Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-351-3 | CAS number: 613-73-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- o-phenylenediacetonitrile
- EC Number:
- 210-351-3
- EC Name:
- o-phenylenediacetonitrile
- Cas Number:
- 613-73-0
- Molecular formula:
- C10H8N2
- IUPAC Name:
- 2-[2-(cyanomethyl)phenyl]acetonitrile
- Test material form:
- solid: particulate/powder
Constituent 1
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 16 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 127 mg/L
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1 140 mg/L
Applicant's summary and conclusion
- Executive summary:
The inhibitory effect of l,2-Phenylendiacetonitrile on bacteria multiplication was tested by the
Pseudomonas cell multþlication inhibition test according DIN 38 412, part 8.
The bacteria \ilere exposed to the substance in a concentration range from 75 mgll to 2000 mg/l.
After 16 hours incubation the cell multiplication was determined by measuring the degree of
turbidity. The top concentration of 2000 mgl caused an inhibitory effect of 80 % ,The calculated inhibition
values of each test concentration were evaluated by regression analysis. The concentrations of l0 %
and 50 % reduction in multiplication relative to the control were calculated by regression analysis.
The values of the effective concentrations were derived from the regression function:
EC10: 127 mg/l
EC50 1140 mg/l
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
