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Diss Factsheets
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EC number: 202-498-7 | CAS number: 96-31-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
- Reference Type:
- other: recalculation
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part 9
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,3-dimethylurea
- EC Number:
- 202-498-7
- EC Name:
- 1,3-dimethylurea
- Cas Number:
- 96-31-1
- Molecular formula:
- C3H8N2O
- IUPAC Name:
- 1,3-dimethylurea
- Details on test material:
- - Name of test material (as cited in study report): Dimethylharnstoff (1,3-dimethylurea)
- Analytical purity: no data given
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Scenedesmus subspicatus CHODAT
- Strain: SAG 86.81
- Source: Algae collection, University of Goettingen, Germany
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 20 °C
- pH:
- 8.5 - 8.6
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
0 (Control)
31.25
62.5
125
250
500 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Fill volume: 10 ml
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
GROWTH MEDIUM
- Standard medium used: according to OECD medium
TEST MEDIUM / WATER PARAMETERS
- Preparation of the medium: The test medium of the highest test concentration, 500 mg/l (nominal) was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. Stock solution: 1000 mg/l
OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light - light intensity: 50 - 200 uE at a wave length of 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: At 0, 24, 48, 72, and 96 hours after test start, the algae cell density was determined by spectro-fluorometrical measurement using an impulse fluorometer. The parameters pH and temperature were determined at the end of the test. Also, at the end of the incubation period the algae cells were analyzed for signs of photosynthesis blockage by means of a spectrophotometrical scan at 300 - 780 nm.
- Chlorophyll measurement: spontaneous chlorophyll-A fluorescence at 685 nm as criterion for biomass (excitation with short light impulse at 435 nm). Measurements at 0, 24, 48, and 72 hours.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 288.3 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Remarks on result:
- other: 187 - 387 mg/l 95% CL
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 678.53 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Remarks on result:
- other: 484 - 1592 mg/l 95% CL
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Remarks on result:
- other: not determined due to mathematical reasons
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 62.5 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC0
- Effect conc.:
- 99 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks:
- integral
- Remarks on result:
- other: 36 - 150 mg/l 95% CL
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 193 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks:
- integral
- Remarks on result:
- other: 115 - 258 mg/l 95% CL
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 688 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks:
- integral
- Remarks on result:
- other: 479 - 1555 mg/l 95% CL
- Details on results:
- Yield 72 h
EyC10: 51 (27 - 74 95% CL) mg/l
EyC20: 117 (83 - 146 95% CL) mg/l
EyC50: 580 (479 - 775 95% CL) mg/l
LOEyC: 125 mg/l
NOEyC: 62.5 mg/l
Original effect values as given in the report:
(nominal concentrations):
72 h-EC20 = 121.5 mg/l - Reported statistics and error estimates:
- Linear regression analysis
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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