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EC number: 244-599-9 | CAS number: 21829-50-5
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48-h EC50 value for monoethanolamine in Daphnia magna is 65 mg/L, the 24-h LC50 value for citric acid in Daphnia magna is 1,535 mg/L. After correction for molecular weight the effect concentrations for tris[(2-hydroxyethyl)ammonium] citrate are 399 and 2,999 mg/L, respectively. The lowest value of 399 mg/L will be used in the assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 399 mg/L
Additional information
Short-term studies with invertebrates are not available for tris[(2-hydroxyethyl)ammonium] citrate. Tris[(2-hydroxyethyl)ammonium] citrate is the neutral salt of monoethanolamine and citric acid. Monoethanolamine and citric acid are the starting materials for the synthesis of tris[(2-hydroxyethyl)ammonium] citrate, and the only difference is that in tris[(2-hydroxyethyl)ammonium] citrate the substances are present in their ionic forms as a monoethanolamine cation and a citrate anion. It is expected that the ecotoxicological properties of tris[(2-hydroxyethyl)ammonium] citrate shall be governed by the properties of monoethanolamine and citric acid (for more details see reporting format for the analogue approach in Appendix A.2).
A GLP-compliant Directive 92/69/EEC, C.2. guideline study with Daphnia magna is available for monoethanolamine (Huels, 1997b). Daphnids (20/dose) were exposed to nominal concentrations of 3 to 1,000 mg/L under semi-static conditions for 48 hours, the test concentrations were analytically verified and were within 80% of nominal concentrations. The 48-h EC50 value is 65 mg/L (based on nominal concentrations), a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is 399 mg/L.
For citric acid a non-GLP study equivalent to the OECD guideline 202 study is available (Bringmann and Kühn, 1982) with Daphnia magna. Daphnids (20/dose) were exposed to citric acid (doses not know) under static conditions for 24 hours; the test concentrations were not analytically verified. The 24-h EC50 value is 1,535 mg/L, a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is 2,999 mg/L.
The lowest effect concentration of 399 mg/L will be used in the assessment.
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