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Diss Factsheets
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EC number: 920-008-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- Sept. 6, 1996-Dec. 9, 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sample storage conditions before analysis: If samples could not be analysed the day they were taken, they were stored at 4°C and extracted at the earliest opportunity. Samples were stable for up to 7 days. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Water accomodated fractions of Shellsol H were produced by stirring water appropriate to the test organisms and Shellsol H for 45 hours, leaving the contents of the vessel to stand at least for 1 hour and running off the aqueous phase for use as the test medium. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Strauss
- Source: clonal laboratory culture derived from animals obtained from Zeneca Brixham Laboratory
- Age at study initiation (mean and range, SD): less than 24 hrs
- Method of breeding: Cultures started with animals less than 24 hours old, and discarded when 28 days old. Young are removed daily, and animals for testing removed 23 hours after last removal. Test young are from 15-28 day cultures from at least the third brood. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 156-190 mg/l CaCO3
- Test temperature:
- 19-20°C
- pH:
- 7.9-8.5
- Dissolved oxygen:
- 8.4-10.8
- Salinity:
- Not applicable (freswater test)
- Nominal and measured concentrations:
- Nominal loading rate: 0, 10, 22, 46, 100, 220, 460, 1000 g/l.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sealed with a screw cap
- Material, size, headspace, fill volume: 150 ml Erlenmeyer flasks completely filled with WAF or dilution water (control)
- Renewal rate of test solution (frequency/flow rate): static
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The water comes from two pumping stations (Highstead and controlled by the Mid Kent Water Company. The water is obtained from bore holes in the chalk of the North Downs. The only chemical treatment prior to its arrival in the laboratory is chlorination to 0.1 mg/l. In the laboratory, the water is filtered (PAL MDY 1001 Y400) to remove all particles larger than 15 µm (90% of particles greater than 10µm) and passed through activated carbon filters (Cuno model CT) to remove chlorine and organic conatminants. Both particle and activated carbon filter cartridges are renewed as recommended by the manufacturers. Heat exchange units (stainless steel and perspex) are used to adjust the temperature of the water.
- Intervals of water quality measurement: six months
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization
. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 100 - 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- 460 - 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hr EL50 range for an invertebrate, Daphnia magna, was 100-220 mg/L (WAF).
- Executive summary:
The acute toxicity of water accomodated fractions (aqueous extrats) of Shellsol H has been determined to the crustacean zzoplakter Daphnia magna.
The test media were prepared by adding quantities of Shellsol H to culture medium appropriate to the test organisms and stirring in sealed vessels for 45 hours. The strirring period was demonstrated as being sufficient to ensure equilibration of the shellsol H and aqueous phases. After stirring the contents of the test vessels were left to settle for about 1 hour before drawing off the WAF for use as the test media in the toxicity tests.
The concentrations of dissolved hydrocarbons (derived from the shellsol H) in the test media was determined during the toxicit tests usig gas chromatography. The analyses showed that the concentration in the test media was <1%% of the loading rate. The reduction in the concentration of dissolved hydrocarbons in the test media during the test was ≤26%.
The acute toxicity to D. magna of water accomodated fractions of Shellsol H prepared at loading rates ranging from 10 to 1000 mg/l was determined in a 48 static toxicity test without renewal of the test media. The test was conducted in sealed test vessels. The 24 and 48 h EL50 values (loading rates resulting in mortality of 50% of the D. magna after 24 and 48 h exposure) were 460-1000 and 100 -220 mg/l respectively.
Reference
Description of key information
There is no data available for this substance. However, key data is available for the structural analogue Hydrocarbons, C10-C13, n-alkanes, Isoalkanes, cyclics, aromatics (2-25%). The data for this substance is presented in this dossier. The data is read across to these substances based on analogue read across and a discussion and report on the read across strategy is provided as an attachment in IUCLID Section 13.
Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) presented a 48-h LC50 (mortality) range forDaphnia magnaof 100 -220 mg/L (based on water accommodated fractions).
Key value for chemical safety assessment
Additional information
The study from Shell (1995) examined the toxicity of Shellsol H (Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%)) to the aquatic invertebrate Daphnia magna. The test media were prepared by adding quantities of Shellsol H to culture medium appropriate to the test organisms and stirring in sealed vessels for 45 hours. The strirring period was demonstrated as being sufficient to ensure equilibration of the shellsol H and aqueous phases. After stirring the contents of the test vessels were left to settle for about 1 hour before drawing off the WAF for use as the test media in the toxicity tests. The concentrations of dissolved hydrocarbons (derived from the shellsol H) in the test media was determined during the toxicit tests usig gas chromatography. The analyses showed that the concentration in the test media was <1% of the loading rate. The reduction in the concentration of dissolved hydrocarbons in the test media during the test was ≤26%. The acute toxicity to D. magna of water accomodated fractions of Shellsol H prepared at loading rates ranging from 10 to 1000 mg/l was determined in a 48 static toxicity test without renewal of the test media. The test was conducted in sealed test vessels. The 24 and 48 h EL50 values (loading rates resulting in mortality of 50% of the D. magna after 24 and 48 h exposure) were 460-1000 and 100 -220 mg/l respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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