Aluminium tridiethylphosphinate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Details on sampling:

nominal concentrations: 3.2, 5.8, 10, 18, 32, 58, 100, 180 mg/l

Vehicle:

yes

Details on test solutions:

The samples with dosage levels of 5.8 to 180 mg/l were treated first time for 15 min with ultra sound. Then they were broken down by adding 0.1 mHCl to 5.0 ml sample in a 10 ml sample; heated to 100°C for 45 min. Addition NaOH to get pH-value of approx. 5.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Strain: CHODAT SAG 86.81

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test temperature:

23 +/-2°C

pH:

8.1

Details on test conditions:

- Initial cells density: 0.8 - 1.1 E4 cells/ml
- Control end cells density:

- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):3


GROWTH MEDIUM
- Standard medium used: yes


- Light intensity and quality: 35-70 [µE/m2xs] (Fluorescent tube, Radium NL 58W/31, Spectralux Warmton).



Chlorophyll measurement: Impulsfluorometer

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Validity criteria fulfilled:

yes

Conclusions:

The growth inhibition of the test substance to the unicellular algal specie Scenedesmus subspicatus was determined according to EEC-Guideline 92/69 N. L383 C.3. from January 19 to 22, 1998. A static test was conducted with the nominal concentrations.
The test item caused a pH related effect on both biomass inhibition and rate inhibition. No inhibition was found at the pH adjusted 180 mg/L level.
 
ErC50 > 180 mg/L
NOEC = 180 mg/L

Executive summary:

The growth inhibition of the test substance to the unicellular algal specie Scenedesmus subspicatus was determined according to EEC-Guideline 92/69 N. L383 C.3. from January 19 to 22, 1998. A static test was conducted with the nominal concentrations.

3.2 – 5.8 – 10 – 18 -32 – 58 -100 -180 mg/L

Selected based on a preliminary static test.

The 180 mg/L dosage with pH adjustment (pH 8.0 +/- 0.1) was additionally tested.

Exposure duration of the test was 72 hours. The initial cell density was 0.8 – 1.1 x 10⁴cells/mL. No vehicles were used to dissolve the test substance. The dispersion of the test substance in water.

The concentrations of the active ingredient (determined as Al3+) were determined in all concentration levels at test start and test end. The recovery rates at the beginning and end of the test were < 80 % at the lowest dosage of 3.3 mg/L, whereas recovery rates of all other dosage levels were > 80 %. All effect concentrations are based on real concentrations of the test substance.

 

The test item caused a pH related effect on both biomass inhibition and rate inhibition. No inhibition was found at the pH adjusted 180 mg/L level.

 

ErC50 > 180 mg/L

NOEC = 180 mg/L

 

Description of key information

The growth inhibition of the test substance to the unicellular algal specie Scenedesmus subspicatus was determined according to EEC-Guideline 92/69 N. L383 C.3. from January 19 to 22, 1998. A static test was conducted with the nominal concentrations.

The test item caused a pH related effect on both biomass inhibition and rate inhibition. No inhibition was found at the pH adjusted 180 mg/L level.

 

ErC50 > 180 mg/L

NOEC = 180 mg/L

Key value for chemical safety assessment

EC10 or NOEC for freshwater algae:

180 mg/L