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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 days
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Justification for type of information:
Peer reviewed publication describing research work in good detail

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2005

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Version / remarks:
14 days exposure
Principles of method if other than guideline:
Different soil types were assessed as well as effect of pH
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Chromic chloride
IUPAC Name:
Chromic chloride

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
6-week-old worms of uniform size (300–400 mg) were used

Study design

Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
14 d
Post exposure observation period:
No

Test conditions

Test temperature:
28 C
pH:
5.72 - 7.12 depending on soil types
Moisture:
moisture content 40% to 60%
Details on test conditions:
The soils were air-dried and sieved through a 1-mm sieve for use in the analysis of various soil properties.
Dried, urine-free cow dung manure was ground and sieved (particle size, 500 to p100 mm) to serve as the organic substrate medium.
Nominal and measured concentrations:
500, 1000, 1500, 2000, 2500, and 3000 mg/kg as Chromium III

Results and discussion

Effect concentrations
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 635 - < 1 902 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Basis for effect:
mortality
Details on results:
Slight differences were seen between the different soil types, but this is not significant.

Any other information on results incl. tables

Changes were not observed in the morphology of earthworms or their burrowing behaviour in both soil and organic media at all concentrations

Applicant's summary and conclusion

Conclusions:
Toxicity was observed at high concentrations, but surviving worms showed no morphological changes.
The same study looked at toxicity of Chromium VI (potassium dichromate) and this demonstrated much higher toxicity.