Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 234-439-6 | CAS number: 12003-78-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72h
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- certified by Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Str. 7, D-55116 Mainz, Germany
Test material
- Reference substance name:
- Aluminium, compound with nickel (1:1)
- EC Number:
- 234-439-6
- EC Name:
- Aluminium, compound with nickel (1:1)
- Cas Number:
- 12003-78-0
- Molecular formula:
- Al.Ni
- IUPAC Name:
- aluminum - nickel (1:1)
- Test material form:
- solid: particulate/powder
- Details on test material:
- dark grey fine powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For each treatment, 400 µL of the respective test item solution was mixed with the necessary amaount of algal pre-culture (1.85mL) to achieve a cell concentration of 4.6 x 10^3 cells/mL. In this mixture, the pH value was measured and samples for the analytical determination were taken.
The concentration of Al and Ni in the test vessels as contents of the test item were measured at the start and the end of the test.
Test solutions
- Vehicle:
- yes
- Remarks:
- demineralised water
- Details on test solutions:
- The nominal load of the highest concentration (32mg/L) was mixed with the corresponding amount of demineralised water and stirring vigorously for 96 +/- 3h. The resulting solution was filtrated through 0.45 µm nylon filters. The lower concentrations were prepared by dilution with demineralised water.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Unicellular green algae, Desmodesmus subspicatus Strain 86.81, obtained in January 2016 from MEM Sciencebridge GmbH (Institut für Pflanzenphysiologie, Universität Göttingen) and kept as stock culture.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 22.0 - 22.2°C
- pH:
- 7.6-7.9
- Nominal and measured concentrations:
- 1rst, 2nd, 3rd experiment: (nominal concentrations): 32/10/3.2/1/0.32 mg/L
4th, 5th experiment: (nominal concentrations): 10/3.2/1/0.32/0.1/0.032/0.01 mg/L
The determination of Al and Ni did not allow the calculation of the test item concentration, but showed clearly the presence of the test item in the solutions. - Details on test conditions:
- 5 experiments were performed.
The test vessels (glass flasks 65 ML total volume) were filled with 45 mL of the respective test solution and incubated open (covered with perforated plastic foil acting as a stopper) at light (4500 Lux) for 72h, shaken on an orbital shaker to keep algae in suspension. Before the start and every 24h, the cell number was determined by an electronic particle counter. After the end of the test (72h) the algae were examined microscopically. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, negative control: blank - nutrient medium
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- other: Yield
- Details on results:
- As a worst case scenario the results of the last (5th) experiment due to the strong inhibition of algal growth were taken for evaluation of the biological results. As inhibition of algal growth was only observable in the two highest treatments, only the three highest treatments were used for evaluation of the biological results.
- Results with reference substance (positive control):
- The 72h EC50s of potassium dichromate were determined in a seperate reference test. The values lay within the range of the laboratory: Growth rate: 0.73-1.10 mg/L, Yield: 0.21 - 0.66 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.