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EC number: 601-147-9 | CAS number: 111988-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 Aug - 05 Sep 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- BBA Part VI, 1-1
- Version / remarks:
- 1990
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: Natural soils collected on June 15 1995 passed through a 2 mm sieve and stored until used, as described in the BBA Guideline (1990) and ISO/DIS 1036-6 (1992).
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: treated with either 10 g ground quartz sand/kg dry wt soil (control) or a mixture of quartz sand and the test item (0.26 mg a.i. or 2.57 mg a.i./kg dw soil). The samples were mixed with pulverized lucerne-grass-green meal (5000 mg/kg dw soil) and quartz sand in 4 L aluminium containers by rolling on a gyrowheel mixer for 15 min at 50 rpm. - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 ± 2 °C
- Moisture:
- 40 % (Soil 1) and 43.6 % (Soil 2) water capacit
- Details on test conditions:
- TEST SYSTEM
- Testing facility: laboratory
- Test container (type, material, size): 500 mL brown glass bottles
- Amount of soil: 250 g dw
- No. of replicates per concentration: 1
- No. of replicates per control: 1
SOIL INCUBATION
- Method: series of individual subsamples
SOURCE AND PROPERTIES OF SUBSTRATE (soil 1)
- Organic carbon content (% dry weight): 0.6
- Nitrogen content (% dry weight): 0.06
- Geographical reference of sampling site: from Field plot F on the lab´s experimental farm Laacherhof
- Vegetation cover: Grass was planted in 1987, summer wheat in 1988, winter rye in 1988/89, winter barley in 1989/90, winter wheat in 1990/91, grass in Spring 1991, summer wheat in 1993 and winter rye in 1993/1994.
- Treatments with pesticides or fertilizers: Plant protection chemicals have not been used on this field since 1981.
- Accidental contamination: no
- Particle size distribution (in weight %): 2000-630 µm: 5.0, 630-200 µm: 53.2, 200-63 µm: 17.5, 63-20 µm: 9.3, 20-6.3 µm: 6.6, 6.3-2.0 µm: 4.7, 2.0-0 µm: 3.7
- Soil taxonomic classification: silty sand (according to DIN ‘schluffiger Sand’)
- pH: 5.8
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 34.5
- Maximum water holding capacity (in % dry weigth): 40
- Pretreatment of soil: mixed with pulverized lucerne-grass-green meal (5000 mg/kg soil dw)
- Microbial biomass: 187 mg microbial C/kg soil dw
SOURCE AND PROPERTIES OF SUBSTRATE (soil 2)
- Geographical reference of sampling site: from Field plot am Hohenseh 4 a on the lab's experimental farm Höfchen.
- Vegetation cover: 1985/86, summer barley; 1986/87, winter barley; 1987/88, oats; Fall 1988, winter barley ploughed up, perennial ryegrass planted.
- Treatments with pesticides or fertilizers: Plant protection chemicals have not been used on this field since 1988. The last fertilizer was applied in the Fall of 1988.
- Accidental contamination: no
- Particle size distribution (in weight %): 2000-630 µm: 1.8, 630-200 µm: 1.9, 200-63 µm: 3.0, 63-20 µm: 49.7, 20-6.3 µm: 26.7, 6.3-2.0 µm: 10.6, 2.0-0 µm: 6.3
- Soil taxonomic classification: silt (according to DIN ‘Schluff’)
- pH: 7.2
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 90.59
- Maximum water holding capacity (in % dry weigth): 43.6
- Microbial biomass: 187 mg microbial C/kg soil dw
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : determination of ammonium (indophenol green reaction), nitrite (samples were treated in acid medium with sulfanilic acid and N-1-naphthylethylenediaminedihydrochloride. The nitrite ions reacted to form a diazo dye, and the extinction of the dye was measured at 540 nm) and nitrates plus nitrite (nitrate ions were reduced to nitrite ions by hydrazine sulfate in the presence of Cu-ll-sulfate in alkaline medium. The nitrite ions were determined as described before) - Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 0.26 and 2.57 mg a.i./kg soil dw
- Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 2.57 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other:
- Remarks:
- Soil 1 and soil 2
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- The test item concentrations of 0.26 mg a.i./kg soil dw and the 10-fold overdose (2.57 mg a.i./kg soil dw) did not affect the nitrogen turnover in two different soils during 28-day experiment performed according to BBA Part VI, 1-1.
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- BBA Part VI, 1-1
- Version / remarks:
- 1990
- GLP compliance:
- yes (incl. QA statement)
- Vehicle:
- yes
- Remarks:
- quartz sand
- Details on preparation and application of test substrate:
- Sieved soil was treated with either 10 g ground quartz sand/kg dry wt soil (control) or a mixture of quartz sand and the test substance (0.26 mg or 2.57 mg test substance/kg dry wt soil). The samples were mixed in 4 liter aluminium containers by rolling on a gyrowheel mixer (Elte 650, J. Engelsmann AG, Ludwigshafen, Germany) for 15 min at 50 rpm.
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Nominal and measured concentrations:
- Nominal: 187.5 g and 1875 g test substance/ha corresponded to 0.26 mg and 2.57 mg test substance/kg dry wt soil
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.26 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Silty sand soil
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.75 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Silt soil
- Validity criteria fulfilled:
- not specified
- Conclusions:
- During 28-day experiments, 187.5 g a.i./ha (equivalent to 0.26 mg a.i./kg dry wt soil) and 1875 g a.i/ha (equivalent to 2.57 mg a.i. /kg dry wt soil) had no influence on soil respiration after addition of glucose to a silty sand (0.6 % org. C, pH (KCI) 5.7) or a silt (2.3 % org. C, pH (KCI) 7.1).
Referenceopen allclose all
In the time intervals analysed from 14-28 days exposure the difference in the daily nitrate-N rates does not exceed the trigger value of 25 %.
Table 1: Nitrogen mineralization in Soil 1, a silty sand, 0.6 %
org.C, pH (KCI) 5.8, after treatment with the test item and addition of
lucerne-grass-green meal (5000 mg/kg dry wt soil)
Days after treatment |
Control |
0.26 mg a.i./kg soil dw |
2.57 mg a.i./kg soil dw |
|||
Ammonium |
Nitrate |
Ammonium |
Nitrate |
Ammonium |
Nitrate |
|
[mg Nitrogen/kg soil dw]* |
||||||
0 |
2.85 ± 0.19 |
33.29 ±2.64 |
2.83 ± 0.30 |
32.57 ± 1.81 |
2.60 ± 0.24 |
32.32 ± 2.60 |
14 |
1.78 ± 0.47 |
14.98 ± 5.98 |
1.69 ± 0.08 |
16.62 ± 1.75 |
1.68 ± 0.14 |
18.92 ± 4.08 |
28 |
0.77 ± 0.05 |
32.59 ± 4.67 |
0.79 ± 0.03 |
30.36 ± 0.94 |
0.73 ± 0.05 |
32.63 ± 4.33 |
*Averages and standard deviations from 3 soil samples
Table 2: Nitrogen mineralization in Soil 2, a silt, 2.3 % org. C,
pH (KCI) 7.2, after treatment with the test item and addition of
lucerne-grass-green meal (5000 mg/kg drywt soil)
Days after treatment |
Control |
0.26 mg a.i./kg soil dw |
2.57 mg a.i./kg soil dw |
|||
Ammonium |
Nitrate |
Ammonium |
Nitrate |
Ammonium |
Nitrate |
|
[mg] Nitrogen/kg soil dw* |
||||||
0 |
4.18 ± 0.20 |
94.43 ± 0.60 |
4.17 ± 0.07 |
93.23 ± 1.06 |
4.14 ± 0.18 |
95.02 ± 0.81 |
14 |
1.64 ± 0.07 |
103.46 ± 1.53 |
1.67 ± 0.06 |
101.85 ± 0.72 |
1.68 ± 0.01 |
100.28 ± 2.04 |
28 |
1.15 ± 0.06 |
106.05 ± 4.08 |
1.15 ± 0.06 |
104.55 ± 1.51 |
1.13 ± 0.04 |
103.44 ± 1.01 |
*Averages and standard deviations from 3 soil samples
Table 3: Rates of nitrogen transformation/day for silty sand soil
Time interval (days) |
control |
0.26 mg/kg |
2.57 mg/kg |
||
mg nitrogen / kg dry weight soil / time interval / day |
|||||
nitrate (MV) |
nitrate (MV) |
% Diff. |
nitrate (MV) |
% Diff. |
|
0-14 |
-1,31 |
-1,14 |
-12,89 |
-0,96 |
-26,82 |
14-28 |
1,26 |
0,98 |
-21,98 |
0,98 |
-22,15 |
Table 4: Rates of nitrogen transformation/day for silt soil
Time interval (days) |
control |
0.26 mg/kg |
2.57 mg/kg |
||
mg nitrogen / kg dry weight soil / time interval / day |
|||||
nitrate (MV) |
nitrate (MV) |
% Diff. |
nitrate (MV) |
% Diff. |
|
0-14 |
0,64 |
0,62 |
-4,54 |
0,38 |
-41,75 |
14-28 |
0,19 |
0,19 |
4,25 |
0,23 |
22,01 |
Table 5: Validity criteria for OECD 216 (2000)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The variation of nitrate concentrations between replicate control samples should be less than ± 15%. |
CV% < 25% after 28 days |
yes |
Description of key information
NOEC (28 d): ≥ 2.57 mg/kg soil dw (nominal, soil microflora, BBA Part VI, 1-1)
Key value for chemical safety assessment
Additional information
Two studies are available assessing the effects of 3-(2-chlor-5-pyridyl-methyl)-cyanimino-1,3-thiazolidin on soil microorganisms according to BBA Part VI, 1-1.
In the first study, 2 lucerne-grass-green-meal amended soils (5000 mg/kg) were treated with 187.5 g test item/ha (corresponding to 0.26 mg a.i./kg soil dw) and the 10-fold overdose of the test item (1875 g a.i./ha, corresponding to 2.57 mg a.i./kg soil dw). Immediately after treatment and after 14 and 28 days, the soils were analyzed for their content of ammonium-N, nitrite-N and nitrate-N plus nitrite-N. The analyzes revealed that the test item treatment had no influence on the turnover of nitrogen in both soils (NOEC (28 d) ≥ 2.57 mg a.i./kg soil dw).
The second study supports the results of the key study. The test substance (0.26 mg or 2.57 mg test substance/kg dry wt soil) showed no influence on soil respiration after addition of glucose to two soils during 28 days of incubation (NOEC (28 d) ≥ 2.57 mg a.i./kg soil dw).
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