Registration Dossier
Registration Dossier
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EC number: 222-321-7 | CAS number: 3425-61-4
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
There is no test performed with Tert-amyl hydroperoxide. The acute toxic effects on aquatic invertebrates of this substance are read-across from data obtained with1,1,3,3-Tetramethylbutyl hydroperoxide: an acute toxicity test to Daphnia magna was conducted in accordance with OECD test guidelines and with the OECD Principles of Good Laboratory Practice: EC50-48h = 6.7 m/L (Measured Mean).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 6.7 mg/L
Additional information
- Immobilization in the control did not exceed 10%
- Oxygen concentration did not fall below 3 mg/L at any point during the study
- The EC50 value of the reference compound, potassium dichromate, was in the range of 0.25-2.0 mg/L (Documented as part of GLP laboratory maintenance).
- No daphnids were trapped on the surface for any length of time.
- The analytical validity criteria set in the study plan were met.
There is no test performed with Tert-amyl hydroperoxide. The toxic effects on aquatic invertebrates of this substance is read-across from data obtained with1,1,3,3-Tetramethylbutyl hydroperoxide.
In order to predict the effects of 1,1,3,3-Tetramethylbutyl Hydroperoxide in an aquatic environment, an acute toxicity test to Daphnia magna was conducted in accordance with OECD test guidelines and with the OECD Principles of Good Laboratory Practice.
The toxicity of the test chemical and any formed degradation products to juvenile daphnia was determined in a static system over an exposure period of 48 hours. The following concentrations were tested 0.6, 1.4, 3.0, 6.8 and 15 mg/L. The NOEC was determined as 4.21 mg/L (Measured Mean Concentration). The EC50 (48h) was calculated as 6.7 mg/l. (Measured Mean).
Chemical analysis was conducted at the start and at the end of the study. Analytical quality criteria were met. Geometric measured mean concentrations were calculated for use in the end point calculations.
The following quality criteria were met in the final definitive study:
The following quality criterion was not met:
The test substance did not remain stable during the test.
Investigation of the effects of the non readily biodegradable degradation products would benefit further risk assessment of this substance
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