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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06 Dec 2000 - 22 Jan 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one
EC Number:
259-701-7
EC Name:
17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one
Cas Number:
55542-27-3
Molecular formula:
C22H34O3
IUPAC Name:
17 beta-Hydroxy-17 alpha-(3-hydroxypropyl)-4-androsten-3-one
Details on test material:
- Name of test material (as cited in study report): Hydroxypropyltesto
- Analytical purity: 9l.2 %
- Lot/batch No.: 59012052

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC10
Effect conc.:
23.7 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Table 1: O2

 No.    Dissolved oxygen (mg/I)    
    0 hours    48 hours  
1 9,2 9,2

2

8,8 9,1

Table 2: pH

 No.       pH value     
    0 hours      48 hours
1 7,8    7,8
2 7,9    7,7

Table 3: °C

 Temperature °C
 No.    0 hours   48 hours
1 21,5 21,6
2 21,4 21,9

Applicant's summary and conclusion

Conclusions:
In the present study the saturated solution of the test item with a concentration of 23. 7mg/l
caused 10 per cent immobilisation of Daphnia. Since 10 per cent immobilisation is accepted for
the controls according to the OECD guideline, it is unclear, whether the observed
immobilisation is related to the test item.
Executive summary:

The acute toxicity of Hydroxypropyltesto to Daphnia magna was tested according to the OECD guideline No. 202, Part 1. Since the test item is almost insoluble in water the test was carried out with a saturated solution of the test item. For the toxicity test the Daphnia were exposed to a concentration of 23.7 mg/I (concentration of saturation) for 48 hours. The concentration of the test item in saturated solution was checked by DOC analysis (Dissolved Organic Carbon) at test start. The saturated solution of the test item caused 10 per cent immobilisation of Daphnia. Since 10 per cent immobilisation is accepted for the controls according to the OECD guideline, it is unclear, whether the observed immobilisation is related to the test item. According to the Annex IV of Directive 93/211EEC a classification of the test article is not required.