Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-524-2 | CAS number: 122-14-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 March 1987 - 09 March 1987
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-1 (Fish Acute Toxicity Test)
- Version / remarks:
- U.S. EPA FIFRA, 40 CFR, Part 158.145 Guideline 72-1 (1982)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fenitrothion
- EC Number:
- 204-524-2
- EC Name:
- Fenitrothion
- Cas Number:
- 122-14-5
- Molecular formula:
- C9H12NO5PS
- IUPAC Name:
- O,O-dimethyl O-3-methyl-4-nitrophenyl phosphorothioate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 60553
- Purity (measured): 94.5%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature Darkness
- Solubility and stability of the test substance in the solvent/vehicle: Stable during the 96 hrs test
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.6, 1.2, 2.5, 5.0 and 10 mg/L (nominal conc.)
- Sampling method: 0hr and 96 hrs
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Stock solution:13.03g of Test substance dissolved in 100 mL of DMF aliqutted to different test media at different ration to achieve 0.6, 1.2, 2.5, 5.0 and 10 mg/L (nominal conc.). The measured concentrations yielded an average of 36 ± 14% of nominal at the 0-hour sampling (excluding the lowest exposure level affected by the diluter malfunction). The measured concentrations yielded an average of 39 ± 23% of nominal at the 96-hour sampling (excluding the lowest exposure level affected by the diluter malfunction).
Test organisms
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
-Common name: Bluegill Sunfish, Source: Osage Catfisheries, Inc. Osage Beach, Missouri Weight at study initiation (mean and range, SD): 3.8 g(+-0.98)
ACCLIMATION
-Acclimation conditions (same as test or not): 16:8 light dark cycle, 18 °C. Type and amount of food during acclimation: Brine shrimp daily. Feeding frequency during acclimation: daily. Acclimation period: 48 hrs starvation prior to test initiation,
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 225-275 CaCO3 equivalent ppb
- Test temperature:
- 21°C
- pH:
- 7.9-8.1
- Dissolved oxygen:
- 6.8-8.1 mg/L (76%ASV- 90%ASV)
- Nominal and measured concentrations:
- Nominal concentration: 0.6, 1.2, 2.5, 5.0 and 10 mg/L Measured concentrations: 0.38, 0.69. 1.0, 1.2 and 6.4 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 30L
- Type (delete if not applicable): covered with lid
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter, intermittent dosing 9x1L/hr
- Renewal rate of test solution (frequency/flow rate): 7xreplacement using a diluter
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: 0.36/L/24hrs
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Nominally 50% (this was not met due to diluter failiure and TS insolubility)
TEST SYSTEM
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter, intermittent dosing 9x1L/hr
- Renewal rate of test solution (frequency/flow rate): intermittent dosing 9x1L/hr 7 replacement per day
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.36/L/24hrs
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water
- Total organic carbon: 1.6ppm
- Alkalinity: 325-375ppm CaCO3
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Nominally 50% (this was not met due to diluter failiure and TS insolubility)
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.69 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Details on results:
- Behavioural/sublethal effects noted at 6.4, 1.2, and 1.0mg/L during the study included loss of equilibrium and on bottom orientation. One fish died in each of the control and solvent control aquaria. This percentage of mortality in the controls did not comprise the toxicity results according to the criteria and was likely caused from handling stress during the assigment of fish to the test container. Given that mortality and/or behavioural/sublethal effects occurred at 6.4, 1.2, and 1.0mg/l, a no-effect concentration of fenitrothion toxicity to bluegill sunfish was determined to be 0.69 mg/l.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortality and behavioural observations
Mean measured concentrations
(Nominal concentrations) (mg/L)
No. of fish
24 hours
48 hours
72 hours
96 hours
Cum.
Mort.
Obs.
Cum.
Mort.
Obs.
Cum.
Mort.
Obs.
Cum.
Mort.
Obs.
Control
20
0
19N
1LOE/OB
1
19N
1
19N
1
19N
Solvent control
20
0
20N
1
19N
1
19N
1
19N
1.0 (0.60)
20
0
20N
0
19N
1LOE/OB
1
19N
1
19N
0.38 (1.2)
20
0
20N
0
20N
0
20N
0
20N
0.69 (2.5)
20
0
20N
0
20N
0
20N
0
20N
1.2 (5.0)
20
1
19N
1
18N
1OB
1
18N
1OB
1
19N
6.4 (10)
20
17
3LOE
20
---
20
---
20
---
Cum. Mort.= Cumulative Mortality; Obs.= Observations
N= Normal; LOE= Loss of Equilibrium; OB= On Bottom Orientation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the mortality obtained from the Fenitrothion exposure range: 0.38, 0.69. 1.0, 1.2 and 6.4 mg/L (measured) (equivalent to 0.6, 1.2, 2.5, 5.0 and 10 mg/L (nominal conc.) the 96 hr LC50 value and 95% confidence interval was 2.5 mg/L (2.0 -3.2 mg/L) based on the average measured concentrations at 0 and 96 hrs. Behavior/sublethal effects occured at 6.4, 1.2 and 1.0 mg/L, a NOEC of Fenitrothion toxicity to bluegill sunfish was determined to be 0.69 mg/L. This conclusion was supported by the lack of mortality or behavioral/sublethal effects at and below the 0.69 mg/L test level.
- Executive summary:
A 96 hour acute flow through toxicity study of Fenitrothion to bluegill sunfish (Lepomis macrochirus) was conducted. Twenty fish were used in each of five test concentrations plus Solvent Control and Well-water Control. The nominal dosing regime was 0.6, 1.2, 2.5, 5.0 and 10 mg/L based on 94.6% purity of Fenitrothion. Analytical measurements were made at 0 and 96 hrs. The measured concentrations were 0.38, 0.69. 1.0, 1.2 and 6.4 mg/L. The 96 hr LC50 value and 95% confidence interval was 2.5 mg/L (2.0 -3.2 mg/L) based on the average measured concentrations at 0 and 96 hrs. NOEC of Fenitrothion toxicity to bluegill sunfish was determined to be 0.69 mg/L. This conclusion was supported by the lack of mortality or behavioural/sublethal effects at and below the 0.69 mg/L and 0.38 mg/L test levels.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.