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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
p-mentha-1,4-diene
EC Number:
202-794-6
EC Name:
p-mentha-1,4-diene
Cas Number:
99-85-4
Molecular formula:
C10H16
IUPAC Name:
p-mentha-1,4-diene
Specific details on test material used for the study:
Liquid, Stable at room temperature

Sampling and analysis

Analytical monitoring:
yes
Remarks:
chromatographic separation (HPLC) with UV/DAD detection (ultraviolet/diode array detector).

Test organisms

Test organisms (species):
activated sludge, domestic

Study design

Test type:
not specified
Water media type:
other: Tap water
Total exposure duration:
3 h

Test conditions

Test temperature:
20 ± 2ºC
pH:
7.30
Nominal and measured concentrations:
One replicate for concentration (10 mg L-1)
One replicate for concentration (100 mg L-1)
Three replicates for concentration (1000 mg L-1)
Reference substance (positive control):
yes
Remarks:
copper sulfate pent hydrate

Results and discussion

Effect concentrations
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance was solubilized in water because of its solubility. As there were no statistically significant toxic effects at the highest concentration (1000 mg L-1), no further testing was required at higher or lower concentrations as outlined in OECD 209, 2010.