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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2020-05-01 to 2020-05-07, with the definitive exposure from 2020-05-04 to 2020-05-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Determination of
Samples were analyzed with an LC-MS/MS method, implemented
the test item under non-GLP and documented finally in the GLP raw data. The method was validated under GLP based on the criteria set forth by SANCO/3029/99 rev.4 11/07/00.
Sampling schedule
All concentration levels and the control were analytically verified via LC-MS/MS at the start (0 hours) and at the end of the exposure (72 hours) with algae.
Sampling and pre-treatment
At the start, samples were taken from one additional replicate of the test item concentration and the control and at the end samples were taken from pooled replicates. - Vehicle:
- no
- Details on test solutions:
- Preparation of the saturated solution
A saturated solution with a nominal loading rate of 100 mg test item/L was prepared once 72 ± 1 hour prior to the start of the exposure. An appropriate amount of the test item was pipetted onto the surface of the dilution water. For this purpose, the density was taken into account (0.98 g/cm3). A slow stirring procedure was applied for 72 ± 1 hour at room temperature in the dark. After a separation phase of 1 hour (at room temperature), the saturated solution was removed by siphoning (from the approximate bottom of the glass flask).
Preparation of the test item solutions
Out of the saturated solution 5 concentrations were tested in a geometric series with a dilution factor of 2:
6.25 – 12.5 – 25 – 50 – 100% of the saturated solution. The dilution levels are based on the results of a preliminary range finding test (non-GLP).
Control
Six replicates (without test item) were tested under the same test conditions as the test item replicates. - Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- TEST ORGANISM
- Common name: Skeletonema costatum (GREVILLE) Cleve 1873, CCAP 1077/1C
- Strain: CCAP 1077/1C
- Source (laboratory, culture collection):
Culture Collection of Algae and Protozoa (CCAP)
SAMS Research Services Ltd
Dunstaffnage Marine Laboratory
Dunbeg, OBAN; Argyll PA37 1QA; Scotland, UK
- Age of inoculum (at test initiation): 4 days
- Method of cultivation: Fresh stocks were prepared every month in dilution water. Light intensity amounted to 2567 – 5130 lux corresponding to 35 - 70 µE ∙ m-2 ∙ s-1 for 24 h per day. - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- Not specified
- Test temperature:
- min.: 18.5 max.: 19.5 mean value: 19.0
- pH:
- Geometric mean measured test item concentration Nominal test item loading pH-value
[µg/L] [mg/L] Start; 0 hours End; 72 hours
165 100 8.21 7.99
53.2 50.0 8.27 8.06
22.7 25.0 8.32 8.14
10.4 12.5 8.34 8.14
4.52 6.25 8.35 8.14
Control 8.37 8.14 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured
- Nominal and measured concentrations:
- Nominal test item concentrations 6.25 - 12.5 - 25.0 - 50.0 - 100 % of the saturated solution, corresponding to nominal loadings of 6.25 to 100 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 40 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 1 x 10^4 cells/mL, Actual: mean 9439 cells/mL
- Control end cells density: Mean 161374 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
According to the CCAP Administration double concentrated medium (F/2 + Si) with natural seawater (salinity of 30 +/- 5 S):
Composition of Dilution Water
Component Concentration [mg/L]
NaNO3 150
NaH2PO4 H2O 10
Na2SiO3 9 H2O 60
Titriplex III 8.32
FeCl3 6 H2O 6.30
CuSO4 5 H2O 0.02
ZnSO4 7 H2O 0.044
CoCl2 6 H2O 0.02
MnCl2 4 H2O 0.36
Na2MoO4 4 H2O 0.012
Vitamin B1 (Thiamin hydrochloride) 0.2
Vitamin H (Biotin) 0.1 µg/L
Vitamin B12 (Cyanocobalamine) 0.1 µg/L
pH 8.0 +/- 0.2
The pH of the test medium has to be 8.0 +/- 0.2 and was adjusted prior to testing with addition of 1 N NaOH and HCl, if necessary.
Characterization Data of Natural Seawater
Location Alfred-Wegener-Institut,
Biologische Anstalt Helgoland,
Kurpromenade 201, D-27498 Helgoland Latitude:54.11°, Longitude: 7.53°
Arrival Date 2019-09-24
pH 7.83
Salinity [S] 32.7
Pretreatment
The natural seawater was filtrated (membrane filter, 0.2 µm RC), adjusted to a salinity of 30 S with demineralized water and sterilized (autoclave d at 121 °C) until addition of the F/2 + Si components
Storage conditions Room temperature
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: Approximately 2567 – 3300 lux, measured: 2578 - 3046 lux with a mean of 2775 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
-Chlorophyll a-fluorescence
The cell density was measured daily via Chlorophyll a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal. No self-fluorescence was observed in the preliminary range finding test at the saturated solution. - Reference substance (positive control):
- yes
- Remarks:
- (Potassium dichromate)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22.7 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22.7 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 53.2 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 53.2 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 38.9 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CI: 25.3 - 48.4
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 45.5 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CI: 30.2 - 51.1
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 63.4 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CI: 55.9 - 99.5
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 32.5 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield inhibition
- Remarks on result:
- other: CI: 26.2 - 39.2
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 36.1 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield inhibition
- Remarks on result:
- other: CI: 29.4 - 41.8
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 46.5 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield inhibition
- Remarks on result:
- other: CI: 40.0 - 50.5
- Duration:
- 72 h
- Dose descriptor:
- other: NOEL
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: LOEL
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: NOEL
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- other: LOEL
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- other: EL10
- Effect conc.:
- 38.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Cl: 27.3 - 46.2
- Duration:
- 72 h
- Dose descriptor:
- other: EL20
- Effect conc.:
- 44.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Cl: 31.7 - 48.4
- Duration:
- 72 h
- Dose descriptor:
- other: EL50
- Effect conc.:
- 57.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Cl: 52.1 - 77.7
- Duration:
- 72 h
- Dose descriptor:
- other: EL10
- Effect conc.:
- 33.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: Cl: 28.1 - 38.9
- Duration:
- 72 h
- Dose descriptor:
- other: EL20
- Effect conc.:
- 36.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: Cl: 30.9 - 41.0
- Duration:
- 72 h
- Dose descriptor:
- other: EL50
- Effect conc.:
- 44.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: Cl: 39.8 - 47.9
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: The test media were clear throughout exposure period (possible turbidity related to algae growth not taken into account).
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- The toxicity of 3,5-Dichlorophenol (SIGMA-ALDRICH, batch number MKBZ0947V, purity 100.0%, CAS RN 591-35-5) to the marine diatom alga Skeletonema costatum was determined over a period of 72 hours from 2020-03-09 to 2020-03-12. The reference item toxicity is in the valid range which was established by calculation of the average of the historic reference data since 2011, and the limits were set using the threefold standard deviation of these values.
EC50-Values of the Reference Item
based on nominal concentrations [mg/L], (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Growth Rate inhibition
ErC50 1.62 1.70 ± 0.910
95% confidence interval 1.58 – 1.65
Yield inhibition
EyC50 1.17 1.40 ± 0.914
95% confidence interval 1.07 – 1.27
SD = Standard deviation - Reported statistics and error estimates:
- EC/EL-values and statistical analyses
EC/EL10, EC/EL20 and EC/EL50-values with confidence intervals of growth rate and yield inhibition after 72 hours were calculated by sigmoidal dose-response regression with the software GraphPad Prism.
NOECNOEL, LOEC/LOEL and statistical analyses
The NOEC/NOEL and LOEC/LOEL were determined by calculation of statistically significant differences of growth rate and yield using Dunnett’s Method. A Shapiro-Wilk’s Normality test and a Brown-Forsythe’s Equal Variance test were done first. P-values for both Normality and Equal Variance tests are 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is = 0.01. Data were transformed for growth rate (Y = Y squareroot).
Software
The data for the tables in this report were computer-generated and have been rounded for presentation from the full derived data. Consequently, if calculated manually based on the given data minor deviations may occur from these figures.
Calculations were carried out using software
•Excel, MICROSOFT CORPORATION
•SigmaPlot, SPSS INC.
•GraphPad Prism, GRAPHPAD SOFTWARE, INC. - Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol was found to inhibit the growth of the marine diatom alga Skeletonema costatum after 72 hours with the following effect values (based on geometric mean measured concentrations and nominal test item loadings): The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 63.4 (55.9 – 99.5) and 46.5 (40.0 – 50.5) µg test item/L, respectively. The EL50-values with 95% confidence intervals for inhibition of growth rate (ErL50) and yield (EyL50) after 72 hours were 57.5 (52.1 – 77.7) and 44.8 (39.8 – 47.9) mg test item/L, respectively. The NOEC/NOEL-values for both inhibition of growth rate and yield after 72 hours were 22.7 µg test item /L, corresponding to 25.0 mg test item/L.
- Executive summary:
The toxicity of Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol (Batch no.: ESD0033040) to the marine diatom alga Skeletonema costatum was determined according to the principles of DIN EN ISO-Guideline 10253 from 2020-05-01 to 2020-05-07, with the definitive exposure from 2020-05-04 to 2020-05-07 at the test facility. The aim of the study was the determination of NOEC/NOEL, LOEC/LOEL, EC/EL10, EC/EL20 and EC/EL50-values of growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 9439 cells/mL. A saturated solution of 100 mg test item/L was prepared once 72 ± 1 hour prior to the start of the exposure. An appropriate amount of the test item was pipetted onto the surface of the dilution water. For this purpose, the density was taken into account (0.98 g/cm3). A slow stirring procedure was applied for 72 ± 1 hour at room temperature in the dark. After a separation phase of 1 hour (at room temperature), the saturated solution was removed by siphoning (from the approximate bottom of the glass flask). Five concentrations were tested in a geometrical series with a dilution factor of 2 (nominal): 6.25 - 12.5 - 25.0 - 50.0 - 100 % of the saturated solution, corresponding to nominal loadings of 6.25 to 100 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. The environmental conditions were within the acceptable limits.
The concentrations of the test item, Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol, were analytically verified by LC-MS/MS at the start and the end of the exposure of all test concentrations and the control. The analytical method was validated according to SANCO/3029/99 rev.4. The measured concentrations of the test item, Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol, at the start of the exposure (0 hours) were in the range of 24.6 to 713 µg/L. The measured concentrations of the test item at the end of the exposure (72 hours) were in the range of 0.828 to 38.2 µg/L, corresponding to 3 to 5% of the initially measured values.
NOEC, LOEC and ECx-values and 95% Confidence Intervals of Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol (0 - 72 hours)
based on the geometric mean measured test item concentration [mg/L]
Growth Rate Inhibition
Geometric mean measured test item concentration [µg/L] Growth Rate Inhibition
Nominal test item loading [mg/L]
NOEC/NOEL 22.7 25.0
LOEC/LOEL 53.2 50.0
ErC10 / ErL10 38.9 (25.3 – 48.4) 38.8 (27.3 – 46.2)
ErC20 / ErL20 45.5 (30.2 – 51.1) 44.1 (31.7 – 48.4)
ErC50 / ErL50 63.4 (55.9 – 99.5) 57.5 (52.1 – 77.7)
Yield Inhibition
Geometric mean measured test item concentration [µg/L] Yield Inhibition
Nominal test item loading [mg/L]
NOEC/NOEL 22.7 25.0
LOEC/LOEL 53.2 50.0
EyC10 / EyL10 32.5 (26.2 – 39.2) 33.5 (28.1 – 38.9)
EyC20 / EyL20 36.1 (29.4 – 41.8) 36.5 (30.9 – 41.0)
EyC50 / EyL50 46.5 (40.0 – 50.5) 44.8 (39.8 – 47.9)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 08.03.07 - 16.03.07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Version / remarks:
- 2006
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Test item: Dodicor V 5654
Batch: 07-YS-019
Date test item received: 12.02.07 - Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- The strain of Skeletonema costatum (Greville) used in these tests is NIVA BAC 1. The culture mediUill
is prepared from natural seawater obtained from a depth of ca. 130 m. The salinity is adjusted to 30 ±5
%0. The culture medium is prepared from natural seawater by addition of 15 ml ISO nutrient stock
solution 1, 0.5 ml nutrient stock solution 2, and 1 ml nutrient stock solution 3 to 1 litres of seawater
(the compositions of the ISO nutrient media are given in Table 1 on page 12 of this report). The preculture
is maintained under the same conditions as used in the test (see Summary of Test Conditions).
Culture is semi-continuous under continuous even fluorescent lighting at an intensity of 60 - 90 )lmol
m-2
S-l, with continuous agitation on an orbital shaker.
Temperature is maintained at 20±2.0°C. Algal cultures are used for tests when they are detennined to
be in a phase of exponential growth. Cell density in the pre-culture is measured immediately before a
test in order to calculate the required inoculum volume. - Test temperature:
- 20 +- 2 °C
- pH:
- start 6.13 - 8.09
end 7.40 - 8.37 - Salinity:
- Natural seawater salinity 34 promille
- Nominal and measured concentrations:
- 55.9 - 100 -321 - 559 - 1000 mg/L and a control
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 412 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 321 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- A growth inhibition test was performed with Skeletonema costatum using Dodicor V 5654 at
nominal concentrations of 55.9- 1000 mg r l
. The following results were obtained:
72-h EC50: 412 mg/L
24-h EC50 : 382 mg/L
48-h EC50 : 400 mg/L
72-hNOEC: 321 mg/L - Executive summary:
A growth inhibition test was performed withSkeletonema costatumusing Dodicor V 5654 at
nominal concentrations of 55.9- 1000 mg rl
.The following results were obtained:
72-hEC50: 412 mg/L
24-hEC50 : 382 mg/L
48-hEC50 : 400 mg/L
72-hNOEC: 321 mg/L
Comments: Stock solution of 1054 mg!l of the test item was prepared according to procedure D, as
described in the OSPAR Guidelines for Toxicity Testing of Substances and Preparations
Used and Discharged Offshore. See pages10-11of this report. Test solutions were
prepared from the Water Accommodated Fraction (WAF).
Note:test results obtained when the test item is prepared according to procedure A refer to
the nominal concentrations of the test item as stated. Test results obtained when the test
item is prepared according to procedures B, C or D refer only to Water-Accommodated
Fractions of the test item prepared at the nominal concentrations of the test item stated.
ECso (48 h) value has been calculated manually from the dose-response curve (see
comments to Table 3 on Page 17 of this report).
Optimal pH range for the algae test is 7.8 - 8.2. The two highest test concentrations of
Dodicor V 5654 showed pH-values below this range at the start of the test.
72-h NOEC refers to the highest test concentration showing no statistically significant
Referenceopen allclose all
Microscopic evaluation of the cells at the start and the end of exposure revealed no morphological abnormalities. All effect values given are based on the geometric mean measured test item concentrations and the nominal loadings of Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol, respectively.
Cell Densities
Geometric mean measured test item concentration Nominal test item loading Replicate Cell density [cells/mL]
[µg/L] [mg/L] No. 0 hours 24 hours 48 hours 72 hours
165 100 1 9439 < LOQcell density < LOQcell density 5065
2 9439 < LOQcell density 6871 18329
3 9439 2947 5282 19764
Mean 9439 n.a. n.a. 14386
53.2 50.0 1 9439 4501 23699 58610
2 9439 5612 20903 63855
3 9439 5134 32766 64994
Mean 9439 5082 25789 62486
22.7 25.0 1 9439 17851 65637 159684
2 9439 18836 62961 183615
3 9439 20425 65056 174583
Mean 9439 19037 64551 172627
10.4 12.5 1 9439 27481 71663 185847
2 9439 22532 72460 178478
3 9439 19525 84932 194652
Mean 9439 23179 76352 186326
4.52 6.25 1 9439 24525 68826 172419
2 9439 22071 67767 162111
3 9439 22515 71446 193695
Mean 9439 23037 69346 176075
Control 1 9439 26461 72745 152771
2 9439 21962 80034 151142
3 9439 24713 70398 185893
4 9439 24161 65899 153648
5 9439 22122 69054 155060
6 9439 24183 73189 169730
Mean 9439 23934 71887 161374
n.a. = not applicable
LOQcell density = 2712
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to control values are marked (+), not significant differences are marked (-).
Geometric mean measured test item concentration Nominal test item loading Repl. Growth rate Inhibition of growth rate Yield Inhibition of yield
[µg/L] [mg/L] No. [d-1] [%] [cells/mL] [%]
165 100 1 -0.21 100 -4374 100
2 0.22 77 8890 94
3 0.25 74 10325 93
Mean (+) 0.09 84 (+) 4947 96
53.2 50.0 1 0.61 36 49171 68
2 0.64 33 54416 64
3 0.64 32 55555 63
Mean (+) 0.63 33 (+) 53047 65
22.7 25.0 1 0.94 0 150245 1
2 0.99 -5 174176 -15
3 0.97 -3 165144 -9
Mean (-) 0.97 -2 (-) 163188 -7
10.4 12.5 1 0.99 -5 176408 -16
2 0.98 -4 169039 -11
3 1.01 -7 185213 -22
Mean (-) 0.99 -5 (+)* 176887 -16
4.52 6.25 1 0.97 -2 162980 -7
2 0.95 0 152672 0
3 1.01 -7 184256 -21
Mean (-) 0.97 -3 (-) 166636 -10
Control 1 0.93 143332
2 0.92 141703
3 0.99 176454
4 0.93 144209
5 0.93 145621
6 0.96 160291
Mean 0.95 151935
* = statistical significance is caused by growth stimulation
Section-by-Section and Average Specific Growth Rates of the Control Group
(0 - 72 hours)
Replicate No. Specific growth rate [d-1] Mean
(0 - 72 hours) SD
± CV
[%] Mean CV [%]
section-by-section
0 - 24 hours 24 - 48 hours 48 - 72 hours
Control 1 1.03 1.01 0.74 0.93 0.161 17.4 16.7
2 0.84 1.29 0.64 0.92 0.336 36.3
3 0.96 1.05 0.97 0.99 0.046 4.67
4 0.94 1.00 0.85 0.93 0.079 8.48
5 0.85 1.14 0.81 0.93 0.179 19.2
6 0.94 1.11 0.84 0.96 0.135 14.0
Mean 0.945
SD ± 0.03
CV [%] 2.90
SD = Standard deviation CV = Coefficient of variation
Description of key information
Two studies according ISO 10253 Guidline with Skeletonema costatum are available.
In one study, Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol was found to inhibit the growth of the marine diatom alga Skeletonema costatum after 72 hours with the following effect values (based on geometric mean measured concentrations and nominal test item loadings): The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 63.4 (55.9 – 99.5) and 46.5 (40.0 – 50.5) µg test item/L, respectively. The EL50-values with 95% confidence intervals for inhibition of growth rate (ErL50) and yield (EyL50) after 72 hours were 57.5 (52.1 – 77.7) and 44.8 (39.8 – 47.9) mg test item/L, respectively. The NOEC/NOEL-values for both inhibition of growth rate and yield after 72 hours were 22.7 µg test item /L, corresponding to 25.0 mg test item/L.
A second growth inhibition test was performed with Skeletonema costatum using Dodicor V 5654 at
nominal concentrations of 55.9- 1000 mg r l
. The following results were obtained:
72-h EC50: 412 mg/L
24-h EC50 : 382 mg/L
48-h EC50 : 400 mg/L
72-hNOEC: 321 mg/L
Key value for chemical safety assessment
- EC50 for marine water algae:
- 63.4 µg/L
- EC10 or NOEC for marine water algae:
- 38.9 µg/L
Additional information
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