7 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
8 |
Blank Control |
0 |
16 |
234 |
0 |
-- |
250 |
Vessel No. |
Content |
Concentration (mg/L) nominal |
Nutrient Solution (mL) |
Tap Water (mL) |
Amount added (mg) |
Calculated amount (mg) |
Inoculum (mL) |
9 |
Test Item |
1000 |
16 |
234 |
500.1 |
1000.2 |
250 |
10 |
Test Item |
1000 |
16 |
234 |
499.8 |
999.6 |
250 |
11 |
Test Item |
1000 |
16 |
234 |
500.3 |
1000.6 |
250 |
12 |
Test Item |
1000 |
16 |
234 |
500.6 |
1001.2 |
250 |
13 |
Test Item |
1000 |
16 |
234 |
499.8 |
999.6 |
250 |
14 |
Test Item |
320 |
16 |
234 |
159.6 |
319.2 |
250 |
15 |
Test Item |
320 |
16 |
234 |
160.4 |
320.8 |
250 |
16 |
Test Item |
320 |
16 |
234 |
160.9 |
321.8 |
250 |
17 |
Test Item |
320 |
16 |
234 |
159.8 |
319.6 |
250 |
18 |
Test Item |
320 |
16 |
234 |
159.6 |
319.2 |
250 |
19 |
Test Item |
100 |
16 |
234 |
50.2 |
100.4 |
250 |
20 |
Test Item |
100 |
16 |
234 |
50.1 |
100.2 |
250 |
21 |
Test Item |
100 |
16 |
234 |
50.2 |
100.4 |
250 |
22 |
Test Item |
100 |
16 |
234 |
50.3 |
100.6 |
250 |
23 |
Test Item |
100 |
16 |
234 |
49.7 |
99.4 |
250 |
Vessel No. |
Content |
Concentration (mg/L) nominal |
Nutrient Solution (mL) |
Tap Water (mL) |
Amount added (mg) |
Calculated amount (mg) |
Inoculum (mL) |
24 |
Test Item |
32 |
16 |
234 |
15.9 |
31.8 |
250 |
25 |
Test Item |
32 |
16 |
234 |
15.9 |
31.8 |
250 |
26 |
Test Item |
32 |
16 |
234 |
16.1 |
32.2 |
250 |
27 |
Test Item |
32 |
16 |
234 |
16.1 |
32.2 |
250 |
28 |
Test Item |
32 |
16 |
234 |
16.1 |
32.2 |
250 |
29 |
Test Item |
10 |
16 |
234 |
4.9 |
9.8 |
250 |
30 |
Test Item |
10 |
16 |
234 |
5.1 |
10.2 |
250 |
31 |
Test Item |
10 |
16 |
234 |
4.9 |
9.8 |
250 |
32 |
Test Item |
10 |
16 |
234 |
5.1 |
10.2 |
250 |
33 |
Test Item |
10 |
16 |
234 |
4.9 |
9.8 |
250 |
34 |
Blank Control |
0 |
16 |
234 |
-- |
-- |
250 |
35 |
Blank Control |
0 |
16 |
234 |
-- |
-- |
250 |
Results of the second experiment (main test):
Vessel No. |
Content |
Concentration in mg/L nominal |
O2consumption in mg/(L*min) |
O2consumption in mg/(L*h) |
Inhibition in % |
pH meeasured at the end of the test |
1 |
Blank Control |
0 |
0.7024 |
42.142 |
0 |
8.1 |
2 |
Blank Control |
0 |
0.7713 |
46.279 |
0 |
8.1 |
3 |
Positive Control |
5 |
0.4971 |
29.826 |
31.7 |
8.2 |
4 |
Positive Control |
10 |
0.3127 |
18.764 |
57.0 |
8.2 |
5 |
Positive Control |
20 |
0.1557 |
9.345 |
78.6 |
8.3 |
6 |
Positive Control |
40 |
0.0800 |
4.799 |
89.0 |
8.3 |
7 |
Blank Control |
0 |
0.6504 |
39.022 |
0 |
8.2 |
8 |
Blank Control |
0 |
0.7296 |
43.773 |
0 |
8.1 |
9 |
Test Item |
1000 |
0.0278 |
1.666 |
96.2 |
4.6 |
10 |
Test Item |
1000 |
0.0583 |
3.497 |
92.0 |
4.5 |
11 |
Test Item |
1000 |
0.0550 |
3.301 |
92.4 |
4.5 |
12 |
Test Item |
1000 |
0.0232 |
1.395 |
96.8 |
4.5 |
13 |
Test Item |
1000 |
0.0294 |
1.767 |
96.0 |
4.5 |
14 |
Test Item |
320 |
0.7550 |
45.299 |
-3.7 |
7.7 |
15 |
Test Item |
320 |
0.7025 |
42.148 |
3.5 |
7.8 |
16 |
Test Item |
320 |
0.7595 |
45.569 |
-4.3 |
7.8 |
17 |
Test Item |
320 |
0.7541 |
45.245 |
-3.6 |
7.8 |
18 |
Test Item |
320 |
0.7431 |
44.587 |
-2.1 |
7.8 |
19 |
Test Item |
100 |
0.7737 |
46.420 |
-6.3 |
8.0 |
20 |
Test Item |
100 |
0.7575 |
45.452 |
-4.1 |
8.0 |
21 |
Test Item |
100 |
0.6835 |
41.011 |
6.1 |
8.0 |
22 |
Test Item |
100 |
0.7034 |
42.204 |
3.4 |
8.1 |
23 |
Test Item |
100 |
0.6415 |
38.488 |
11.9 |
8.1 |
24 |
Test Item |
32 |
0.7666 |
45.997 |
-5.3 |
8.1 |
25 |
Test Item |
32 |
0.7391 |
44.346 |
-1.5 |
8.1 |
26 |
Test Item |
32 |
0.7621 |
45.727 |
-4.7 |
8.1 |
27 |
Test Item |
32 |
0.7468 |
44.810 |
-2.6 |
8.1 |
28 |
Test Item |
32 |
0.7860 |
47.163 |
-8.0 |
8.0 |
29 |
Test Item |
10 |
0.7508 |
45.048 |
-3.1 |
8.1 |
30 |
Test Item |
10 |
0.7357 |
44.141 |
-1.1 |
8.1 |
31 |
Test Item |
10 |
0.7544 |
45.265 |
-3.6 |
8.1 |
32 |
Test Item |
10 |
0.7528 |
45.168 |
-3.4 |
8.1 |
33 |
Test Item |
10 |
0.7735 |
46.411 |
-6.3 |
8.1 |
34 |
Blank Control |
0 |
0.7763 |
46.578 |
0 |
8.1 |
35 |
Blank Control |
0 |
0.7378 |
44.269 |
0 |
8.1 |
Description of key information
Based on several studies on edetic acid or their derivatives the lowest EC50 was 500 mg/L and the NOEC was 13 mg/L, respectively.
The EC50 value was confirmed by a study according to OECD 409 on CDTA containing the same ammonium groups. The NOEC of the latter is significantly higher. Nevertheless, in a worst case approach the lowest available NOEC is considered as relevant for the assessment.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 500 mg/L
- EC10 or NOEC for microorganisms:
- 13 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)