Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-778-8 | CAS number: 695-06-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- not specified
- Details on test solutions:
- Dilution water
Top prepare the dilution water, 4 vials (1, 2, 3, 4) of different concentrated salts “ISO medium" (purchased from ECOTOX LOS), were used; each vial contains a concentrated salt solution enough to prepare 2 I of dilution water. The composition of each salt solution is given below.
Batch used: ISOD140617
Expiration date: 14/06/2019
NRI: 171VIT/2018 and 214VIT/2018
Vial 1: NaHC03 129.5 mg - dissolved in 2 I - final concentration 64. 75 mg/I
Vial 2: CaCl2’ 2H20 588 mg - dissolved in 2 I - final concentration 294 mg/I
Vial 3: MgS04 · 7H20 264.5 mg - dissolved in 2 I - final concentration 123.25 mg/I
Vial 4: KCI 11.5 mg - dissolved in 2 I - final concentration 5. 75 mg/I
The dilution water was prepared as follows:
A volumetric flask (2 I capacity) was filled with approximately 1 l of distilled water;
The content of each vial (from vial 1 to 4) was poured in the volumetric flask;
The flask was filled up to 2 I with distilled water and mixed.
The dilution water was transferred in dark glass containers and stored in the refrigerator (5°C+-3 °C), in the dark, for no longer than one month.
The pH of the dilution water was in the acceptable range of 7.8+-0.5. Before using the dilution water for the hatching of ephippia or for the p「eparation of the test solutions, it was g「adually brought to room temperature, and it was aerated for 30 minutes.
At the beginning of each test, pH and dissolved oxygen of the dilution water were measured. Dissolved oxygen was >7 mg/I (minimum acceptable value).
The compliance of pH and dissolved oxygen values was reported in the raw data of the Study.
K2Cr2O7
The sensitivity to potassium dichromate (K2Cr2O7) of the batch of ephippia employed in the assay, as indicated in the certificate, was assessed preliminarily The results are reported in the control chart Mod. 775/GxP.
Reagents for TOC analysis
Sucrose
1,4-Benzoquinone
Nitric acid concentrate 70% (for washing volumetric flasks employed in the Tl-0016031 analysis)
Sucrose solutions were freshly prepared prior to begin with the analysis at each time point.
At first the sucrose was heated in a oven for at least 3 hours at 105 °C and afterwards dried for at least 1 hour. Then a 5000 ppm working solution (RSO) was prepared by solving the sucrose in MilliQ H20.
From the RSO solution, 1 ml was transferred in a new 100 ml volumetric flask and brought to volume with MillliQ H20 in order to prepared the RS0-50 ppm solution.
The other dilutions were prepared as follows:
RS (0.2 ppm) = 0.4 ml of RS0-50 ppm were transferred into a 100 ml volumetric flask and brought to volume with milliQ H20;
RS2 (1 ppm)= 2 ml of RS0-50 ppm were transferred into a 100 ml volumetric flask and brought to volume with milliQ H20;
RS3 (2.5 ppm) = 5 ml of RS0-50 ppm were transferred into a 100 ml volumetric flask and brought to volume with milliQ H20;
RS4 (5 ppm)= 10 ml of RS0-50 ppm were transferred into a 100 ml volumetric flask and brought to volume with milliQ H20;
RS5 (1 O ppm) = 20 ml of RS0-50 ppm were transferred into a 100 ml volumetric flask and brought to volume with milliQ H20;
RS6 {20 ppm} = 40 ml of RS0-50 ppm were transferred into a 100 ml volumetric flask and brought to volume with milliQ H20.
A 5000 ppm 1.4-Benzoquinone stock solution (BQO-5000 ppm) was prepared in MilliQ H20 and the solution was then sonicated for 10 minutes.
Afterwards BQ1 (50 ppm) solution was prepared by transferring 1 ml of BQO into a new 100 ml volumetric flask and bringing to volume with MilliQ H20.
Finally BQ2 (0.5 ppm) solution was prepared by transferring 1 ml of 801 into a new 100 ml volumetric flask and flask and bringing to volume with MilliQ H20. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- As test system Daphnia magna Straus (Cladocera, Crustacea) was employed in the assay, as described by the guideline OECD 202:2004. The animals were less than 24 hours old at the start of the test, and they were not first brood progeny. They derived from Daρhnia magna ephippia, supplied by the qualified provider ECOTOX LDS. Ephippia are a quiescent form of animals which can be hatched when needed.
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 21°C
- pH:
- 7.38-7.70
- Dissolved oxygen:
- >3mg/L
- Nominal and measured concentrations:
- The definitive test was carried out, since in the preliminary assay the test substance at the concentration 100 mg/I induced > 10% animal immobilization. The definitive test was carried out on 5 concentrations (from C1to C5) which, on the basis of the preliminary test results, allowed the EC50 calculation. The selected nominal concentrations were:
C1: 25 mg/I
C2: 12.5 mg/I
C3: 6.25 mg/I
C4: 3.125 mg/I
CS: 1.562 mg/I - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 3.176 mg/L
- Nominal / measured:
- nominal
- Details on results:
- At the end of the definitive test, EC50 was calculated using the EPA software PROBIT, 95%confidence limits were also calculated. The PROBIT elaboration is attached to the raw data. EC50: 3.176 mg/I (confidence limits 2.478 mg/I-4.001 mg/I).
The graph of the fitted model used for the calculation of the EC50 is attached to the raw data. - Results with reference substance (positive control):
- Prior to routine use of the batch of Daphnia magna, the sensitivity toward K2Cr2O7 was verified by carrying out a
test with 5 concentrations of this chemical; the concentrations used in this test were: 3.2 mg/I - 1.8 mg/I - 1 mg/I -
0.56 mg/I -0.32 mg/I. Results of the tests were reported in the control chart 775/GxP. The calculated ECso resulted
between 0.6 mg/1 and 2.1 mg/I. - Validity criteria fulfilled:
- yes
- Conclusions:
- According to OECD 202:2004, under the test conditions applied, the EC50 of the test substance is calculated as 3.176 mg/L.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 3.176 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)