4-[2-[4-[benzylmethyl(ethyl)amino]phenyl]vinyl]-1-(2-hydroxyethyl)pyridinium acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 January 2018 to 07 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Analytical samples were taken from all test item concentrations and control at test start and 24 hours (fresh) after 48 hours from aged solutions. Samples (50 mL) were stored deep-frozen (≤ - 18 °C) until analysis. In the analytical laboratory, the samples were thawed to ambient temperature, mixed with 50 mL acetonitrile and shaken well using a flatbed shaker for 15 min. If necessary, the samples were further diluted with acetonitrile/test medium (1:1, v/v) prior to analysis by HPLC-MS/MS.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Clone V
- Source: in house originating from Federal Environment Agency in Berlin/Germany.
- Age of parental stock: < 24 h
- Feeding during test: none

CULTURE CONDITIONS
- Conditions: single culture (1 daphnid per 100 mL) in Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3)
- Type and amount of food: single cell green algae (Desmodesmus subsp.)
- Feeding frequency: at least 3 times per week

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

232 mg/L as CaCO3

Test temperature:

18.9- 19.9°C

pH:

7.45-7.92

Dissolved oxygen:

8.8-9.2 mg/L

Salinity:

NA

Nominal and measured concentrations:

Nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L
Measured: 85-103% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL all glass covered with glass plate (fill volume 50 mL)
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ELENDT M4 medium according to the guidance

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light/ 8 dark
- Light intensity: no data

EFFECT PARAMETERS MEASURED : immobility at 0, 24 and 48 hours

RANGE-FINDING STUDY
- Test concentrations: Semi-static at 0, 0.01, 0.1, 1 and 10 mg/L in a closed system
- Results used to determine the conditions for the definitive study: immobility after 24 and 48 h: 5, 0, 0, 100 and 100%

Reference substance (positive control):

yes

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.492 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 potassium dichromate 1-2 mg/L

Reported statistics and error estimates:

EC50 with Probit analysis using linear max likelihood regression

Results of the main test, 24 h values

	Nominal test item concentration [mg/L]
	Control	0.0625	0.125	0.250	0.500	1.00
	Immobilised daphnids after 24 h
Group 1	1	0	0	0	0	1
Group 2	0	0	0	0	0	1
Group 3	0	0	0	0	0	1
Group 4	0	0	0	0	0	0
Σ	1	0	0	0	0	3
%	5	0	0	0	0	15

 Results of the main test, 48 h values

	Nominal test item concentration [mg/L]
	Control	0.0625	0.125	0.250	0.500	1.00
	Immobilised daphnids after 48 h
Group 1	2*	0	0	0	5	5
Group 2	0	0	0	0	3	5
Group 3	0	0	0	0	2	5
Group 4	0	0	0	0	2	4
Σ	1	0	0	0	12	19
%	5	0	0	0	60	95

* One daphnid died accidentally and was therefore excluded from evaluation

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the substance is 0.492 mg/L

Executive summary:

In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L ; measured: 85-103% of nominal) were exposed for 48 hours. Immobility was observed at concentrations of 0.500 mg/L and higher. The EC50 of the substance is 0.492 mg/L (measured).

Description of key information

In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L ; measured: 85-103% of nominal) were exposed for 48 hours. Immobility was observed at concentrations of 0.500 mg/L and higher. The EC50 of the substance is 0.492 mg/L (measured).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.492 mg/L