Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatephosphates

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

April 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 0345N/982432
- Stability in water: at least 96hours

Analytical monitoring:

no

Details on sampling:

- Concentrations: 0.1, 1, 1,0 100mg/L

Vehicle:

not specified

Details on test solutions:

prepared separately in water

Test organisms (species):

Daphnia magna

Details on test organisms:

not specified

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

n/a

Post exposure observation period:

not specified

Hardness:

not specified

Test temperature:

maintained within the optimum range for daphnids

pH:

maintained within the optimum range for daphnids

Dissolved oxygen:

maintained within the optimum range for daphnids

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

At the end of the test period of 48h, 90% immobilisation was observed in solutions prepared at 0.1 and 1 mg/L, while all daphnids exposed to concentrations of 10 and 100mg/L were immobilised

Results with reference substance (positive control):

none

Reported statistics and error estimates:

not specified

Validity criteria fulfilled:

yes

Conclusions:

0345N is acutely toxic to the freshwater crustacean daphnia magna at concentrations prepared at 0.1mg/L and higher. hence the 48h EC50 is below nominally 0.1mg/L

Description of key information

Two non-GLP screening test studies have been performed to assess the possible toxicity of the test

substance to fresh water crustaceans.

No analytical information is available to verify the concentration of the test substance in solution.

As the test substance is very poorly water soluble it is proposed to conduct a long term aquatic toxicity

test to fulfil this endpoint as indicated in the REACH guidance