Methacrylamide

Carcinogenicity

Administrative data

Endpoint:

carcinogenicity

Remarks:

intraperitoneal

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Peer reviewed in OECD SIDS. No GLP. Dose dependency was not clear because methacrylamide was administered at one dose level. Descriptions about animal husbandry including barrier system were not reported. Therefore the reliability of this study was considered limited.

Data source

Reference

Reference Type:

publication

Title:

Studium der Lungentumorbildung bei Mäusen durch Methacrylamid.

Author:

Matsuda H

Year:

1989

Bibliographic source:

Kanazawa Daigaku Juzen Igakkai Zasshi 98(2): 428-438; translated on behalf of Röhm GmbH

Materials and methods

Principles of method if other than guideline:

Method: no data

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methacrylamide

EC Number:

201-202-3

EC Name:

Methacrylamide

Cas Number:

79-39-0

Molecular formula:

C4H7NO

IUPAC Name:

methacrylamide

Test material form:

solid

Specific details on test material used for the study:

Purity of the test substance: no data

Test animals

Species:

mouse

Strain:

other: ddY

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Duration of treatment / exposure:

5 days

Frequency of treatment:

daily (first group) or 5 times every second day (second group)

Doses / concentrations

Remarks:

Doses / Concentrations:
200 mg/kg in aqueous solution
Basis:

Control animals:

yes

Details on study design:

Post-exposure period: 6 months

Results and discussion

Any other information on results incl. tables

Results:
In the first dose group in 16 of 57 (28%) mice and in the second dose group in 
8 of 38 (21%) mice, an increased number of lung adenoma were found. Control 
(1 out of 48 (2.1%) lung tumor formation was found. 
After 3 hours it was shown with radiolabelled 14C-methacrylamide that the highest
concentrations of methacrylamide were found in the kidneys, followed by liver, 
blood, spleen and lungs. Approx. 5 % of the radiolabelled methacrylamide in the
lung was bound to the protein fraction. A direct alkylation of nucleosides in 
vitro was not observed. The lipid peroxidation of the microsomes was neither 
affected in vitro nor in vivo.
Histopathology of tumors: All tumors were identified as adenoma resulting from 
alveolar type II epithelial cells.
Control group: After 6 months, 1 of 48 animals had a tumor.

Dose dependency was not clear because methacrylamide was administered at one 
dose level. Descriptions about animal husbandry including barrier system were 
not reported. Therefore the reliability of this study was considered limited.