2-[(1-amino-9,10-dihydro-4-hydroxy-9,10-dioxo-2-anthryl)oxy]ethyl phenyl carbonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

In principle the methods described in OECD Guideline 203 were used.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

FAT 36052/D was dissolved with acetone and in parallel and in another experiment FAT 36052/D was dissolved in Tween 80.

Test organisms (species):

other: Trout

Details on test organisms:

8 Trouts for each experiment.

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Test temperature:

14 °C

pH:

8-8.1

Nominal and measured concentrations:

Nominal concentration used for FAT 36052/D dissolved with acetone and dissolved with Tween 80:
2 mg/l
20 mg/l
100 mg/l (Sediment)
200 mg/l
1000 mg/l

Details on test conditions:

8 trouts were used for the experiment where FAT 36052/D was dissolved with acetone and 8 trouts were used in the experiment where FAT 36052/D was dissolved with Tween 80.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Details on results:

- No mortality after 48h in the experiment where FAT 36052/D is dissolved with acetone (8/8 are alive)
- No mortality after 48h in the experiment where FAT 36052/D is dissolved with Tween 80 (8/8 are alive)

Validity criteria fulfilled:

yes

Conclusions:

In conclusion, the result for FAT 36052/D dissolved in acetone and for FAT 36052/D dissolved in Tween 80 are:
*LC 0 >1000 mg/L: (FAT 36052/D dissolved in acetone and in Tween 80)
*LC 50 >1000 mg/L: (FAT 36052/D dissolved in acetone and in Tween 80)
*LC100 >1000 mg/L: (FAT 36052/D dissolved in acetone and in Tween 80)

Executive summary:

The acute fish toxicity study experiment was done with 8 fishes for each experiment (first FAT 36052/D is dissolved with Tween 80 and in the second, FAT 36052/D is dissolved with acetone). The temperature was 14 °C for both experience. During the test, the pH was between 8 and 8.1. At concentration 100 mg/L, 200 mg/L and 1000 mg/L of FAT 36052/D all fishes were alive at 24 hours and 48 hours for both conditions (dissolved with Tween 80 and with acetone).

 

In conclusion:

*LC 0 >1000 mg/L: (FAT 36052/D dissolved in acetone and in Tween 80)

*LC 50 >1000 mg/L: (FAT 36052/D dissolved in acetone and in Tween 80)

*LC100 >1000 mg/L: (FAT 36052/D dissolved in acetone and in Tween 80)

Description of key information

In all the three studies performed at concentration ranging from 3 mg/L to 1000 mg/L of FAT 36052/D there was no mortality noted at 24 hours and 48 hours for both conditions (dissolved with Tween 80 and with acetone).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

At Ciba Geigy AG acute fish toxicity testing was performed according to published methods, which were adapted and standardized for the specific needs of the company, well before Test Guidelines for these methods were established by the OECD beginning 1981. These methods were comparable to today’s standard testing procedure published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17^thJuly 1992. In all the three studies performed at concentration ranging from 3 mg/L to 1000 mg/L of FAT 36052/D there was no mortality noted at 24 hours and 48 hours for both conditions (dissolved with Tween 80 and with acetone).