Bis(C12-C13)alkyl-2-hydroxybutandioate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

5.4. 1993 - 7.4.1993

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

-Name of test material (as cited in study report): Cosmacol EMI, Butanedioic acid, hydroxy-, di C12-C13-alkyl esters
- Molecular weight (if other than submission substance): 470 - 498
- Substance type: pure active substance
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: NOTHING

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Water institute Research - Brugherio (MI)
- Age at study initiation: 6 - 24 hours
- Weight at study initiation (mean and range, SD): not mentioned
- Food type: Selenastrium Capricornutum and yeast, Saccharomyces cerevisiae
- Frequency: daily

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

150 - 180 mg CaCO3/L

Test temperature:

20 +-12 ° C

Dissolved oxygen:

> 3 mg/L

Nominal and measured concentrations:

It was not possible, with the analytical technology available to measure the concentration of the substance in the solution.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass becker
- Aeration: porous stones
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours of artificial light and 12 hours darkness
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L dispersion concentration, 1 mg/L water solubility

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

not specified

Conc. based on:

other: dispersion concentration

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

not specified

Conc. based on:

other: dispersion concentration

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

not specified

Conc. based on:

other: dispersion concentration

Basis for effect:

mobility

Details on results:

No immobilization phenomena were observed in animals treated with COSMACOL EMI and in the controls. The pH did not change more than 0.2 units during the test. During the study the oxygen concentration remained above 3 mg/L The temperature remained constant at 21 °C.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

25 Daphniae were used, of which 20 were treated with the test material COSMACOL EMI at a concentration exceeding the solubility of the substance. 5 were used as the control. The Daphniae were kept under observation for a period of 48 hours. At the end of the observations period, no immobilisation phenomena were seen in the group treated or in the control group (EC50 > 100 mg/L).