Bis(1-methylheptyl) adipate

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Assessment of the safety of dioctyl adipate in freshwater environments

Author:

Felder, et al.

Year:

1986

Bibliographic source:

Environ Toxicol Chem 5:777-784

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ASTM E-35.32 (1979) (Proposed Draft)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2-ethylhexyl) adipate

EC Number:

203-090-1

EC Name:

Bis(2-ethylhexyl) adipate

Cas Number:

103-23-1

Molecular formula:

C22H42O4

IUPAC Name:

bis(2-ethylhexyl) adipate

Test material form:

liquid

Specific details on test material used for the study:

The test substance is listed as Dioctyl adipate or di(2-ethylhexyl) adipate.

Radiolabelling:

yes

Sampling and analysis

Details on sampling:

The test concentration was confirmed by radioanalysis before the fish were introduced.

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

The test organism is listed as the bluegill sunfish (Lepomis macrochirus).

Study design

Route of exposure:

aqueous

Test type:

not specified

Water / sediment media type:

natural water

Remarks:

well water

Total exposure / uptake duration:

28 d

Total depuration duration:

14 d

Test conditions

Hardness:

Hardness of 265 ± 10 mg/L,

pH:

7.8 to 8.3

Dissolved oxygen:

9.2 to 10.2 mg/L dissolved oxygen concentration

Conductivity:

700 p.0 /cm.

Nominal and measured concentrations:

The bioconcentration study evaluated the effects of the test substance with a concentration of 0.20 mg/L. The test concentration was confirmed by radioanalysis before the fish were introduced.

Reference substance (positive control):

not specified

Results and discussion

Bioaccumulation factor

Key result

Conc. / dose:

ca. 0.2 mg/L

pH:

8.05

Type:

BCF

Value:

27 dimensionless

Basis:

other: Based on 14C determinations and not on measurement of actual test substance concentrations in fish tissue

Remarks:

.

Time of plateau:

1 d

Depuration

Elimination:

yes

Parameter:

DT50

Depuration time (DT):

1 d

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The whole-fish BCF for the test substance was determined to be 27 for bluegills exposed to the concentration tested (nominal concentration of 0.20 mg/L) and 250 ± 80 ug/L [14C]DOA at test termination on day 28. This relatively low BCF indicates that fish exposed to concentrations of the substance in water or food chain will not be adversely affected.