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EC number: 207-332-7 | CAS number: 463-04-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Test organisms (species):
- Daphnia magna
- Hardness:
- Between 140 and 250 mg.L-1
- Test temperature:
- 20 °C ± 2
- pH:
- Between 6 and 9
- Details on test conditions:
- Light/dark cycle: 16 h/8 h
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.317 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.317 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to an experimental study performed on isoamyl nitrite according to the OECD TG 202 and under GLP conditions, the 48h-EC50 was found to be 0.317 mg/L based on measured concentrations.
- Executive summary:
The objective of this study was to assess the effects of the test item Isoamyl Nitrite on the mobility of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004).
Since the substance is volatile and is not soluble in under conditions of the test, the test was carried out in closed bottles and the substance was added by injection through the septum.
Test item quantification was performed according to a validated analytical method (final report RRCo-000234_01) on a GC-MS/MS from Agilent (GC 7890b and MS triple quadrupole 7000b or c) as mass spectrometry detection was found suitable to measure Isoamyl Nitrite concentrations at 0 and 48 hours.
Results are the following:
Isoamyl Nitrite
EC50 (24h) 0.317 mg.L-1 (0.307 – 0.327 mg.L-1)
EC50 (48h) 0.317 mg.L-1 (0.307 – 0.327 mg.L-1)
The acute effects of Isoamyl Nitrite at the definitive nominal concentrations of 0; 0.31; 0.63; 1.25; 2.5 and 5 mg.L-1 to Daphnia magna were investigated under laboratory conditions.
Due to the instability of test item, the calculations were performed on the measured concentrations at initial time.
The study is valid since immobilization in the control was less than 10% (actual values: 0% in the definitive test), dissolved oxygen in the lowest concentration producing the maximum effect at the end of the test was ≥ 3 mg.L-1 (actual values: 8.5 mg.L-1 in the definitive test) and the EC50 with reference item over a period of 24 hours was between 0.6 and 2.1 mg.L-1 (actual value: 1.44 mg.L-1).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- SOURCE AND TARGET CHEMICAL(S)
The read-across has been performed between pentyl nitrite (CAS No. 463-04-7; target chemical) and isoamyl nitrite (CAS No. 110-46-3; source chemical). The RA is based on an experimental study performed on the source substance having a purity of 98.1%, in accordance with the OECD TG 202 and under GLP conditions.
ANALOGUE APPROACH JUSTIFICATION
The source substance and the target substance have the same chemical formula (C5H11NO2) and belong to the alkyl nitrites family. Like other alkyl nitrites, chemical and biological properties are mainly due to the nitrite group. Both target and source substances have similar physico-chemical properties.
For all these reasons, it is expected that the registered substance pentyl nitrite will have a similar ecotoxicity than the source substance isoamyl nitrite. The read-across approach between both alkyl nitrite substances is therefore considered relevant for this endpoint.
Isoamyl nitrite was found to be acutely toxic on aquatic invertebrates with an 48h-EC50 of 0.317 mg/L. The study was performed according to the OECD TG 202 under GLP conditions and all validity criteria were successful. This experimental study was therefore considered acceptable.
It is expected that similar results would be also found if a test was performed with pentyl nitrite. Due to the absence of experimental data on pentyl nitrite, it is therefore considered relevant to extrapolate results from the study on isoamyl nitrite to the registered substance. - Reason / purpose for cross-reference:
- read-across source
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.317 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.317 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on a read-across on an experimental study performed on another alkyl nitrite substance (isoamyl nitrite) in accordance with the OECD TG 202 and under GLP conditions, the registered substance pentyl nitrite is considered acutely toxic to aquatic invertebrates.
- Executive summary:
For that endpoint, a read-across was performed between pentyl nitrite (CAS No. 463-04-7; target chemical) and isoamyl nitrite (CAS No. 110-46-3; source chemical).
The source substance and the target substance have the same chemical formula (C5H11NO2) and belong to the alkyl nitrites family. Like other alkyl nitrites, chemical and biological properties are mainly due to the nitrite group. Both target and source substances have similar physico-chemical properties.
For all these reasons, it is expected that the registered substance pentyl nitrite will have a similar ecotoxicity than the source substance isoamyl nitrite. The read-across approach between both alkyl nitrite substances is therefore considered relevant for this endpoint.
An experimental study performed on the source substance isoamyl nitrite was available, and was conduted according to the OECD TG 202 under GLP conditions. The objective of this study was to assess the effects of the test item Isoamyl Nitrite on the mobility of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004).
Since the substance is volatile and is not soluble in under conditions of the test, the test was carried out in closed bottles and the substance was added by injection through the septum.
Test item quantification was performed according to a validated analytical method (final report RRCo-000234_01) on a GC-MS/MS from Agilent (GC 7890b and MS triple quadrupole 7000b or c) as mass spectrometry detection was found suitable to measure Isoamyl Nitrite concentrations at 0 and 48 hours.
Results are the following for the source substance Isoamyl Nitrite:
EC50 (24h) 0.317 mg.L-1 (0.307 – 0.327 mg.L-1)
EC50 (48h) 0.317 mg.L-1 (0.307 – 0.327 mg.L-1)
The acute effects of Isoamyl Nitrite at the definitive nominal concentrations of 0; 0.31; 0.63; 1.25; 2.5 and 5 mg.L-1 to Daphnia magna were investigated under laboratory conditions.
Due to the instability of test item, the calculations were performed on the measured concentrations at initial time.
The study is valid since immobilization in the control was less than 10% (actual values: 0% in the definitive test), dissolved oxygen in the lowest concentration producing the maximum effect at the end of the test was ≥ 3 mg.L-1 (actual values: 8.5 mg.L-1 in the definitive test) and the EC50 with reference item over a period of 24 hours was between 0.6 and 2.1 mg.L-1 (actual value: 1.44 mg.L-1).
It is expected that similar results would be also found if a test was performed with pentyl nitrite. Due to the absence of experimental data on pentyl nitrite, it is therefore considered relevant to extrapolate results from the study on isoamyl nitrite to the registered substance.
Referenceopen allclose all
Description of key information
Based on a read-across on an experimental study performed on another alkyl nitrite substance (isoamyl nitrite) in accordance with the OECD TG 202 and under GLP conditions, the registered substance pentyl nitrite is regarded as acutely toxic to aquatic invertebrates.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.317 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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