Trisodium [29H,31H-phthalocyaninetrisulphonato(5-)-N29,N30,N31,N32]cuprate(3-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980-1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

Methodology comparable to internationally accepted guidelines

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Test organisms (species):

other: Oncorhynchus mykiss and Danio rerio

Details on test organisms:

Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Zebrafish: (Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Rainbow trout: 15 °C
Zebra fish: 23 °C

pH:

8 - 8.2 (Rainbow trout)
8.1 (Zebra fish)

Dissolved oxygen:

7.8 mg/L (Rainbow trout)
7 - 7.4 mg/L (Zebra fish)

Nominal and measured concentrations:

100, 300 and 1000 mg/L (Rainbow trout)
300 and 1000 mg/L (Zebra fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: for Rainbow trout

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: for Zebra fish

Sublethal observations / clinical signs:

No mortality observed at any of the tested concentrations.

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (described as TL50) for Rainbow trout as well as Zebra fish was determined to be >1000 mg/L.

Executive summary:

The acute fish toxicity of FAT 20063/B was assessed in a study conducted using the methodology equivalent to OECD Guideline 203. Three groups consisting of 8 (Rainbow trout) fish each, were exposed to 100, 300 and 1000 mg/ L test concentrations for 96 hours, while two groups of 8 (Zebra fish) each were exposed to 300 and 1000 mg/L test concentrations for 96 hours. No mortality was observed at any of the tested concentrations. Hence, the LC50 (described as TL50) for Rainbow trout as well as Zebra fish was determined to be >1000 mg/L.

Description of key information

LC50 for fish is >1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

Short term toxicity studies of the substance with fish are carried out with rainbow trout and zebra fish. both the studies indicated the LC50 to be >1000 mg/L.