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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Soybean meal
EC Number:
269-648-1
EC Name:
Soybean meal
Cas Number:
68308-36-1
IUPAC Name:
3-(4-hydroxyphenyl)-7-{[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-4H-chromen-4-one; 3-oxo-3-{[(2R,3S,4S,5R,6S)-3,4,5-trihydroxy-6-{[3-(4-hydroxyphenyl)-4-oxo-4H-chromen-7-yl]oxy}oxan-2-yl]methoxy}propanoic acid; 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one; 5-hydroxy-3-(4-hydroxyphenyl)-7-{[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-4H-chromen-4-one; 7-hydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one

Sampling and analysis

Analytical monitoring:
yes
Remarks:
TOC

Test solutions

Vehicle:
yes
Remarks:
water

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
other: deionised water
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
0.24 mmol/L (= 24 mg/L) as CaCO3
Test temperature:
Water temperature: 22.7 to 23.1 °C
pH:
at test start: 7.8 to 8.0
at the end of the test: 8.8 to 10.2
Dissolved oxygen:
No information
Nominal and measured concentrations:
Nominal: 100, 33.3, 11.1, 3.70 and 1.23 mg test item/L
All reported results refer to nominal values since most of the measured concentrations of the carbon content of the test item were not quantifiable because of the poor solubility of the test item.
Details on test conditions:
continuous illumination; mean light intensity: 7655 lux (7250 to 7990 lux)
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
65.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
11.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
33.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
33.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Results with reference substance (positive control):
Results on reference item based on growth rate:
72h EC50 = 1.02 mg/L
72h NOEC = 0.094 mg/L
72h LOEC = 0.21 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The influence of Soya flour on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static concentration-response test.
The 72-hour EyC50 was calculated to be 65.4 mg test item/L, and the 72-hour ErC50 value was calculated to be > 100 mg test item/L.
The 72-hour NOEyC was determined to be 33.3 mg test item/L and the associated 72-hour LOEyC was 100 mg test item/L. The 72-hour NOErC was determined to be 11.1 mg test item/L and the associated 72-hour LOErC was 33.3 mg test item/L.
All reported results refer to nominal values since most of the measured concentrations of the carbon content of the test item were not quantifiable because of the poor solubility of the test item.