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Diss Factsheets
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EC number: 216-823-5 | CAS number: 1675-54-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- not specified
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to test guidelines and in accordance with GLP; however, there was no analytical confirmation of the test substance solutions reported in this study.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- No clean water control, only a solvent (acetone) control included in the test design
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- no
- Details on sampling:
- No analytical sampling conducted.
- Vehicle:
- yes
- Details on test solutions:
- Fresh test solutions were prepared daily by adding small quantities of stock solutions of Epikote 828 in acetone to 500 ml of water in clean beakers.
The total amount of acetone added to each beaker, including controls, was adjusted to 0.05 ml (0.1 ml/L).
Fresh stock solutions were prepared every 7 days.
The test solutions also contained quantities of algal cells (Chlorella vulgaris; 0.1 to 0.5 x 10^6 cells/ml), yeast (5 mg/L) and soil extract (20 ml/L), as food. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: D. magna
- Strain/clone: The culture was derived froma clone held at ICI Brixham Laboratory, supplied originally to ICI by IRChA, France.
- The D. magna used in the test were less than 24 hours old.
The D. magna are held in 0.5 to 4 liters of water in suitably sized glass vessels, at densities of up to 20 animals per liter. They are kept in a temperature controlled room (20 plus or minus 2 degrees C) under artificial cool white light regulated to give a light/dark cycle of 16h/8h. The cultures are changed to fresh water at least once per week. This is a reconstituted hard water (pH 7.0 - 8.5; hardness 160 to 220 mg/L as CaCO3), identical to that used for the toxicity tests. The cultures are fed daily with Chlorella vulgaris and active dried yeast. Typical feeding rates are 10^6 cells/mL of Chlorella and 5 mg/L of yeast. The cultures are sieved regularly to remove young. Adults are first removed temprarily by pipette and the culture vessel contents passed through a sieve (5.6 mm mesh) on which the young animals are retained. The water is returned, with the adult D. magna to the culture vessel.
Young for use in toxicity tests are less than 24 hours old and are colleted from cultures ages between 15 and 35 days. 24 h before a test is set up the culture is sieved and the young discarded. The culture is then re-sieved 23 h later. D. magna retained on the sieve are then left in clean water for 1 h (they are now up to 24 hours old) and those swiming actively are used for testing. Young for testing are not taken from cultures which contain ephippia; these cultures are discarded.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- no data
- Hardness:
- 160 - 180 mg/L CaCO3
- Test temperature:
- 18-22 degrees C
- pH:
- 7.6 - 8.5
- Dissolved oxygen:
- 7.1 - 9.1 mg/L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0, 0.03, 0.1, 0.3, 1, 3
- Details on test conditions:
- 21-day static test with daily renewal of the test solutions.
TEST SYSTEM
- Test vessel: 600 ml glass beakers
- Fill volume: 500 ml
- Renewal rate of test solution (frequency/flow rate): daily renewals of test solutions
- No. of organisms per vessel: 10 organisms/ vessel
- No. of vessels per concentration (replicates): 4 replicate beakers per treatment
- No. of vessels per control (replicates): 4 solvent control beakers
OTHER TEST CONDITIONS
- Test was carried out in a temperature-controlled laboratory at a nominal temperature of 20 plus or minus 2 degrees C.
- Photoperiod: 16h light/ 6 h dark
- Light intensity: white fluorescent lighting was used
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Every 24 hours
VEHICLE CONTROL PERFORMED: yes, but no clean water control
RANGE-FINDING STUDY
- Test concentrations: 0.1 - 10 mg/L
- Results used to determine the conditions for the definitive study: 48 hour EC50 = 1.7 mg/L - Reference substance (positive control):
- not specified
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: survival, growth and reproduction
- Remarks on result:
- other: no data
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: survival, growth and reproduction
- Remarks on result:
- other: no data
- Details on results:
- All the D. magna exposed to 3 mg/L of the test material died within 48 hours. Significant mortalities also occurred at 1 mg/L, accounting for 35% of the original animals by the end of the test. Mortalities in the other treatments, however, were no more than 5% and not statistically significant. Growth was also significantly reduced at 1 mg/L, the adults still alive at the end of the test being on average 7% smaller than in the controls. Reproductive output was significantly reduced at 1 mg/L. Although the onset of reproduction was no later than in the controls, the average number of young produced per adult over the test as a whole was some 20% less.
No effects were observed at a concentration of 0.3 mg/L but survival, growth and reproduction were all significantly reduced at 1 mg/L. - Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- no data
- Validity criteria fulfilled:
- yes
- Remarks:
- One control beaker was omitted from the anaysis as it showed lower than normal reproduction.
- Conclusions:
- The NOEC is 0.3 mg/L but survival, growth and reproduction were all significantly reduced at 1 mg/L.
- Executive summary:
D. magna were exposed to five concentrations of Epikote 828 ranging from 0.03 - 3 mg/L and control dilution water for 21 days in a static renewal test design. Survival, growth, and reproduction rate were all significantly reduced at 1 mg/L of Epikote 828, but were all unaffected at 0.3 mg/L.
Reference
In the chronic toxicity test, survival, growth and reproduction rate were all significantly reduced at 1 mg/L of EPIKOTE 828, but were all unaffected at 0.3 mg/L. The threshold concentration for chronic toxic effect thus appears to lie somewhere between 0.3 and 1 mg/L, and therefore to be no more than about 5 times below the 48 h EC50 value. On this basis it is concluded that EPIKOTE 828 has a relatively low potential for chronic harm to D. magna beyond its acute toxicity.
Description of key information
The 21-day NOEC of 0.3 mg/L for BADGE and BADGE-related resins is based on surivival, growth and reproduction of Daphnia magna in a chronic toxicity test with BADGE.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.3 mg/L
Additional information
One long term test with Daphnia magna was conducted with BADGE. This test was deemed reliable and useful for risk assessment (Klimish score = 2). In this test, D. magna were exposed to five concentrations of Epikote 828 ranging from 0.03 - 3 mg/L and control dilution water for 21 days in a static renewal test design. Survival, growth, and reproduction rate were all significantly reduced at 1 mg/L of Epikote 828, but were all unaffected at 0.3 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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