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EC number: 939-457-4 | CAS number: 1469983-50-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1st december 2008 - 26 may 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- One deviation was observed on the medium composition without affecting the results or the validity of the test. Lack of data on individual biodegradation of each replicate.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- Deviations:
- yes
- Remarks:
- one deviation was observed on the medium composition without affecting the results or the validity of the test
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: natural seawater
- Details on inoculum:
- The raw seawater was supplied by a submersible pump situated on Sutherland's pier on the west side of Flotta in Scapa Flow. The raw seawater is pumped continuously from a depth of two meter below low water spring tide level, before passing up to 1.8 kilometers of plastic pipes to a storage tank. Two smaller pumps move the water to three settlement tanks situated nine meters above floor level.
The seawater temperature varies between 6°C in the winter and 14°C in the summer.
The salinity is between 34 parts per thousand and 37 parts per thousand.
Five to seven days before test commencement, raw seawater passes by gravity through a 45 µm filter to the ageing tank stored in darkness.
Inoculum collected on 1 december 2008, seven days before test and at 10.6°C (température throughout ageing: 10.6 to 21.8°C) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 8.29 mg/L
- Initial conc.:
- 0.627 other: mg (O2)/mg
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD guideline (except for Na2HPO4, 2H2O at 29.92 g/Linstead of 33.30 g and for CaCl2 at 31.84 g/L instead of 27.50 g/L)
- Test temperature: 20 °C +/- 1°C
TEST SYSTEM
- Culturing apparatus: 270-276 ml glass BOD bottles containing 180-184 ml test medium
- Number of culture flasks:
Test material: 3 replicates per timepoint, minimum of two replicates per timepoint for data processing
Oxygen blank, reference, control, RBO: 3 replicates per timepoint, minimum of two replicates per timepoint for data processing
SAMPLING
- Sampling frequency: dissolved oxygen was measured at 7 day intervals during 28 days. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The test was considered as valid. All the validity criteria are fulfilled:
- Sodium benzoate attained more than 60% of degradation in 14 days (see table 4)
- The blank respiration did not exceed 30% of the oxygen in the test bottle after 28 days (table 5)
- BOD of the mixture of reference and test substances is less than the sum of the BOD of the separate solutions of the two substances (table 1) - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 57
- Sampling time:
- 28 d
- Details on results:
- The test item attained 57% degradation after 28 days.
According to the OECD 306 Guideline, if the result is positive (>70% DOC removal; >60% ThOD - theoretical oxygen demand), it may be concluded that there is a potential for biodegradation in the marine environment. Hence, the test item can't be considered to be biodegradable in seawater under the conditions of the test.
The results obtained from the toxicity control show an inhibition of 9% to seawater bacteria.. The toxicity control attained more than 60% of degradation after 14 days and the BOD of the mixture of reference and test substances is less than the sum of the BOD of the separate solutions of the two substances.
The reference substance, Sodium benzoate, attained 79% degradation after 14 days and 95% degradation after 28 days thereby confirming the suitability of the inoculum and test conditions. - Results with reference substance:
- The mean percentage degradation of the reference substance is 95% (greater than 60%) in 28 days of incubation. This result confirms the suitability of the inoculum and test conditions (see table 4).
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Based on O2 consumption, the biodegradation of the test item was 57% within 28 days incubation in seawater. Based on these results, the test item cannot be considered as biodegradable in seawater in the conditions of the test.
- Executive summary:
The ultimate aerobic biodegradability in seawater of the test item (= substance EC 939-455-3 in aqueous commercial product) was investigated in a GLP-compliant study performed in accordance with OECD Guideline 306 (Biodegradability in Seawater). In the test flasks containing the test item and seawater inoculum, a mean of 57% biodegradation was observed (based on O2 consumption) within the test period of 28 days. The test item was thus considered as not readily biodegradable in seawater under the test conditions.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- from 30 march 2010 to 30 may 2005
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: All the criteria fulfilled but the study was not performed on GLP principles
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14593
- Deviations:
- no
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge was collected from the aeration tank of a full-scale wasrewater treatment plant which treats predominantly domectic sewage (ETE Samambaia / Campinas-SP/Brazil).
The activated sludge was treated by allowing to settle three times for 30 minutes. For each setting the supernatant was rejected and the solids was redispersed in the mineral medium. In order to lower carbon organic content and to reduce the blanck CO2 evolution, the activated sludge was aerated overnight.
The final concentration of the teated activated sludge was adjusted to 1000 mg/L dry solids. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Mineral medium : As precribed in the 310 OECD guideline. The mineral medium was checked for pH, which was 7.51.
- Additional substrate : no
- Solubilising agent : no
- Test temperature : 22 +/- 2°C
- Continuous darkness : yes
- Agitation : the sealed test vessels were maintained in a rotary shaker, between 166-170 rpm (sufficient to keep the bottle contents well mixed and in suspension).
TEST SOLUTIONS
- Test substance :
A stock solution of the test solution containing 2000 mg C/l was prepared by adding 0.8670 g of the test substance into volumetric flask and completing the final volume to 100 ml with distilled water. One to one hunfred dilution of the test substance stock solution in the mineral medium was analyzed (apparatus : Shimadzu Model TOC 5000 carbon analyzer) and indicated a maean concentration of 21.12 mg C/L.
Sufficient test substance solution was prepared immediately before use by diluting the stock solution with the inoculum medium in order to give a final TOC concentration of 20 mg/L and 4 mg/L activated sludge dry solids. The measured pH was 7.55.
- Reference substance :
A stock solution of the reference substance containing 2000 mg C/l was prepared by adding 0.8569 g of the reference substance into a volumetric flask and completing the final volume to 250 ml with distilled water.
Sufficient reference substance solution was prepared immediately before use by diluting the stock solution with the inoculum medium in order to give a final TOC concentration of 20 mg/L .
INOCULATED MEDIUM
Sufficient inoculated medium was prepared immediately before by diluting the treated activated sludge (see details on inoculum) with mineral medium in order to achieve a concentration of 4 mg/L activated sludge dry solids.
TEST SYSTEM
- Culturing apparatus: Bottles of 120 ml capacity sealed with butyl rubber septa and aluminium crimp seals. The recommended headspace to liquid volume ratio of 1:2 (80 ml liquid volume to 120 ml-capacity bottles) was used.
- Number of culture flasks : 43 serum bottles were used for the study and were designated as follows :
- 13 blank controls : containing the inoculated medium (80 ml)
- 13 tests vessels : containing the test substance (at 21.12 mg/l of TOC) and the inoculated medium (80 ml)
- 13 reference vessels : containing reference substance (sodium benzoate) at 20 mg/l of TOC, the inoculum and the mineral medium (80 ml) (3)
- 4 inhibition controls : containing the test substance (at 21.12 mg /l of TOC) and reference substance (at 20 mg C/l of TOC) and the inoculated test medium
SAMPLING
SAMPLING FREQUENCY :
- Bottles were sacrified for Inorganic carbon (IC) analysis on the following times : days 3, 7, 14, 21 and 28
-Assay for CO2 production was conducted for duplicate bottles on days 3, 7, 14, 21 and five replicate bottles on day 28
SAMPLING METHOD :
The samples were prepared by injecting 1.0 ml of sodium hydroxide solution at 280 g/l through the septum of each bottle sampled, shaking on the incubation rotary shaker for 1h at the test temperature, removing the bottles from the shaker and allowing to settle. Suitable volume of the liquid phase were sampled and injected into a Shimatzu Model TOC 5000 inorganic carbon analyzer, which was calibrated using appropriate standards, and the concentrations of IC were recorded. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- purity : min. 99.5%; carbon content : 58.35%
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 65.28
- Sampling time:
- 28 d
- Details on results:
- Mean net CO2 values from the test substance vessels on days 3, 7, 14, 21 and 28 were 23.58, 46.95, 59.73, 63.87 and 65.28% respectively, of the theoretical amount, based on Inorganic Carbon analysis.
- Results with reference substance:
- The mean percentage degradation of the reference substance was 96.55% (greater than 60%) by the 14th day of incubation.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In the test flasks containing the test item and microbial inoculum, a mean of 65.3% of biodegradation was observed (based on the IC analysis) within the test period of 28 days. Therefore, the test item is considered as readily biodegradable (10 -day window does not apply to complex substances) under the test conditions.
- Executive summary:
The ultimate aerobic biodegradability in freshwater of the test item (= substance EC 939-457-4 in aqueous commercial product) was investigated in a non-GLP compliant study (De Nadai, 2005) performed in accordance with OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test) and ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test)). In the test flasks containing the test item and microbial inoculum, a mean of 65.3% biodegradation was observed (based on IC analysis) within the test period of 28 days. The test item was therefore considered as readily biodegradable under the test conditions (10-day window does not apply to complex substances).
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- from 25 february 2010 to 20 aprile 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: All the criteria fulfilled but the study was not performed on GLP principles
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14593
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- study performed in internal facilities without GLP-certification
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge was collected from the aeration tank of a full-scale wasrewater treatment plant which treats predominantly domectic sewage (ETE Samambaia / Campinas-SP/Brazil).
The activated sludge was treated by allowing to settle three times for 30 minutes. For each setting the supernatant was rejected and the solids was redispersed in the mineral medium. In order to lower carbon organic content and to reduce the blanck CO2 evolution, the activated sludge was aerated overnight.
The final concentration of the teated activated sludge was adjusted to 1000 mg/L dry solids. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Mineral medium : As precribed in the 310 OECD guideline. The mineral medium was checked for pH, which was 7.67.
- Additional substrate : no
- Solubilising agent : no
- Test temperature : 22 +/- 2°C
- Continuous darkness : yes
- Agitation : the sealed test vessels were maintained in a rotary shaker, between 166-170 rpm (sufficient to keep the bottle contents well mixed and in suspension).
TEST SOLUTIONS
- Test substance :
A stock solution of the test solution containing 2000 mg C/L was prepared by adding 1.7025 g of the test substance into volumetric flask and completing the final volume to 200 ml with distilled water. One to one hunfred dilution of the test substance stock solution in the mineral medium was analyzed (apparatus : Shimadzu Model TOC 5000 carbon analyzer) and indicated a maean concentration of 20.07 mg C/L.
Sufficient test substance solution was prepared immediately before use by diluting the stock solution with the mineral medium and with the treated activated sludge in order to give a final TOC concentration of 20 mg/L and 4 mg/L activated sludge dry solids. The measured pH was 7.64
- Reference substance :
A stock solution of the reference substance containing 2000 mg C/L was prepared by adding 0.6855 g of the reference substance into a volumetric flask and completing the final volume to 200 ml with distilled water.
Sufficient reference substance solution was prepared immediately before use by diluting the stock solution with the imineral medium and with the treated activated sludge in order to give a final TOC concentration of 20 mg/l .
INOCULUM MEDIUM
Sufficient inoculated medium was prepared immediately before use by diluting the treated activated sludge (see details on inoculum) with mineral medium in order to achieve a concentration of 4 mg/L activated sludge dry solids.
To reduce the carbon dioxide production of the blanks, the inoculated medium was preconditioned by aeration for 7 days at the test temperature. Water loss by evaporation was corrected during the aeration and especially before use
TEST SYSTEM
- Culturing apparatus: Bottles of 120 ml capacity sealed with butyl rubber septa and aluminium crimp seals. The recommended headspace to liquid volume ratio of 1:2 (80 ml liquid volume to 120 ml-capacity bottles) was used.
- Number of culture flasks : 43 serum bottles were used for the study and were designated as follows :
- 13 blank controls : containing the inoculated medium (80 ml)
- 13 tests vessels : containing the test substance (at 20.07 mg/l of TOC) and the inoculated medium (80 ml)
- 13 reference vessels : containing reference substance (sodium benzoate) at 20 mg/l of TOC, the inoculum and the mineral medium (80 ml)
- 4 inhibition controls : containing the test substance (at 20.07 mg /l of TOC) and reference substance (at 20 mg C/l of TOC) and the inoculated test medium
SAMPLING
SAMPLING FREQUENCY :
- Bottles were sacrified for Inorganic carbon (IC) analysis on the following times : days 4, 7, 14, 21 and 28
-Assay for CO2 production was conducted for duplicate bottles on days 4, 7, 14, 21 and five replicate bottles on day 28
SAMPLING METHOD :
The samples were prepared by injecting 1.0 ml of sodium hydroxide solution at 280 g/l through the septum of each bottle sampled, shaking on the incubation rotary shaker for 1h at the test temperature, removing the bottles from the shaker and allowing to settle. Suitable volume of the liquid phase were sampled and injected into a Shimatzu Model TOC 5000 inorganic carbon analyzer, which was calibrated using appropriate standards, and the concentrations of IC were recorded. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- purity : min. 99.5%; carbon content : 58.35%
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 62.59
- Sampling time:
- 28 d
- Details on results:
- Mean net CO2 values from the test substance vessels on days 4, 7, 14, 21 and 28 were 27.38, 40.22, 53.31, 60.84 and 62.59% respectively, of the theoretical amount, based on Inorganic Carbon analysis.
- Results with reference substance:
- The mean percentage degradation of the reference substance was 96.77% (greater than 60%) by the 14th day of incubation.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In the test flasks containing the test item and microbial inoculum, a mean of 62.6% of biodegradation was observed (based on the IC analysis) within the test period of 28 days. Therefore, the test item is considered as readily biodegradable (10 -day window does not apply to complex substances) under the test conditions.
- Executive summary:
The ultimate aerobic biodegradability in freshwater of the test item (= substance EC 939-457-4 in aqueous commercial product) was investigated in a non-GLP compliant study (De Nadai, 2010) performed in accordance with OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test) and ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test)). In the test flasks containing the test item and microbial inoculum, a mean of 62.6% biodegradation was observed (based on IC analysis) within the test period of 28 days. The test item was therefore considered as readily biodegradable under the test conditions (10-day window does not apply to complex substances).
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- from 25 february 2010 to 14 april 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- non-GLP study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test))
- Deviations:
- no
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge was collected from the aeration tank of a full-scale wasrewater treatment plant which treats predominantly domectic sewage (ETE Samambaia / Campinas-SP/Brazil).
The activated sludge was treated by allowing to settle three times for 30 minutes. For each setting the supernatant was rejected and the solids was redispersed in the mineral medium. In order to lower carbon organic content and to reduce the blanck CO2 evolution, the activated sludge was aerated overnight.
The final concentration of the teated activated sludge was adjusted to 1000 mg/L dry solids. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Mineral medium : As precribed in the 310 OECD guideline. The mineral medium was checked for pH, which was 7.67.
- Additional substrate : no
- Solubilising agent : no
- Test temperature : 22 +/- 2°C
- Continuous darkness : yes
- Agitation : the sealed test vessels were maintained in a rotary shaker, between 166-170 rpm (sufficient to keep the bottle contents well mixed and in suspension).
TEST SOLUTIONS
- Test substance :
A stock solution of the test solution containing 2000 mg C/l was prepared by adding 1.7012 g of the test substance into volumetric flask and completing the final volume to 200 ml with distilled water. One to one hunfred dilution of the test substance stock solution in the mineral medium was analyzed (apparatus : Shimadzu Model TOC 5000 carbon analyzer) and indicated a maean concentration of 19.56 mg C/L.
Sufficient test substance solution was prepared immediately before use by diluting the stock solution with the inoculum medium in order to give a final TOC concentration of 20 mg/L and 4 mg/L activated sludge dry solids. The measured pH was 7.68.
- Reference substance :
A stock solution of the reference substance containing 2000 mg C/l was prepared by adding 0.6855 g of the reference substance into a volumetric flask and completing the final volume to 200 ml with distilled water.
Sufficient reference substance solution was prepared immediately before use by diluting the stock solution with the inoculum medium in order to give a final TOC concentration of 20 mg/L .
INOCULATED MEDIUM
Sufficient inoculated medium was prepared immediately before by diluting the treated activated sludge (see details on inoculum) with mineral medium in order to achieve a concentration of 4 mg/L activated sludge dry solids.
TEST SYSTEM
- Culturing apparatus: Bottles of 120 ml capacity sealed with butyl rubber septa and aluminium crimp seals. The recommended headspace to liquid volume ratio of 1:2 (80 ml liquid volume to 120 ml-capacity bottles) was used.
- Number of culture flasks : 43 serum bottles were used for the study and were designated as follows :
- 13 blank controls : containing the inoculated medium (80 ml)
- 13 tests vessels : containing the test substance (at 19.56 mg/l of TOC) and the inoculated medium (80 ml)
- 13 reference vessels : containing reference substance (sodium benzoate) at 20 mg/l of TOC, the inoculum and the mineral medium (80 ml) (3)
- 4 inhibition controls : containing the test substance (at 19.56 mg /l of TOC) and reference substance (at 20 mg C/l of TOC) and the inoculated test medium
SAMPLING
SAMPLING FREQUENCY :
- Bottles were sacrified for Inorganic carbon (IC) analysis on the following times : days 4, 7, 14, 21 and 28
-Assay for CO2 production was conducted for duplicate bottles on days 4, 7, 14, 21 and five replicate bottles on day 28
SAMPLING METHOD :
The samples were prepared by injecting 1.0 ml of sodium hydroxide solution at 280 g/l through the septum of each bottle sampled, shaking on the incubation rotary shaker for 1h at the test temperature, removing the bottles from the shaker and allowing to settle. Suitable volume of the liquid phase were sampled and injected into a Shimatzu Model TOC 5000 inorganic carbon analyzer, which was calibrated using appropriate standards, and the concentrations of IC were recorded. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- purity : min. 99.5%; carbon content : 58.35%
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 63.2
- Sampling time:
- 28 d
- Details on results:
- Mean net CO2 values from the test substance vessels on days 4, 7, 14, 21 and 28 were 26.05, 40.36, 52.81, 60.94 and 63.16% respectively, of the theoretical amount, based on Inorganic Carbon analysis.
- Results with reference substance:
- The mean percentage degradation of the reference substance was 96.77% (greater than 60%) by the 14th day of incubation.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on the IC analysis results of the study, the biodegradation of the test item was 63.2 % within 28 days incubation. Based on these results, the test item is considered as readily biodegradable (10-day window does not apply to complex substances) in the conditions of the test.
- Executive summary:
The ultimate aerobic biodegradability in freshwater of the test item (= substance EC 939-455-3 in aqueous commercial product) was investigated in a non-GLP compliant study (De Nadai, 2010) performed in accordance with OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test) and ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test)). In the test flasks containing the test item and microbial inoculum, a mean of 63.2% biodegradation was observed (based on IC analysis) within the test period of 28 days. The test item was therefore considered as readily biodegradable under the test conditions (10-day window does not apply to complex substances).
.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- 1. HYPOTHESIS FOR THE ANALOGUE APPROACH
C8-C18 AAPHS and C12-18 AAPHS have the same functional groups, and general composition. The main variable resides in the alkyl chain distribution.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
Source chemical = C8-18 cocamidopropyl hydroxysultaine (EC 939-455-3).
Target chemical = C12-18 cocamidopropyl hydroxysultaine (EC 939-457-4).
3. ANALOGUE APPROACH JUSTIFICATION
The alkyl C-chain distribution of the source chemical significantly overlaps with the one of the target chemical, the C12-alkyl derivative being the major constituent in both chemicals. The structural differences in side C-chains is not expected to lead to significant differences on phys-chem properties.
4. DATA MATRIX
See "Documentation and scientific justification of the read-across approach" in section 13.2. - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 57
- Sampling time:
- 28 d
- Remarks on result:
- other: OECD 306 (seawater)
- Remarks:
- read-across from EC 939-455-3
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 63.2
- Sampling time:
- 28 d
- Remarks on result:
- other: OECD 310 (freshwater)
- Remarks:
- read-across from EC 939-455-3
Referenceopen allclose all
Table 1 - Average net oxygen consumption (BOD, mg O2.l-1)
|
Day |
||||
0 |
7 |
14 |
21 |
28 |
|
Oxygen consumption blank |
7.27 |
7.09 |
6.87 |
6.90 |
6.79 |
Sodium benzoate |
7.33 |
5.35 |
4.91 |
4.79 |
4.42 |
Test item |
7.31 |
4.69 |
4.35 |
4.11 |
3.80 |
Test item + Sodium benzoate |
7.24 |
3.12 |
2.60 |
2.39 |
1.92 |
Table 2 - Percentage degradation of the test item
Material |
100% BOD (mg/L) |
Measured BOD (mg/L) |
% degradation |
||||||
7 |
14 |
21 |
28 |
7 |
14 |
21 |
28 |
||
Test item |
5.2 |
2.40 |
2.52 |
2.79 |
2.98 |
46 |
49 |
54 |
57 |
Test item + Sodium benzoate |
7.7 |
3.98 |
4.27 |
4.51 |
4.87 |
52 |
56 |
59 |
63 |
Table 3 - Inhibition due to test item
Day |
Test item
BOD (mg/L) |
Sodium benzoate
BOD (mg/L) |
Sum of separate
BODs (mg/L) |
Test item + Sodium benzoate BOD (mg/L) |
Percentage inhibition % |
7 |
2.40 |
1.75 |
4.15 |
3.98 |
4 |
14 |
2.52 |
1.96 |
4.45 |
4.27 |
4 |
21 |
2.79 |
2.11 |
4.90 |
4.51 |
8 |
28 |
2.98 |
2.36 |
5.35 |
4.87 |
9 |
Table 4 - Reference substance degradation
Material |
100% BOD (mg/L) |
Measured BOD (mg/L) |
Percentage degradation % |
||||||
7 |
14 |
21 |
28 |
7 |
14 |
21 |
28 |
||
Sodium benzoate |
2.5 |
1.75 |
1.96 |
2.11 |
2.36 |
70 |
78 |
85 |
95 |
Table 5 - Blank Oxygen demand
Day |
Mean Dissolved Oxygen (mg/L) |
Mean BOD (mg/L) |
BOD (%) |
0 |
7.27 |
- |
- |
7 |
7.09 |
0.19 |
3 |
14 |
6.87 |
0.42 |
6 |
21 |
6.90 |
0.39 |
5 |
28 |
6.79 |
0.50 |
7 |
Table 1 - Cumulative (mg/L) Inorganic Carbon Evolved
Cumulative (mg/L) Inorganic Carbon Evolved |
|||||
Vessels contents |
3 days |
7 days |
14 days |
21 days |
28 days |
Test |
- |
- |
- |
- |
16.94 |
Test |
- |
- |
- |
- |
16.58 |
Test |
- |
- |
- |
- |
16.32 |
Test |
7.10 |
11.78 |
15.57 |
16.20 |
17.17 |
Test |
8.04 |
13.71 |
15.20 |
16.53 |
16.73 |
Mean |
7.57 |
12.75 |
15.39 |
16.37 |
16.75 |
Reference |
- |
- |
- |
- |
22.45 |
Reference |
- |
- |
- |
- |
22.73 |
Reference |
- |
- |
- |
- |
22.48 |
Reference |
19.02 |
21.82 |
22.17 |
22.27 |
22.08 |
Reference |
18.41 |
21.71 |
21.99 |
22.09 |
22.52 |
Mean |
18.72 |
21.77 |
22.08 |
22.18 |
22.45 |
Blank |
- |
- |
- |
- |
2.92 |
Blank |
- |
- |
- |
- |
2.89 |
Blank |
- |
- |
- |
- |
3.03 |
Blank |
2.77 |
3.09 |
2.75 |
2.83 |
2.91 |
Blank |
2.41 |
2.57 |
2.79 |
2.92 |
3.05 |
Mean |
2.59 |
2.83 |
2.77 |
2.88 |
2.96 |
Inhibition |
19.67 |
- |
- |
- |
39.19 |
Inhibition |
19.23 |
- |
- |
- |
39.05 |
Mean |
19.45 |
- |
- |
- |
39.12 |
Table 1 - Cumulative (mg/L) Inorganic Carbon Evolved
Cumulative (mg/L) Inorganic Carbon Evolved |
|||||
Vessels contents |
4 days |
7 days |
14 days |
21 days |
28 days |
Test |
- |
- |
- |
- |
15.79 |
Test |
- |
- |
- |
- |
15.33 |
Test |
- |
- |
- |
- |
15.89 |
Test |
7.40 |
10.33 |
13.34 |
15.39 |
15.91 |
Test |
7.13 |
10.88 |
13.72 |
15.23 |
15.55 |
Mean |
7.26 |
10.61 |
13.53 |
15.31 |
15.69 |
Reference |
- |
- |
- |
- |
22.76 |
Reference |
- |
- |
- |
- |
22.34 |
Reference |
- |
- |
- |
|
22.45 |
Reference |
17.41 |
21.47 |
22.26 |
22.59 |
23.18 |
Reference |
17.67 |
21.27 |
22.44 |
22.71 |
22.84 |
Mean |
17.54 |
21.37 |
22.35 |
22.65 |
22.71 |
Blank |
- |
- |
- |
- |
3.11 |
Blank |
- |
- |
- |
- |
2.97 |
Blank |
- |
- |
- |
- |
3.13 |
Blank |
1.63 |
2.68 |
2.92 |
3.04 |
3.22 |
Blank |
1.91 |
2.39 |
2.74 |
3.16 |
3.23 |
Mean |
1.77 |
2.53 |
2.83 |
3.10 |
3.13 |
Inhibition |
19.86 |
- |
- |
- |
35.16 |
Inhibition |
18.75 |
- |
- |
- |
36.21 |
Mean |
19.31 |
- |
- |
- |
35.69 |
Table 1 - Cumulative (mg/L) Inorganic Carbon Evolved
Cumulative (mg/L) Inorganic Carbon Evolved |
|||||
Vessels contents |
4 days |
7 days |
14 days |
21 days |
28 days |
Test |
- |
- |
- |
- |
|
Test |
- |
- |
- |
- |
|
Test |
- |
- |
- |
- |
|
Test |
6.67 |
10.52 |
13.37 |
15.15 |
15.97 |
Test |
7.06 |
10.34 |
12.95 |
14.89 |
15.36 |
Mean |
6.86 |
10.43 |
13.16 |
15.02 |
15.49 |
Reference |
- |
- |
- |
- |
22.76 |
Reference |
- |
- |
- |
- |
22.34 |
Reference |
- |
- |
- |
|
22.45 |
Reference |
17.41 |
21.47 |
22.26 |
22.59 |
23.18 |
Reference |
17.67 |
21.27 |
22.44 |
22.71 |
22.84 |
Mean |
17.54 |
21.37 |
22.35 |
22.65 |
22.71 |
Blank |
- |
- |
- |
- |
3.11 |
Blank |
- |
- |
- |
- |
2.97 |
Blank |
- |
- |
- |
- |
3.13 |
Blank |
1.63 |
2.68 |
2.92 |
3.04 |
3.22 |
Blank |
1.91 |
2.39 |
2.74 |
3.16 |
3.23 |
Mean |
|
|
|
|
3.13 |
Inhibition |
19.27 |
- |
- |
- |
35.37 |
Inhibition |
18.86 |
- |
- |
- |
35.11 |
Mean |
19.07 |
- |
- |
- |
35.24 |
Description of key information
The ultimate aerobic biodegradability in freshwater of the substance 1-Propanaminium, N-(3-aminopropyl)-2-hydroxy-N,N-dimethyl-3-sulfo-, N-(C12-18(even numbered) acyl) derivs., hydroxides, inner salts (EC 939-457-4) was investigated in two non-GLP compliant studies (De Nadai, 2005, 2010) performed in accordance with OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test) and ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test)). In the test flasks containing the substance and microbial inoculum, a mean biodegradation in the range 62.6-65.3% was observed (based on IC analysis) within the test period of 28 days. The substance was therefore considered as readily biodegradable under the test conditions (10-day window does not apply to complex substances).
Above results are consistent with freshwater and seawater biodegradability data obtained on similar substances:
- the ultimate aerobic biodegradability in freshwater of the analogue substance EC 939-455-3 was investigated in a non-GLP compliant study (De Nadai, 2010) performed in accordance with OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test) and ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test)). In the test flasks containing the analogue substance and microbial inoculum, a mean of 63.2% biodegradation was observed (based on IC analysis) within the test period of 28 days;
- the ultimate aerobic biodegradability in seawater of the analogue substance EC 939-455-3 was investigated in a GLP-compliant study performed in accordance with OECD Guideline 306 (Biodegradability in Seawater). In the test flasks containing the analogue substance and seawater inoculum, a mean of 57% biodegradation was observed (based on O2 consumption) within the test period of 28 days. Altough the analogue substance was considered as not readily biodegradable in seawater under the test conditions, the result of that test was consistent with the one from the ready biodegradability test (De Nadai, 2010) with a 28-day mineralization level very close to (but just below) the 60% mineralization threshold.
The alkyl C-chain distribution of the source substance EC 939-455-3 significantly overlaps with the one of the target substance EC 939-457-7, the C12-alkyl derivative being the major constituent in both chemicals. The structural differences in side C-chains is not expected to lead to significant differences on phys-chem properties.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The ultimate aerobic biodegradability in freshwater of the substance EC 939-457-4 (in aqueous commercial product) was investigated in two studies (De Nadaï, 2005, 2009) performed in accordance with standard methods, without deviations. Both studies are considered as reliable with restrictions (Klimisch 2) and were selected as key studies for the endpoint. The ultimate aerobic biodegradability in freshwater and seawater of a structural analogue (= substance EC 939-455-3 in aqueous commercial product) was investigated in two studies (De Nadaï, 2010, Finn, 2009) performed in accordance with standard methods, with no or minor deviations. Both studies are considered as reliable with restrictions (Klimisch 2) and were selected as supporting studies for the endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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