Dipotassium tin hexahydroxide

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

read accross to Potassium Hexahydroxostannate, based onb the effects of sodium and potasium cation is not relevant

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Hardness:

0.24 mmol Ca+Mg/L.

Test temperature:

Nominal range: 21 - 24 °C, controlled at ± 2 °C

pH:

pH - neutralized 8.24
ph (100 mg/L) 9.21

Dissolved oxygen:

see study report

Salinity:

see study report

Conductivity:

see study report

Nominal and measured concentrations:

see study report

Details on test conditions:

see study report

Reference substance (positive control):

yes

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.318 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

37.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

see study report

Results with reference substance (positive control):

see study report

Reported statistics and error estimates:

see study report

Validity criteria fulfilled:

yes

Conclusions:

The RC50 for growth inhibition rate of algae for Potassium Hexahydroxostannate qA determined to be 37.9 mg/l

Executive summary:

The toxicity of Potassium Hexahydroxostannate, crystalline M (batch number: 25L138) to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 at Dr.U.Noack-Laboratorien in 31157 Sarstedt, Germany from 2013-05-13 to 2013-05-16. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.

The study was conducted under static conditions with an initial cell density of 4332 cells/mL. Based on a preliminary test 7 concentration levels were tested in a geometrical series with a dilution factor of 3.16 (nominal): 0.100 - 0.316 - 1.00 - 3.16 - 10.0 - 31.6 - 100 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. Three replicates of the highest test concentration were additionally tested with an initial pH value of 8.24 to test for pH-related effects. Environmental conditions were determined to be within the acceptable limits.

The concentrations of Potassium Hexahydroxostannate, crystalline M in all test concentrations, the pH - Control and control were analytically verified via ICP-OES by an external test facility (non-GLP, see page 4) after 0 and 72 hours. The measured concentrations at start of the exposure were in the range of 100 to 108 % of the nominal values. Recovery rates at the end of the exposure were in the range of 91 % to 102 % of the nominal values

Key value for chemical safety assessment

EC50 for freshwater algae:

37.9 mg/L

EC10 or NOEC for freshwater algae:

0.318 mg/L