7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)

EC Number:

265-016-4

EC Name:

7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)

Cas Number:

64683-40-5

Molecular formula:

C22H17N5O7S2.2C6H15NO3

IUPAC Name:

7-amino-4-hydroxy-3-({4-[(4-sulfophenyl)diazenyl]phenyl}diazenyl)naphthalene-2-sulfonic acid - 2,2',2''-nitrilotriethanol (1:2)

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent from the Urban Water Treatment Plant in Manresa (Spain)
No further details on inoculum given in the study report.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
Solution 1:
KH2PO4 8.5 g/L H2O
K2HPO4 21.75 g/L H2O
Na2HPO4 2H2O 33.4g /L H2O
NH4Cl 0.5 g/L H2O
Solution 2: CaCl 2 2H2O 36.4 g/L H2O
Solution 3: MgSO 47H2O 22.5 g /L H2O
Solution 4: FeCl3 6H2O 0.25 g/L H2O
Add to 1000 mL of deionized water:
Solution 1 1 mL
Solution 2 1 mL
Solution 3 1 mL
Solution 4 1 mL
- Test temperature: 20 ± 2 ºC
- pH: not specified
- Aeration of dilution water: not specified
- Suspended solids concentration: not specified
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: Erlenmeyer flasks (100 ml) with a cap, fully filled
- Number of culture flasks/concentration: 2
- Measuring equipment: not specified
- Details of trap for CO2 and volatile organics if used: not specified
- Other:
Preparation of stock and test solutions: A stock solution having a concentration of 1 g/L of the material presented (ref: Red P / Paper 8BLP (batch: PAL-025777)) was prepared. Therefore, 0.1 g of the presented material has been dissolved in 100 mL of water in a 100 mL Class A volumetric flask by shaking, so we have a stock solution of 1000 mg / L. To prepare the solution to be tested (5 mg / L), 10 mL of stock solution was diluted in 2 liters of inoculated culture medium.
The test containers have been filled with the solution to overflow, so that no bubbles of air remain inside the bottle.

Concentrations per test: 1 + substance for reference + blank

SAMPLING
- Sampling frequency: at day 0, 3, 7, 10, 14, 21, 28 (Number of measurements: 7)
- Sampling method: not specified
- Sterility check if applicable: not specified

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: not specified
- Toxicity control: not specified

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

0 h

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

0.9

Sampling time:

3 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

2.3

Sampling time:

7 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

6

Sampling time:

10 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

11.1

Sampling time:

14 d

% Degradation 6

Parameter:

% degradation (O2 consumption)

Value:

13.7

Sampling time:

21 d

% Degradation 7

Key result

Parameter:

% degradation (O2 consumption)

Value:

18

Sampling time:

28 d

BOD5 / COD results

BOD5 / COD

Parameter:

COD

Value:

1.74 g O2/g test mat.

Results with reference substance:

Sodium acetate: 0,68 mg O2/mg

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The result obtained in this study indicates that the test item has a biodegradability of 18% at the initial concentration tested (5 mg / L) at 28 days of testing.

Executive summary:

The determination of the biodegradability has been carried out after 28 days for the test substance DR 254 TEA salt following the OECD Guideline 301D (closed bottle test) (1992) together with the Spanish Royal Decree 363/1995 (BOE No. 133 of June 1995).
Biodegradation is defined as the decomposition of an organic compound by the biological action of microorganisms, which results in the disappearance of the original molecular structure and the formation of smaller organic molecules.The results are expressed as the percentage of biodegradation after 28 days.

Starting from a known concentration of the test substance (5 mg / L), monitoring is carried out by measuring the oxygen consumption at intervals of time over a period of 28 days.