Registration Dossier
Registration Dossier
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EC number: 284-515-8 | CAS number: 84929-31-7 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Citrus limonum, Rutaceae.
Endpoint summary
Administrative data
Description of key information
Skin irritation: irritating
Eye irritation: not irritating
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Additional information
Skin irritation potential of lemon oil was determined in an acute dermal toxicity limit test. Lemon oil was applied to abraded and unabraded skin of six male/female rabbits. A dose of 5 ml/kg of liquid Lemon oil was applied and occluded for 24 hours. After removal of the patches, local dermal reactions were scored according to the method of Draize. Toxic effects, dermal reactions, and mortality were recorded daily for 14 days. Erythema, edema, dicoloration, scaling and eschar formation were observed. As no detailed scoring data was available, it was not possible to classify lemon oil according to the EU classification criteria outlined in 67/548/EEC and 1272/2008. However, the available scores at 24 hrs indicate lemon oil should be considered irritating to the skin.
To determine if lemon oil produces any eye irritation, lemon oil was instilled into the eyes of three normal, healthy, albino rabbits. Irritation was scored according to the Draize scale for scoring ocular lesions every 24 hours for 4 days and then again on the 7thday.
Lemon oil did not produce any corneal opacity or iris congestion, but an intense conjunctival irritation did occur involving chemosis and discharge. On the 7thday of observation the treated eyes were normal. Based on the irritation scores and according to the EU classification criteria outlined in 67/548/EEC and 1272/2008, Lemon oil does not have to be classified as irritating to eye.
Effects on skin irritation/corrosion: irritating
Justification for classification or non-classification
Based on the available information, lemon oil has been shown to have irritating properties when applied to the skin. Although the available information cannot be used for classification according to the criteria outlined in Annex VI of 67/548/EEC and Annex I of 1272/2008/EC (CLP/EU-GHS), lemon oil should be considered to have skin irritating properties.
The results of the primary eye irritation test indicate that the substance lemon oil does not need to be classified as eye irritant according to the criteria outlined in Annex VI of 67/548/EEC and Annex I of 1272/2008/EC (CLP/EU-GHS).
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