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EC number: 208-293-9 | CAS number: 520-45-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Oral LD50 in rats 1480/1620 mg/kg (females/males); range of doses 1000 to 6400 mg/kg bw; significant toxicity at 2000 mg/kg.
Dermal LD50 in rabbits >3000 mg/kg; low dose 1000 mg/kg, top dose 5000 mg/kg; one animal died at 5000 mg/kg
.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study reported in 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- (only basic data given)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted Feb 1987
- Deviations:
- yes
- Remarks:
- (only basic data given)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Specific details on test material used for the study:
- Dehydroacetic acid
- Species:
- rat
- Strain:
- other: albino
- Remarks:
- 200-300 grams body weight
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Fasting period before study: 24 h
- Housing: 5 animals of the same sex per common cage
- Diet: ad libitum
- Water: ad libitum - Route of administration:
- oral: gavage
- Vehicle:
- propylene glycol
- Doses:
- 1000, 1600, 2000, 2500, 3200, 4000, 5000 and 6400 g/kg (males)
1000, 1250, 1600, 2000, 2500, 3200, 4000, 5000 and 6400 g/kg (females) - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: daily - Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 480 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 620 mg/kg bw
- Based on:
- test mat.
- Mortality:
- For details refer to Table 1 under "any other information on results"
- Clinical signs:
- other: All dose goups: lethargy 2000 mg/kg bw or more: gradual loss of motor control on the second day, magnified loss of motor control in further study progress, weight loss and debilitated survivors 5000 mg/kg or more: lack of motor control, hyperactive respo
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The LD50 values determined were 1620 mg/kg bw for the males and 1480 mg/kg bw for the females.
- Executive summary:
An acute oral toxicity study was conducted in male and female rats. Dosages in the range 1000 to 6400 mg/kg bw were tested. The LD50 values determined were 1620 mg/kg bw for the males and 1480 mg/kg bw for the females. Clinical signs noted included: lethargy, lack of motor control, hyperactive responses, convulsions and hemorrhaging from the nose and mouth.
Reference
Table 1: Acute oral toxicity
Dose | Mortality | Clinical signs |
[mg/kg bw] | ||
N* | N* | |
Males | ||
1000 | 0/5 | 5/5 |
1600 | 2/5 | 5/5 |
2000 | 4/5 | 5/5 |
2500 | 5/5 | 5/5 |
3200 | 5/5 | 5/5 |
4000 | 5/5 | 5/5 |
5000 | 5/5 | 5/5 |
6400 | 5/5 | 5/5 |
Females | ||
1000 | 0/5 | 5/5 |
1250 | 1/5 | 5/5 |
1600 | 3/5 | 5/5 |
2000 | 5/5 | 5/5 |
2500 | 5/5 | 5/5 |
3200 | 5/5 | 5/5 |
4000 | 5/5 | 5/5 |
5000 | 5/5 | 5/5 |
6400 | 5/5 | 5/5 |
*N = Number of animals
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 480 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- other justification
- Justification for data waiving:
- the study does not need to be conducted because exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size
- Justification for type of information:
- JUSTIFICATION FOR DATA WAIVING
Acute toxicity data are available for the oral and dermal routes. Examination of the life cycle of the substance indicates that human exposure is controlled by risk management measures in the industrial and professional settings. It is therefore expected that inhalation exposure from the identified uses will be low. In addition, the most likely route of exposure for workers and consumers is the dermal route, where lack of systemic toxicity has been experimentally demonstrated. Consumers will only be exposed to low concentrations of the substance in solutions, where inhalation exposure is unlikely.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Reported in 1950
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Method was described as essentially like that of Draize et al., (1944) J. Pharmacol. Exp. Therap. 82; 377. Cited in Principles and Methods of Toxicology, 5th Edition; ed. A. Wallace-Hayes, 2008.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rabbit
- Strain:
- not specified
- Sex:
- not specified
- Type of coverage:
- occlusive
- Vehicle:
- other: Greaseless base - "Neobase".
- Details on dermal exposure:
- After application of the dosage, the site was occluded by bandages for 24 hours after which time they were removed and the site washed with soap and water.
- Duration of exposure:
- 24 hours
- Doses:
- 1000, 3000 or 5000 mg/kg bw
- No. of animals per sex per dose:
- 2
- Control animals:
- no
- Sex:
- not specified
- Dose descriptor:
- approximate LD50
- Effect level:
- > 3 000 - < 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- One of the two rabbits treated at 5000 mg/kg bw died
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation(EC) No. 1272/2008
- Conclusions:
- No rabbits died at 1000 or 3000 mg/kg bw. The LD50 was considered to be greater than 3000 mg/kg bw
- Executive summary:
Rabbits were treated dermally for 24 hours with dihydroacetic acid at dosages between 1000 and 5000 mg/kg bw. There were no deaths at 1000 or 3000 mg/kg bw. The LD50 was considered to be greater than 3000 mg/kg bw.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 3 000 mg/kg bw
Additional information
Inhalation study waived because the study need not be conducted because exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size.
Justification for classification or non-classification
Oral: Category 4 based on CLP criteria
Dermal: Not classified according to CLP criteria, based on LD50 > 2000 mg/kg bw.
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