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Diss Factsheets
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EC number: 206-462-1 | CAS number: 345-78-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study in compliance with GLP-criteria.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pseudoephedrine hydrochloride
- EC Number:
- 206-462-1
- EC Name:
- Pseudoephedrine hydrochloride
- Cas Number:
- 345-78-8
- Molecular formula:
- C10H15NO.ClH
- IUPAC Name:
- 2-(methylamino)-1-phenylpropan-1-ol hydrochloride
- Details on test material:
- - Name of test material (as cited in study report): (+)-Pseudoephedrin-Hydrochlorid
- Aggregate state/appearance: solid (crystalline0 / white
- Homogeneity: homogeneous
- Storage conditions: ambient (RT), light exclusion
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start (after 0h) and the end (after 72h) of the test the uninoculated replicates were analyzed.
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
The test substance was stirred in deionized water for about 20 min at approximately 20 ± 2 °C. The stock solution was clear and colourless. The nominal concentration of the stock solution was 125.3 mg/L. Following this, serial dilutions of this stock solution were prepared in order to obtain the desired test concentrations.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The test strain of Desmodesmus subspicatus CHODAT SAG 86.81 is obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and is kept in liquid culture in the Laboratory of Experimental Toxicology and Ecology.
PRECULTURE TECHNIQUES:
- Seed-culture: a seed culture was incubated for 4 days at 23 ± 2°C; final cell density: 1.92E+06 cells/mL
- Pre-cutture: seed-culture was taken to inoculate a pre-culture (initial cell density: 1E+04 cells/mL) . The pre-culture was incubated for 3 days at 23 ± 2°C. Final cell density : 4.4E+05 cells/mL.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 ± 2 °C
- pH:
- 7.9 - 8.2
- Nominal and measured concentrations:
- - Nominal test concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L (based on range finding test)
- Measured concentrations: The nominal concentrations of 0 and 100 mg/L were analysed at t=0 and at t= 72 h.
- t=0: n.d. and 99.0 mg/L
- t=72h: n.d. and 98.7 mg/L - Details on test conditions:
- TEST SYSTEM:
- Test vessel: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable siliconsponge caps
- Fill volume: 100 mL
- Initial cells density: 1E+04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS:
- Source/preparation of dilution water: according to OECD guidelines
OTHER TEST CONDITIONS:
- Photoperiod: permanent illumination
- Illumination: artificial light, type universal white (OSRAM L 25)
- Light intensity: about 60 - 120µE/(m2·s) at a wave length of 400 - 700 nm
EFFECT PARAMETERS MEASURED:
- Measurement of fluorescence: after 0, 24, 48 and 72 h
- Determination of cell concentrations: counting chamber (Neubauer improved)
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) - Reference substance (positive control):
- yes
- Remarks:
- Potassiumdichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 29.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 13.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 48.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- The72-h EbC50 (72 h) was 0.48 mg/L (date of the last control experiment: 25 Nov 2002).
- Reported statistics and error estimates:
- The EC values are calculated (linear regression analysis) from the concentration-response relationship. The Dunnett's test (one-sided) is carried out at a 95 % significance level.
Any other information on results incl. tables
Inhibition of growth rate:
Nominal conc. (mg/L) |
Growth inhibition (%) |
0 (control) |
0.0 |
12.5 |
2.9 |
25 |
7.2 |
50 |
19.6 |
100 |
41.6 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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