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Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997-09-26 till 1997-10-22
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: Official Journal of the EC L 133 Part C: Biodegradability: Examination of the respiratory inhibition
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Principles of method if other than guideline:
A defined amount of activated sludge is mixed with synthetic medium and measured the rate of respiration. This respiration rate is compared with those in the test preparations with different concentrations of the test substance be measured. The sensitivity of the activated sludge is examined with 3.5-dichlorophenol as a reference.
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Type and Origin: Activated sludge (mixed population of various micro-organisms) from a laboratory facility with the municipal waste water is mainly operated (southern Wupperverband).
Proparation of microbial inoculum:
- not pretreatment
- pH of the culture solution before Depletion: 7.6
- adjusted actived sludge was 2 g/L (dry weight)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20 ± 2 °C
pH:
7.1 - 7.7
Dissolved oxygen:
Experiments with test substance: 3.4 - 8.1 mg/L
Experiments with reference substance: 3.8 - 9.3 mg/L
Control experiments: 3.0 - 6.5 mg/L
Physico-chemical oxygen consumption control: 9.8 mg/L
Nominal and measured concentrations:
- Nominal concentration of test substance: 100, 180, 320, 560 and 1000 mg/L
- Nominal concentration of reference concentration: 2.5, 5, 10, 20 and 40 mg/L
Details on test conditions:
- Two control whitout test substance at test start and test end were performed in presence of activated sludge
- One physico-chemical oxygen consumption was carried out at the hightest test concentration of 1000 mg/L in absence of activated sludge.

A synthetic medium was made by dissolving the following amounts of substance in 1 litre of water.
64 g peptone
44 g meat extract
12 g urea
2.8 g NaCl
1.6 g CaCl2 x 2H2O
0.8 g MgSO4 x 7 H2O
11.2 g K2PO4
Reference substance (positive control):
yes
Remarks:
3.5-Dichlorophenol (purity: 97%)
Duration:
3 h
Dose descriptor:
other: EC05
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 6.2 - 72
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
62 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 17 - 111
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
473 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 340 - 758
Duration:
3 h
Dose descriptor:
other: EC90
Effect conc.:
3 620 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 1700 - 21400
Duration:
3 h
Dose descriptor:
other: EC95
Effect conc.:
6 440 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 2580 - 57600

Test substance: 1,2,5,6 -Tetrahydrobenzaldehyd

 Test concentration[mg/L]  Respiration rate[mg/L*h]  Inhibition[%]
 100  26.0  16.7
 180  22.8  26.9
 320  18.9  39.4
 560  14.0  55.1
 1000  10.0  67.9
Validity criteria fulfilled:
yes
Remarks:
respiratory rates of the 2 controls differ less than 15 % from each other. EC 50 of the reference compond 3.5-Dichlorophenol was in the range 5 - 20 mg/L.
Conclusions:
The respiration inhibition on activated sludge whitin 3 hours was measured at EC 50 of 473 mg/L.
Executive summary:

The potenial toxic effect of cyclohex-3-ene-1-carbaldehyde was tested according to official journal of the EC L 133 Part C, which is equal to OECD Guideline 209. The respiration inhibition on activated sludge whitin 3 hours was measured at EC 50 of 473 mg/L.

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
August 1979 til Februar 1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable with restrictions.
Qualifier:
according to guideline
Guideline:
other: Zellvermehrungstest nach Bringmann & Kühn (1977, page 87-98)
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
pre-dissolved with ethanol
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Nominal and measured concentrations:
Nominal concentration: 1.95 mg/L - 1000 mg/L
Duration:
16 h
Dose descriptor:
other: EC3
Effect conc.:
66 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Cell proliferation inhibition

Exposure period: 16 - 18 hours

Description of key information

The respiration inhibition on activated sludge whitin 3 hours was measured at EC 50 of 473 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
473 mg/L

Additional information