1,3,2-dioxathiane 2,2-dioxide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD 202 (2004)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material: 1,3,2-dioxathiane 2,2-dioxide
- IUPAC name: 1,3,2-Dioxathiane 2,2-dioxide
- Molecular formula: C3H6O4S
- Molecular weight: 138.14 g/mol
- Substance type: Organic
- Physical state: Solid

Vehicle:

not specified

Details on test solutions:

The primary stock solution used in the range-finding test was prepared by dissolving 10 mg of the test substance in 40 mL of dilution water – M4 and the total volume was completed to 100 mL, resulting in a concentration of 100 mg.L-1. To assist solubilization, the solution was stirred for 20 minutes in a magnetic stirrer and 5 minutes of ultrasonic dispersion was also applied. The secondary stock solutions of the 10 and 1 mg.L-1 were prepared by dilutions of the primary stock solution.

The stock solution used in the definitive test was prepared by dissolving 50 mg of the test substance in 40 mL of dilution water – M4 and the total volume was completed to 500 mL, resulting in a concentration of 100 mg.L-1. To assist solubilization, the solution was stirred for 20 minutes in a magnetic stirrer and 5 minutes of ultrasonic dispersion was also applied.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test system used for this study is described below, according to Straus (1820):

Kingdom: Animalia
Phylum: Arthropoda
Subphylum: Crustacea
Class: Branchiopoda
Order: Cladocera
Family: Daphniidae
Genus: Daphnia
Species: Daphnia magna

Young Daphnia magna ≤ 24 hours old were obtained from the culture kept at the Ecotoxicology Laboratory, maintenance and cultivation records are archived at BIOAGRI.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

185.2 mg.L-1 CaCO3

Test temperature:

20.0 to 20.1 °C

pH:

7.7

Dissolved oxygen:

8.0 to 8.1 mg.L-1

Nominal and measured concentrations:

In the range-finding test the exposure conditions were: control; 0.1; 1; 10 and 100 mg.L-1
In the definitive test the exposure conditions were: control; 3.3; 6.5; 13; 25 and 50 mg.L-1

Details on test conditions:

TEST SYSTEM
Test vessel: 30 mL glass beakers

In the range-finding test the exposure conditions were:

a) Total number of organisms per each tested concentration or control: 10;
b) Number of replicates per each tested concentration and control: 2;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 0.1; 1; 10 and 100 mg.L-1;
g) Temperature of the test solutions: 20.1 to 20.3°C;
h) Photoperiod: 16 hours of light and 8 hours of dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks.

In the definitive test the exposure conditions were:

a) Total number of organisms per each tested concentration or control: 20;
b) Number of replicates per each tested concentration and control: 4;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 3.3; 6.5; 13; 25 and 50 mg.L-1;
g) Temperature of the test solutions: 20.0 to 20.5°C;
h) Photoperiod: 16 hours of light and 8 hours of dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks;
j) Analytical determination: content dissolved oxygen, pH of the test solutions and the test substance concentration were determined at the beginning of the test and after 48 hours.


TEST MEDIUM / WATER PARAMETERS
Source/preparation of dilution water: M4 (OECD 202)
pH: 7.7
Hardness: 185.2 mg.L-1 CaCO3
Dissolved oxygen: 8.0 to 8.1 mg.L-1
Temperature: 20.0 to 20.1°C

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 18.01 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

95% confidence limits = 14.53 to 22.32 mg.L-1

Basis for effect:

not specified

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 38.56 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

95% confidence limits = 29.64 to 50.16

Basis for effect:

not specified

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 3.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

other: immobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 50 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

other: immobilization

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50 (24 h): 1.03 mg/L (95% confidence limits = 0.91 to 1.17 mg.L-1).

Validity criteria fulfilled:

yes

Conclusions:

The 48-hours EC50 value of 1,3,2-Dioxathiane, 2,2-dioxide was 18.01 mg.L-1 (95% confidence limits = 14.53 to 22.32 mg.L-1).

Executive summary:

Assessing the toxicity to aquatic invertebrates, Daphnia magna was exposed to 1,3,2-dioxathiane 2,2-dioxide for 48 hours. The experiment was conducted according to OECD Guideline 202 (2004). The test gave a 48-h EC50 value of 18.01 mg.L-1 (95% confidence limits = 14.53 to 22.32 mg.L-1).

Description of key information

Study carried out according to guideline and with GLP compliance.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

18.01 mg/L

Additional information

In a static test conducted according to OECD 202 guideline an EC50 of 18.01 mg/L after 48 h based on mean measured concentration has been determined.