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EC number: 203-691-9 | CAS number: 109-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24/11/97 to 16/12/97
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- No further details specified in the study report.
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical chemistry and physico-chemical measurements were carried out at the beginning and the end of the test.
- Vehicle:
- not specified
- Details on test solutions:
- Not specified
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Biological reagent: Test organisms are freshwater algae, green unicellular, Pseudokirchneriel! a subcapitata strain CCAP 278/4 (previously named Raphidocelis subcapitata and Selenastrum capricornutum); the strain used was obtained from the Culture Center of Algae and Protozoa.
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not specified
- Hardness:
- Not specified
- Test temperature:
- 23.4 ± 0.9 °C
- pH:
- During the test, the control pH varied by 0.45 units.
- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable.
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- The effect concentrations and NOEC have been calculated using the initial concentrations of n-BUTYL BROMIDE determined by gas chromatography according to the method described in annex to this report.
- Details on test conditions:
- The study was performed using 120 ml glass bottles stoppered with PTFE bungs and sealed with aluminium caps, containing 50 ml of test solution inoculated with an algal suspension so that the initial cell concentration was equal to 1 x 104 cells/ml. Tests flasks were incubated at 23.4 ± 0.9 °C continuously shaken and constantly exposed to a light intensity of between 6000 and 6900 lux. The algal concentration was measured daily.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.93 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass & growth rate
- Details on results:
- The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear, colourless. No precipitation was observed at the end of the test.
Microscopic observation confirmed that the algae appeared deformed, turgid in the 3 highest concentrations in comparison to the control: the normal form of the unicellular algae is a crescent shaped cell with an average length of 5-10 μm. - Results with reference substance (positive control):
- Not specified
- Reported statistics and error estimates:
- The No Observed Effect Concentration (NOEC), corresponding to the highest tested concentration where no significant effect was observed in comparison to the control, was determined using a statistical analytical method (Dunnett test).
- Validity criteria fulfilled:
- yes
- Conclusions:
- EbC50: 6.9 mg/l
ErC50: 9.98 mg/l
The No Observed Effect Concentration (NOEC): 1.93 mg/l - Executive summary:
The determination of the growth inhibition (chronic toxicity) of the freshwater algae Pseudokirchneriella subcapitata (previously known as Raphidocelis subcapitata and Selenastrum capricornutum) exposed to the test substance n-BUTYL BROMIDE (BROMURE DE n-BUTYLE) for a duration of 72 hours was performed following the method C3 described in Directive 92/69/EEC of the European Commission and in the guideline 201 of the OECD.
Algae were exposed to a range of concentrations of n-BUTYL BROMIDE dissolved in dilution water. The toxic effect measured during the assay was the inhibition of cellular multiplication over a time period of 72 hours.
The study was performed using 120 ml glass bottles stoppered with PTFE bungs and sealed with aluminium caps, containing 50 ml of test solution inoculated with an algal suspension so that the initial cell concentration was equal to 1 x 104cells/ml. Tests flasks were incubated at 23.4 ± 0.9 °C continuously shaken and constantly exposed to a light intensity of between 6000 and 6900 lux. The algal concentration was measured daily.
Analytical chemistry and physico-chemical measurements were carried out at the beginning and the end of the test.
The concentration of test substance causing a 50 % reduction in biomass (EbC50) or in growth rate (ErC50) was determined. The No Observed Effect Concentration (NOEC), corresponding to the highest tested concentration where no significant effect was observed in comparison to the control, was determined using a statistical analytical method (Dunnett test). The results were as follows:
Effect concentration and NOEC (mg/l)
Value
95 % CI
Biomass
EbC50 – 72h
6.9
(4.6 – 9.6)
EbC10 – 72h
2.9
(1.4 – 4.3)
NOECb
1.93
ND
Growth rate
ErC50 – 72h
9.9
(7.8 – 13)
ErC10 – 72h
4.1
(2.9 – 5.4)
NOECr
1.93
ND
CI: 95 % confident interval
ND: not determined
The effect concentrations and NOEC shown in the previous table have been calculated using the initial concentrations of n-BUTYL BROMIDE determined by gas chromatography according to the method described in annex to this report.
The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear, colourless. No precipitation was observed at the end of the test.
Microscopic observation confirmed that the algae appeared deformed, turgid in the 3 highest concentrations in comparison to the control: the normal form of the unicellular algae is a crescent shaped cell with an average length of 5-10 μm.
During the test, the control pH varied by 0.45 units.
The validity criteria of the study were respected: the increase in cell density (R), measured in the control solution between the end and the beginning of the test, was greater than a factor of 16 (R =57); the final concentrations of n-BUTYL BROMIDE were maintained within the designated limit of 80 % of the initial concentration in non-inoculated flasks.
The study was performed according to the principles of Good Laboratory Practice (GLP) as described by OECD.
Reference
Description of key information
EbC50: 6.9 mg/l
ErC50: 9.98 mg/l
The No Observed Effect Concentration (NOEC): 1.93 mg/l
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 9.9 mg/L
- EC10 or NOEC for freshwater algae:
- 1.93 mg/L
Additional information
The determination of the growth inhibition (chronic toxicity) of the freshwater algae Pseudokirchneriella subcapitata (previously known as Raphidocelis subcapitata and Selenastrum capricornutum) exposed to the test substance n-BUTYL BROMIDE (BROMURE DE n-BUTYLE) for a duration of 72 hours was performed following the method C3 described in Directive 92/69/EEC of the European Commission and in the guideline 201 of the OECD.
Algae were exposed to a range of concentrations of n-BUTYL BROMIDE dissolved in dilution water. The toxic effect measured during the assay was the inhibition of cellular multiplication over a time period of 72 hours.
The study was performed using 120 ml glass bottles stoppered with PTFE bungs and sealed with aluminium caps, containing 50 ml of test solution inoculated with an algal suspension so that the initial cell concentration was equal to 1 x 104cells/ml. Tests flasks were incubated at 23.4 ± 0.9 °C continuously shaken and constantly exposed to a light intensity of between 6000 and 6900 lux. The algal concentration was measured daily.
Analytical chemistry and physico-chemical measurements were carried out at the beginning and the end of the test.
The concentration of test substance causing a 50 % reduction in biomass (EbC50) or in growth rate (ErC50) was determined. The No Observed Effect Concentration (NOEC), corresponding to the highest tested concentration where no significant effect was observed in comparison to the control, was determined using a statistical analytical method (Dunnett test). The results were as follows:
Effect concentration and NOEC (mg/l) |
||
|
Value |
95 % CI |
Biomass |
||
EbC50 – 72h |
6.9 |
(4.6 – 9.6) |
EbC10 – 72h |
2.9 |
(1.4 – 4.3) |
NOECb |
1.93 |
ND |
Growth rate |
||
ErC50 – 72h |
9.9 |
(7.8 – 13) |
ErC10 – 72h |
4.1 |
(2.9 – 5.4) |
NOECr |
1.93 |
ND |
CI: 95 % confident interval
ND: not determined
The effect concentrations and NOEC shown in the previous table have been calculated using the initial concentrations of n-BUTYL BROMIDE determined by gas chromatography according to the method described in annex to this report.
The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear, colourless. No precipitation was observed at the end of the test.
Microscopic observation confirmed that the algae appeared deformed, turgid in the 3 highest concentrations in comparison to the control: the normal form of the unicellular algae is a crescent shaped cell with an average length of 5-10 μm.
During the test, the control pH varied by 0.45 units.
The validity criteria of the study were respected: the increase in cell density (R), measured in the control solution between the end and the beginning of the test, was greater than a factor of 16 (R =57); the final concentrations of n-BUTYL BROMIDE were maintained within the designated limit of 80 % of the initial concentration in non-inoculated flasks.
The study was performed according to the principles of Good Laboratory Practice (GLP) as described by OECD.
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