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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988-09-06 to 1988-09-07
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard methods with acceptable restrictions: Many details of standard procedure not included in report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 11
Deviations:
not specified
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2,4(or 2,4,4)-trimethylhexanedinitrile
EC Number:
283-810-9
EC Name:
2,2,4(or 2,4,4)-trimethylhexanedinitrile
Cas Number:
84713-17-7
Molecular formula:
C9H14N2
IUPAC Name:
2,2,4-trimethylhexanedinitrile; 2,4,4-trimethylhexanedinitrile
Details on test material:
2,2,4 (or 2,4,4)-Trimethylhexanedinitrile of Hüls AG, commercial purity (not quantified)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration: 1 g/l, no solvent or vehicle

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Nominal and measured concentrations:
100; 180; 300; 550; 900 mg/l (nominal)
Details on test conditions:
Number of replicates, individuals per replicate: 4 replicates with 5 individuals each
TEST PARAMETER: immobilisation
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC10
Effect conc.:
157.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
401.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 326.6-493.7 mg/L
Duration:
24 h
Dose descriptor:
other: EC90
Effect conc.:
1 025.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Effect data (Immobilisation): No. of immobile daphnia
  Control:    0 (24 h) 
   100 mg/l:  1 (24 h)  
   180 mg/l:  3 (24 h) 
   300 mg/l:  6 (24 h) 
   550 mg/l: 11 (24 h) 
   900 mg/l: 20 (24 h)  

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 (24 hours) was calculated as 401.6 mg/L indicating that the test substance does not pose a hazard to aquatic macroinvertebrates.
Executive summary:

The acute effects of 2,2,4(or 2,4,4)-trimethylhexanedinitrile on Daphnia magna were investigated by Hüls AG (1988) over a period of 24 hours according to national accepted guidance documents. Five concentrations ranging from 100.0 to 900.0 mg/L were tested. The following enpoint was derived:

EC50 (24 hours): 401 mg/L (CL: 326.6 - 493.7 mg/L)

The study was assessed as "reliable with restrictions".