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EC number: 203-733-6 | CAS number: 110-05-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2008-06-02 to 2008-07-11
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed following OECD Guideline 202, under GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2008-11-12
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: 26 hPa (at 20°C) - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, and 150 mg/L
- Sampling method: The test item (52.59 mg) was dissolved in acetonitrile and made up to the mark in a 50 mL volumetric flask to prepare a stock solution of 1052 mg/L. Defined volumes of this stock solution were diluted with acetonitrile/water (v/v; 1/1) to obtain calibration solutions of the test item in the range of 2.63 to 168 mg/L. A second series of calibration solutions in the range of 1.09 to 87.0 mg test item /L, based on a stock solution of 1087 mg/L was prepared. The total calibration range used was 1.09 to 168 mg/L. These solutions were used to calibrate the HPLC-system.
- Sample storage conditions before analysis: no storage period. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:For determination of the actual concentrations of the test item Di-t-butyl peroxide, quadriplicate samples were taken from each treatment before the test start and at the end of the test after 48 hours. For the 48-hour stability samples, the contents of the respective replicates were combined prior to sampling. Immediately after sampling, acetonitrile in the ratio of 1:1 was added to all samples of the single test concentration from both sampling times to stabilize these samples. Thereafter, duplicate samples of the single test concentration were immediately analyzed. From the control, only one of the duplicate samples from both sampling times was analyzed immediately after sampling. The other duplicate samples of each treatment were stored deep-frozen (at about -20 °C). These samples were not analyzed.
- Eluate: distilled
- Differential loading: cf. Method description above
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnid
- Strain: Clone 5
- Source: University of Sheffield/UK (in 1992)
- Age at study initiation (mean and range, SD): 6-24 hours old.
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data
- Method of breeding: The clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests.
- Feeding during test: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no data
- Hardness:
- no data
- Test temperature:
- 20°C
- pH:
- 7.6-7.8
- Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Nominal and measured concentrations:
- nominal conc.: 0, and 152 mg/L
measured conc.: 0, and 73.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass tubes
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 50 mL of test medium
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter): no data
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 5
- Biomass loading rate: no data
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no data
- Photoperiod: 16:8
- Light intensity and quality: from 500 to 630 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter: Coulter Conter, Model ZM
- Chlorophyll measurement: none
- Other: none
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 150
- Test concentrations: 0, and 150 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 73.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 73.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 73.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 73.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- no data
- Results with reference substance (positive control):
- For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in March 2008 (48-hour EC50: 0.73 mg/L, study B83744) indicated that the sensitivity of the test organisms was within the internal historical range (48-hour EC50 from 1996 to 2008: 0.53 1.1 mg/L).
- Reported statistics and error estimates:
- no data
- Validity criteria fulfilled:
- yes
- Conclusions:
- Di-t-butyl peroxide to Daphnia magna was determined in a 48‑hour static test, under GLP and according to OECD guideline. The mean measured test concentration was 48.9% of the nominal value (i.e. 73.1 mg/L). In the control and at the mean measured test item concentration of 73.1 mg/L (nominal 150 mg/L), no immobilized test organisms were observed during the test period of 48 hours. Thus, EC50(72h) >73.1 mg/L and the No effect concentration >= 73.1 mg/L.
- Executive summary:
The acute toxicity of the test item di-t-butyl peroxide to Daphnia magna was determined in a 48‑hour static test according to the EU Commission Directive 92/69/, Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004), under GLP. A limit test was performed in accordance with the test guidelines to demonstrate that the test item has no toxic effect on the test organisms up to and including a nominal concentration of 150 mg/L. Thus, the only nominal concentration tested was 150 mg/L. Additionally, a control group was tested in parallel. The analytically determined test item concentration in the analyzed test medium was 126% of the nominal value (189 mg/L) at the start of the test. During the test period of 48 hours, a decrease of the test item concentration in the test medium was determined. At the end of the test, 19% of the nominal value (28 mg/L) was found, presumably due to loss to the atmosphere despite the use of closed vessels.
The mean measured test concentration was 48.9% of the nominal value. Therefore, the reported biological results are related to the mean measured test item concentration of 73.1 mg/L. In the control and at the mean measured test item concentration of 73.1 mg/L (nominal 150 mg/L), no immobilized test organisms were observed during the test period of 48 hours.
Reference
Table: Effect of di-t-butyl peroxide on the mobility of Daphnia magna.
Nominal |
No. of |
Immobilized |
Immobilized |
||
(mg/L) |
|
No. |
% |
No. |
% |
Control |
20 |
0 |
0 |
0 |
0 |
150 |
20 |
0 |
0 |
0 |
0 |
#: mean measured concentration
Description of key information
The geometric mean was used in place of nominal concentrations. Finally a 48h-EC50 > 73.1 mg/L was calculated. This was based on no observed effect at the highest concentration tested.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 73.1 mg/L
Additional information
A reliable GLP study on Daphnia magna following OECD 202 is available. No immobilization was observed in a limit test at 150 mg/L. However, analytical measurements showed concentration of the substance decreased in the medium from 126% at test initiation to 19% at test termination, presumably due to loss to the atmosphere despite the use of closed vessels. The geometric mean was used in place of nominal concentrations. Finally a 48h-EC50 > 73.1 mg/L was calculated. This is considered acceptable as test substance concentrations were still measurable at the end of the study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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