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EC number: 446-800-7 | CAS number: 175357-18-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline, GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2003-02-13
Test material
- Reference substance name:
- sepiwhite msh
- IUPAC Name:
- sepiwhite msh
- Details on test material:
- Sponsor's identification : SEPIWHITE MSH
Description : off-white powder
Batch number : 0214400005 (see Appendix 2)
Date received : 13 October 2003
Storage conditions : approximately 4ºC in the dark
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- A mixed population of activated sewage sludge micro-organisms was obtained on
16 February 2004 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at
Loughborough, Leicestershire, UK, which treats predominantly domestic sewage. - Duration of test (contact time):
- ca. 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 13.8 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 10 mg/L
- Based on:
- other: mg carbon /L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The CO2 produced by degradation was collected in two 500 ml Dreschel bottles containing 350 ml of 0.05 M NaOH. The CO2 absorbing solutions were prepared using purified de-gassed water.
Preparation of inoculum
The activated sewage sludge sample was maintained on continuous aeration in the laboratory at a
temperature of 21ºC and was used on the day of collection. Determination of the suspended
solids level of the activated sewage sludge was carried out by filtering a sample (100 ml) of the
activated sewage sludge by suction through pre-weighed GF/A filter paper using a Buchner
funnel. The filter paper was then dried in an oven at approximately 105ºC for at least 1 hour and
allowed to cool before weighing. This process was repeated until a constant weight was attained.
The suspended solids was equal to 2.8 g/l prior to use.
Culture medium
The culture medium used in this study (see Appendix 1) was that recommended in the
OECD Guidelines.
Preparation of test system
The following test preparations were prepared and inoculated in 5 litre glass culture vessels each
containing 3 litres of solution:
a) A control, in duplicate, consisting of inoculated culture medium.
b) The standard material (sodium benzoate), in duplicate, in inoculated culture medium to give a
final concentration of 10 mg carbon/l.
c) The test material, in duplicate, in inoculated culture medium to give a final concentration of
10 mg carbon/l.
d) The test material plus the standard material in inoculated culture medium to give a final
concentration of 20 mg carbon/l to act as a toxicity control (one vessel only).
Each test vessel was inoculated with the prepared inoculum at a final concentration of 30 mg
suspended solids (ss)/l. The study was carried out in a temperature controlled room at 21°C, in
darkness.
Approximately 24 hours prior to addition of the test and standard materials the vessels were filled
with 2400 ml of culture medium and 32.1 ml of inoculum and aerated overnight. On Day 0 the
test and standard materials were added and the volume in all the vessels adjusted to 3 litres by the
addition of culture medium.
The culture vessels were sealed and CO2-free air bubbled through the solution at a rate of
approximately 40 ml/minute and stirred continuously by magnetic stirrer.
The CO2-free air was produced by passing compressed air through a glass column containing selfindicating
soda lime (Carbosorb®) granules.
The CO2 produced by degradation was collected in two 500 ml Dreschel bottles containing
350 ml of 0.05 M NaOH. The CO2 absorbing solutions were prepared using purified de-gassed
water.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 75
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance):
4 % degradation after 1 d
22 % degradation after 2 d
44 % degradation after 6 d
67 % degradation after 8 d
72 % degradation after 10 d
75 % degradation after 28 d
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
16 % degradation after 1 d
41 % degradation after 2 d
63 % degradation after 6 d
66 % degradation after 8 d
71 % degradation after 10 d
85 % degradation after 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test material attained 75% degradation after 28 days and satisfied the 10-Day window
validation criterion, whereby 60% degradation must be attained within 10 days of the degradation
rate exceeding 10%. The test material can therefore be considered to be readily biodegradable
under the strict terms and conditions of OECD Guideline No 301B. - Executive summary:
Introduction.
A study was performed to assess the ready biodegradability of the test material in an aerobic aqueous medium. The method followed that described in the OECD Guidelines for Testing of Chemicals (1992) No 301B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC), and US EPA Fate, Transport, and Transformation Test Guidelines OPPTS 835.3110 Paragraph (m).
Methods.
The test material, at a concentration of 10 mg C/l, was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in the dark at 21°C for 28 days.
The degradation of the test material was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
Results.
The test material attained 75% degradation after 28 days and satisfied the 10-Day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation rate exceeding 10%. The test material can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301B.
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