Tridecan-1-ol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

Aim of this study was to determine the toxic nature of test chemical on the growth of microorganism Tetrahymena pyriformis.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Tetrahymena pyriformis

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Details on test conditions:

Details on test conditions
TEST SYSTEM
- No. of vessels per concentration (replicates): 3
- Other: Each replicate was a five-step graded concentration series using freshly prepared stock solutions. Only replicate with control cultures in the late log-growth-phase (absorbance of 0.6 to 0.9) were used in these studies.

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

538.96 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: IGC50 present as log inverse IGC50 (2.28 mM)

Reported statistics and error estimates:

Probit Analysis of SAS [Statistical Analysis System] with Y being the absorbance normalized to percent control and X being the concentration of the toxicant.

Validity criteria fulfilled:

not specified

Conclusions:

Based on the growth rate inhibition of test organisms Tetrahymena pyriformis due to the exposure of test chemical the IGC50 was 586 mg/l.

Executive summary:

Aim of this study was to determine the toxic nature of test chemical on the growth of microorganism Tetrahymena pyriformis for the total exposure period of 48 hrs. Chemical purchased from Aldrich Chemical Co., Milwaukee, Wisconsin having 95 % purity. Test performed in 3 replicates and each replicate was a five-step graded concentration series using freshly prepared stock solutions. Only replicate with control cultures in the late log-growth-phase (absorbance of 0.6 to 0.9) were used in these studies. Based on the growth rate inhibition of test organisms Tetrahymena pyriformis due to the exposure of test chemical for 48 hrs, the IGC50 was determine to be 586 mg/l.

Description of key information

Aim of this study was to determine the toxic nature of test chemical on the growth of microorganism Tetrahymena pyriformis for the total exposure period of 48 hrs. Chemical purchased from Aldrich Chemical Co., Milwaukee, Wisconsin having 95 % purity. Test performed in 3 replicates and each replicate was a five-step graded concentration series using freshly prepared stock solutions. Only replicate with control cultures in the late log-growth-phase (absorbance of 0.6 to 0.9) were used in these studies. Based on the growth rate inhibition of test organisms Tetrahymena pyriformis due to the exposure of test chemical for 48 hrs, the IGC50 was determine to be 586 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

586 mg/L

Additional information

Various experimental data for the target chemical have been reviewed to determine the toxic nature of test substance on the growth of microorganisms. The studies are as mentioned below:

In the first key study for the target chemical from peer reviewed journal , Aim of this study was to determine the toxic nature of test chemical on the growth of microorganism Tetrahymena pyriformis for the total exposure period of 48 hrs. Chemical purchased from Aldrich Chemical Co., Milwaukee, Wisconsin having 95 % purity. Test performed in 3 replicates and each replicate was a five-step graded concentration series using freshly prepared stock solutions. Only replicate with control cultures in the late log-growth-phase (absorbance of 0.6 to 0.9) were used in these studies. Based on the growth rate inhibition of test organisms Tetrahymena pyriformis due to the exposure of test chemical for 48 hrs, the IGC50 was determine to be 586 mg/l.

First experimental study was supported by the second supporting study from peer reviewed journal. In a 48 hours short team toxicity study, the effect of test material was evaluated on 484 Tetrahymena pyriformis Ciliate. The test substance was test on the basis of QSAR in a concentration of 1001.5 mg/L. The results show 50% inhibition in growth rate of test organism’s population at given concentration. Therefore, IC50 for test material was considered to be growth inhibiter at 1001.5 mg/L when tested on Tetrahymena pyriformis Ciliate for 48hrs.

 

Similarly in the third supporting study test chemical from another peer reviewed journal study was conducted. In a 48 hours short team toxicity study, the effect of test material was evaluated on 484 Tetrahymena pyriformis Ciliate. The test substance was test on the basis of QSAR, TOXICITY and BCF MODELS in a concentration of 1.05 mg/L (1050 µg/L). The results show 50% inhibition in growth rate of test organism’s population at given concentration. Therefore, IC50 for test material was considered to be growth inhibitor at 1.05 mg/L (1050 µg/L) when tested on 484 Tetrahymena pyriformis Ciliate for 2 days.

 

The first two study supports the nontoxic nature of chemical but according to the remaining study chemical was toxic and the MIC ranges from 1.05 mg/l to 1001.5 mg/l.