Soybean meal

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Soybean meal

EC Number:

269-648-1

EC Name:

Soybean meal

Cas Number:

68308-36-1

IUPAC Name:

3-(4-hydroxyphenyl)-7-{[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-4H-chromen-4-one; 3-oxo-3-{[(2R,3S,4S,5R,6S)-3,4,5-trihydroxy-6-{[3-(4-hydroxyphenyl)-4-oxo-4H-chromen-7-yl]oxy}oxan-2-yl]methoxy}propanoic acid; 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one; 5-hydroxy-3-(4-hydroxyphenyl)-7-{[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-4H-chromen-4-one; 7-hydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one

Sampling and analysis

Analytical monitoring:

yes

Remarks:

TOC

Test solutions

Vehicle:

yes

Remarks:

water

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

other: deionised water

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

Water temperature: 19.2 to 21.0 °C

pH:

7.5 to 8.5

Dissolved oxygen:

8.4 to 9.7 mg/L

Nominal and measured concentrations:

Nominal concentrations: 100, 50, 25, 12.5 and 6.25 mg test item/L.
All reported results refer to nominal values since the measured concentrations of the carbon content of the test item were not quantifiable because of the poor solubility of the test item.

Details on test conditions:

Photoperiod: 16 h light - 8 h dark
Light intensity: 580 to 800 lux;

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

48h EC50 = 1.02 mg/L
48h NOEC = 0.25 mg/L
48h LOEC = 0.5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item Soya flour to Daphnia magna was assessed in a semi-static concentration-response test.
The 48-hour NOEC was determined to be ≥ 100 mg test item/L. The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was calculated to be > 100 mg test item/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part.
All reported results refer to nominal values since the measured concentrations of the carbon content of the test item were not quantifiable because of the poor solubility of the test item.