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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Isopropenyl acetate was assessed for its ready biodegradability  in a Closed Bottle Test according to OECD 301 D. The test item is considered to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Isopropenyl acetate was assessed for its ready biodegradability in a Closed Bottle Test according to OECD 301 D. Activated sludge (micro organisms from a domestic waste water treatment plant) was supplied by the sewage plant Darmstadt, Germany. The test item Isopropenyl acetate was investigated for its ready biodegradability in a Closed Bottle Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. A reference item was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The test concentration was 5 mg/L corresponding to an oxygen demand of 9.59 mg/L based on a ThOD of 1.918 mg oxygen per mg test item. Under the test conditions the percentage biodegradation of Isopropenyl acetate reached 77% (mean value) after 28 days of incubation.

The test item is considered to be readily biodegradable.The reference item sodium benzoate was sufficiently degraded to a mean of 76% after 14 days, and to a mean of 75 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item sodium benzoate, a mean of 41 % biodegradation was noted within 14 days and 38 % biodegradation after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge microorganisms.