4-aminobutyric acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guideline and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-aminobutyric acid

EC Number:

200-258-6

EC Name:

4-aminobutyric acid

Cas Number:

56-12-2

Molecular formula:

C4H9NO2

IUPAC Name:

4-aminobutanoic acid

Test material form:

solid: crystalline

Details on test material:

Test item: 4-Aminobutyric acid
CAS number: 56-12-2
Batch number BCBN4740V
Physical state: Solid, crystalline powder
Colour: White
Storage: at 15-30 °C

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Type of inoculum: aerobic mixed bacterial culture
Source: Municipal wastewater treatment plant, Esztergom-Kertváros

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

PREPARATION OF INOCULUM TEST CULTURE
Before the test 300 ml stock culture was filtered through a filter with 0.2 mm pore size to remove the bigger insoluble particles and then the dry substance content of the filtrate was determined. The measured value was 0.78 g/L. As the dry substance was less than 1 g/l, the filtrate was used as inoculum culture directly.

PREPARATION OF REFERENCE SOLUTION
For the reference solution 320.12 mg sodium acetate was diluted in 100 ml mineral medium.

PREPARATION OF TEST VESSELS
Following test vessels were prepared and analysed for the study:
- Test item (in two replicates)
- Blank control (in two replicates)
- Reference solution (positive control)
- Reference and test item solution (toxicity control)
- Sterilized solution of test item (abiotic control)

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

80

Sampling time:

28 d

Remarks on result:

other: mean of two replicates

Details on results:

The biodegradability of the test item on day 12th was 74%. The abiotic degradation of the test item was 1% at the end of the test.

BOD5 / COD results

Results with reference substance:

Reference solution (positive control): 85%
Reference and test item solution (toxicity control): 68%
Sterilized solution of test item (abiotic control): 1%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

According to OECD guideline the test item should be classified as readily biodegradable if the percentage biodegradation reaches 60% of theoretical oxygen demand within 28 day period in a 10-day window. Based on ready biodegradability testing results the 4-Aminobutyric acid is readily biodegradable.