Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-618-5 | CAS number: 85-60-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 July 1980 to 20 July 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed following the intent of GLP with recognised standards referenced in the report.
- Qualifier:
- according to guideline
- Guideline:
- other: Committee on Methods for Toxicity Tests with Aquatic Organisms (C. E. Stephan, Chairman). 1975. Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. Environmental Protection Agency, Ecological Research Series EPA-660/3-75-009
- Qualifier:
- according to guideline
- Guideline:
- other: American Public Health Association. 1975. Standard Methods for the Examination of Water and Wastewater. 14th ed. Washington, DC. 1193 p.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not specified - Analytical monitoring:
- not specified
- Details on sampling:
- Based on the results of preliminary testing, five concentrations of the test compound, ranging in a logarithmic series from 100 to 1,000 mg/l.
The test concentrations were obtained by transferring appropriate weights of the sample directly to the test chambers.
The sample was stored at 4°C. - Vehicle:
- not specified
- Details on test solutions:
- Test concentrations were prepared based on the total compound. Before the test concentrations were prepared, the standard was allowed to warm to room temperature (22°C). The test concentrations were obtained by transferring appropriate weights of the sample directly to the test chambers. The compound was noted to precipitate in all test solutions .
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- The rainbow trout used in the test were obtained from Trout Lodge in McMillan, Washington. The fish were identified to species using the taxonomic keys developed by Eddy. All test fish were held in culture tanks on a 16 hour daylight photoperiod and observed for at least fourteen days prior to testing. Fish culture techniques used were basically those described by Brauhn et. al. A daily record of fish observations during the holding period, along with prophylactic and therapeutic disease treatments. During this period, the fish received a standard commercial fish food (Rangen's) daily until 48 hours prior to testing at which time feeding was discontinued. The rainbow trout used for this experiment had a mean weight of 0.35 g and a mean standard length of 29 mm. Weight and length measurements were made on the control group of fish at the termination of the test.
The test fish were acclimated to the dilution water and test temperature and held without food 48 hours prior to testing. - Test type:
- static
- Water media type:
- brackish water
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post exposure observation period specified in the report
- Hardness:
- 255ppm
- Test temperature:
- 12°C
- pH:
- 8.2
- Dissolved oxygen:
- 9.3ppm
- Salinity:
- Not specified
- Nominal and measured concentrations:
- Measured concentrations
- Details on test conditions:
- The static fish bioassay was conducted in five gallon glass vessels containing 15 litres of laboratory well water. These vessels were kept in a water bath at 12°C (±1.0). The test fish were acclimated to the dilution water and test temperature and held without food 48 hours prior to testing.
A 48 hour range-finding test was conducted to determine the concentration range for the definitive study. The preliminary test concentrations were set at 1,10 and 100 mg/l. Based on the results of preliminary testing, five concentrations of the test compound, ranging in a logarithmic series from 100 to 1,000 mg/l, with ten fish per concentration were selected for definitive bioassay, as approved by the study sponsor. The fish were added to the test chambers by random assignment within 30 minutes after addition of toxicant aliquots. - Reference substance (positive control):
- yes
- Remarks:
- Antimyin A
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I.
- Details on results:
- The dissolved oxygen concentration which stayed between 60 and 100% saturation was considered adequate for testing. The pH values remained consistent with the control throughout the study. The ammonia concentrations were below the toxic limit.
- Results with reference substance (positive control):
- As a quality check, the rainbow trout were challenged with a reference compound, Antimycin A. The observed 96 hour LCs0 and 95% confidence limits (C.I.) were within the 95% confidence limits reported in the literature, indicating that the fish were in good condition.
- Reported statistics and error estimates:
- Not specified
- Sublethal observations / clinical signs:
The Acute Toxicity of Santowhite Powder and Antimycin A to Rainbow Trout (Salmo gairdneri)*
LC50 in milligrams/litre (ppm)
Compound
24 hours
48 hours
96 hours
Santowhite powder
>1,000
>1,000
>1,000
Antimycin A
0.000060
(0.000042 – 0.000075)**
0.000028
(0.000023 – 0.000036)
0.000019
(0.000016 – 0.000024)
*Bioassay as conducted as 12°C (±1.0), mean weight and length, 0.35g and 29mm.
**95% confidence interval.
***Antimycin A standard obtained from Sigma Chemical Company, Type III, crystalline, Lot 125C – 0152.
Mortality Rates and Water Quality Measurements During the Acute Toxicity Test of Santowhite Powder to Rainbow Trout (Salmo gairdneri)
Water Quality
Percent Mortality Hours
0 hours
48 hours
96 hours
mg/l Concentration
24
48
96
Temp.°C
D.O.* mg/l
pH**
NH3*** mg/l
Temp.°C
D.O.* mg/l
pH**
NH3*** mg/l
Temp.°C
D.O.* mg/l
pH**
NH3*** mg/l
Control
0
0
0
12
8.3
7.6
0.18
12
8.0
7.6
0.30
12
8.5
7.7
0.26
100
0
0
0
12
8.7
7.5
0.20
12
8.5
7.7
0.26
12
7.7
7.8
0.18
180
0
0
0
320
0
0
0
560
0
0
0
1,000
0
0
0
12
8.9
7.5
0.21
12
8.3
7.7
0.24
12
7.5
7.7
0.20
*Dissolved oxygen concentrations – Dissolved Oxygen Probe (YSI Model 54).
**pH – pH Probe (Orion Model 91 – 06) used with an Extech Model 671 pH and mV meter.
***Total ammonia concentrations – ammonia Probe (Extech Model 8002 – 8) used with an Extech Model 671 pH and mV meter.
- Conclusions:
- 96 hour LC50 >1,000 mg/l
- Executive summary:
The acute toxicity of Santowhite Powder to rainbow trout (Salmo gairdneri) was assessed using the methods outlined by the Committee on Methods for Toxicity Tests with Aquatic Organisms. Water quality parameters of temperature, dissolved oxygen, pH and ammonia were measured throughout the test and were within acceptable limits. The study was conducted following the intent of the Good Laboratory Practice Regulations.
As a quality check, the rainbow trout were challenged with a reference compound, AntimycinA.The observed 96 hour LCs0 and 95% confidence limits (C.I.) were within the 95% confidence limits reported in the literature, indicating that the fish were in good condition.
The results of the four day static fish toxicity study using rainbow trout are summarized below:
Compound: Santowhite powder, 96 hour LC50 >1,000 mg/l
Compound: Antimycin A, 96 hour LC50 0.000019
Reference
Description of key information
Key value determined using US EPA and American Public Health Association guidance.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
Both the supporting studies provide the same result as the key value described.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.