3-nitro-p-anisic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed study but without pproper documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: UBA test proposal "Letale Wirkung beim Zebrabärbling Brachydanio rerio" dated 1982-06-01

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-nitro-p-anisic acid

EC Number:

201-906-0

EC Name:

3-nitro-p-anisic acid

Cas Number:

89-41-8

Molecular formula:

C8H7NO5

IUPAC Name:

4-methoxy-3-nitrobenzoic acid

Test solutions

Details on test solutions:

For the preparation of the stock solution 1 g of the substance were pre-solved in 1 g ethanol

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

length: 30 +-5 mm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

15 ° dH

Test temperature:

23 +- 2 °C

pH:

7.0 +- 0.2

Details on test conditions:

vessel : 8.4 l
test duration: 96 hours, no feeding during that time
aeration: 3 bubbles per second
10 animals per 3.5 l of water

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 is greater than 100 mg/L.

Executive summary:

The substance was tested for toxicity to Brachydanio rerio according to the procedure proposed by the German Umweltbundesamt with the title "Letale Wirkung beim Zebrabärbling Brachydanio rerio" dated 1982 -06 -01. In a limit test 100 mg/l were administered to ten fish. After 96 hours no letality was observed. The LC50 has therefore to be considered as geater than 100 mg/L