(2,2-dimethyl-1,3-dioxolan-4-yl)methyl methacrylate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: Pseudokirchneriella subcapitata HINDAK, SAG 61.81
- Source (laboratory, culture collection): Collection of algal cultures in University of Göttingen/Germany

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Details on test conditions:

TEST SYSTEM
- Test vessel:Sterile Erlenmeyer flasks (volume 250 ml; sealed with cotton wool plugs)
- Type (delete if not applicable): closed
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH:no
- Photoperiod: 24 hours/day light
- Light intensity and quality: Approximately 4440 to 8880 lux, corresponding to 60 to 120 µ E*m-2*s-1

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

402 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

116 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

15.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The study was conducted under static conditions with an initial cell density of 6305 cells/ml. A stock solution of 500 mg/L was freshly prepared with demineralized water and stirred for 15 minutes (1100 rpm, room temperature). Seven concentrations were tested in a geometrical series with a dilution factor of 3.2: 0.466; 1.49; 4.77; 15.3; 48.8; 156.3; 500 mg/L, corresponding to the geometric mean measured test item concentrations: 0.402; 1.43; 4.90; 15.4; 53.3;
178; 558 mg/L. The environmental conditions were within the acceptable limits. The concentrations of the test item in all test concentrations and the control were analytically verified by GC-MS at the start and the end of exposure. The measured concentrations of IPGMA in the fresh media (0 h) were in the range of 104 to 120% of the nominal values. The measured concentrations in the old media (72 h) were in the range of 72 to 108% of the nominal values.

	Inhibition of Growth Rate
NOEC	15.4
LOEC	53.3
ErC10	116 (89.9 – 135)
ErC20	202 (185 – 324)
ErC50	402 (344 – 455)
	Inhibition of Yield
NOEC	1.43
LOEC	4.90
EyC10	n.d.
EyC20	34.8 (20.8 – 55.7)
EyC50	125 (104 – 148)

Validity criteria fulfilled:

yes

Conclusions:

In this study, Isoporopylidenglycerolmethacrylate (IPGMA) was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values: Based on the geometric mean measured test item concentrations, the NOEC values (0-72 hours) for inhibition of growth rate and inhibition of yield were 15.4 mg/L and 1.43 mg/L. The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 402 (344 - 455) mg/L and 125 (104 - 148) mg/L, respectively.

Description of key information

In this study, Isopropylidenglycerolmethacrylate (IPGMA) was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values:

Based on the geometric mean measured test item concentrations, the NOEC values (0-72 hours) for inhibition of growth rate and inhibition of yield were 15.4 mg/L and 1.43 mg/L. The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 402 (344 - 455) mg/L and 125 (104 - 148) mg/L, respectively.

Key value for chemical safety assessment

EC50 for freshwater algae:

402 mg/L

EC10 or NOEC for freshwater algae:

15.4 mg/L