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Diss Factsheets
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EC number: 940-005-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to current test guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction product of Saccharose, Glycerine, biodiesel propoxylated
- EC Number:
- 940-005-3
- Molecular formula:
- Unspecified
- IUPAC Name:
- Reaction product of Saccharose, Glycerine, biodiesel propoxylated
- Details on test material:
- - Name of test material (as cited in study report): Reaction Product of Saccharose, Glycerine, biodiesel
propoxylated
- Physical state: Red to brown, clear, liquid
- Analytical purity: 100%
- Lot/batch No.: T41/012/13
- Expiration date of the lot/batch: 2014-06-15
- Storage condition of test material: Ambient (room temperature)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sample storage conditions before analysis: All samples were stored at room temperature until the start of the
analysis, if necessary. Prepared samples were stored at room
temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Five Water Soluble Fractions (WSF**) with nominal loadings of the test item in the range of 10.0
to 234 mg/L were prepared, set up in a geometric series with a factor of 2.2: 10.0 - 22.0 - 48.4 -
106 - 234 mg/L. For each loading level an appropriate amount of the test item was weighed out.
The test item was applied onto glass slides and inserted in brown glass bottles with appropriate
amounts of dilution water. The test item dispersions were shaken for 24 hours with 20 rpm at
room temperature. After a separation phase of 24 hours, undissolved particles were removed by
membrane filtration (pore size 0.45 μm). During filtration, the filter was always kept
covered. The resulting Water Soluble Fractions (WSF) were used
in the test. The method of preparation of the water soluble fractions
was based on a range finding test.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriel/a subcapitata HINDAK, SAG 61.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG)
Pflanzenphysiologisches lnstitut der Universitat Gottingen
Nikolausberger Weg 18, D-37073 Gottingen
- Age of inoculum (at test initiation): A three day old preculture, prepared in dilution water, was used
as inoculum. For the start of the test, the preculture was directly pipetted into each test media and the control.
- Method of cultivation: Fresh stocks are prepared every month on Z-Agar. Light intensity
amounted to 35-70 μE ·m-2 ·s-1 for 24 hours per day.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 0.24 mmol Ca+Mg/L
- Test temperature:
- 21 - 24 °C, controlled at± 2 °C
- pH:
- The pH-value were within the acceptable limits (8.14 - 8.38).
- Nominal and measured concentrations:
- Nominal: 10.0, 22.0, 48.4, 106, 234 mg/L
Measured: 9.13, 22.1, 50.2, 121, 263 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile Erlenmeyer flasks (vol. 250 ml) with cotton wool plugs
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 100 mL
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: Nominal: approximately 5 x 10³ - 10E04 cells/mL; Actual: 5.297 x 10³ cells/mL
- No. of vessels per concentration (replicates): 3 replicates per loading level
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to the guideline
- Intervals of water quality measurement: The pH-value at the start of the exposure was measured out of
one additional replicate per loading level and the control. At the
end of the exposure, it was measured from pooled replicates per
loading level and control. The room temperature was measured
continuously. Light intensity was measured prior to the start of the
exposure
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Light intensity and quality: Approximately 60 μE · m-2 · s-1, 24 hours/day light, Fluorescent tubes, OSRAM l 36 W/11-865, cool daylight
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: The cell density was measured daily via Chlorophyll-a-fluorescence,
excitation at 436 nm, emission at 685 nm. Dilution water was
used as background signal.
Microscopic evaluation of the cells at the start and end of the
exposure was carried out. The cells were checked for unusual cell
shapes, colour differences, differences in chloroplast morphology,
flocculation, adherence of algae to test containers and
agglutination of algae cells.
TEST CONCENTRATIONS
- Range finding study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 45.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- The toxicity of potassium dichromate (SIGMA, batch number MKBF6527V, purity 99.6 %, CAS RN
7778-50-9) to the unicellular freshwater green alga Pseudokirchneriel!a subcapitata was
determined over a period of 72 hours from 2013-10-22 to 2013-10-25.
Growth Rate inhibition ErC50: 0.904 [mg/L], (0-72 hours)
Yield inhibition EyC50: 0.491 [mg/L], (0-72 hours)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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