Tris[2-[[2,4,8,10-tetra-tert-butyldibenzo[d,f][1,3,2]dioxaphosphepin-6-yl]oxy]ethyl]amine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tris[2-[[2,4,8,10-tetra-tert-butyldibenzo[d,f][1,3,2]dioxaphosphepin-6-yl]oxy]ethyl]amine

EC Number:

279-459-6

EC Name:

Tris[2-[[2,4,8,10-tetra-tert-butyldibenzo[d,f][1,3,2]dioxaphosphepin-6-yl]oxy]ethyl]amine

Cas Number:

80410-33-9

Molecular formula:

C90H132NO9P3

IUPAC Name:

2-[(2,4,8,10-tetra-tert-butyldibenzo[d,f][1,3,2]dioxaphosphepin-6-yl)oxy]-N,N-bis{2-[(2,4,8,10-tetra-tert-butyldibenzo[d,f][1,3,2]dioxaphosphepin-6-yl)oxy]ethyl}ethanamine

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The standard activated sludge purchased from Chemical Safety Center, Chemicals Inspection & Testing Institute, on April 20, 1995, was used.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: according to JIS 0102-21(1)
- Test temperature: 25.0-26°C
- pH: 6.99 - 7.27
- Suspended solids concentration: 30mg/L
- Test soliution: 300mL


TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption meter, Okura Electric Co, Ltd " Coulometer OM-2000"
- Number of culture flasks/concentration: 3

SAMPLING
- Sampling frequency: direct measuremet of test substance concentration: after 28 days
- Sampling frequency: direct measuremet of test substance concentration: after 7, 14, 21, 28 days

CONTROL AND BLANK SYSTEM
- Inoculum blank (basic respiration chamber: mineral medium + inoculum): 1 vessel
- water stability chamber (test substance in mineral medium): 1 vessel


Observations: Color of the solution, degree of growth of the standard activated sludge, dissolution status of the test substance, pH

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

0.2

Sampling time:

28 d

% Degradation 2

Key result

Parameter:

% degradation (test mat. analysis)

Value:

1.1

Sampling time:

28 d

Details on results:

Degradation based on oxygen consiumption: 0.2, -1.9, 0.0%
Degradation based on measurement of residual test substance concentration: 0.7, 1.1, 0.6%

Validity criteria:
- inoculum blank oxygen uptake (28d) 12.8mg/0.3L- validity criterion fulfilled (< 60mg/L)
- pH 6.99 - 7.27 - validity criterion fullfilled (6-8.5)
- degradation of aniline calculated from the oxygen consumption exceeds after 7 days 57.5 % and after 14 days 68.5% - validity criterion fullfilled (> 40% and > 65%, respectively)
- replicate values of the removal of the test substance at the plateau or at the end of the test < 20% - not applicable as no biodegradation observed

BOD5 / COD results

Results with reference substance:

73.8% degradation after 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed