Ethanone, 2-chloro-1-(1-chlorocyclopropyl)-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not applicable

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

Gas Chromatography (GC)

Details on sampling:

Sampling schedule of chemical analysis
- Control: at O and 48 hours
- Test concentrations: at 0 and 48 hours at 0 and 48 hours

Vehicle:

no

Details on test solutions:

To produce the highest test concentration (1.2 mg/L) the test substance was added daily to 1 litre of dilution water, then treated with an ultra turrax for 60 seconds and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration. The test concentrations 0.125, 0.25, and 0.5 mg/L were prepared daily by diluting a stock solution (0.5 mg/L) and treated in the same way as the test concentration 1 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

Name: Daphnia magna STRAUS, parthenogenetic females
Source: Strain ofBundesgesundheitsamt Berlin
Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus CHODAT) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semiquantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

15.0 °dH (= 267.8 mg/L CaCO3)

Test temperature:

19.8 - 19.9 °C

pH:

7.8 - 7.9

Dissolved oxygen:

7.6 - 8.6 mg/L (concerning the highest test concentration)

Salinity:

n.a.

Conductivity:

No data

Nominal and measured concentrations:

- Test concentrations (nominally): 0.125, 0.25, 0.5 and 1 mg/L
- Test concentrations (measured): in the range between 62.4 and 92% of nominal values in the freshly prepared medium and between 20 and 52% of nominal values after 24 hours of exposure

Details on test conditions:

Exposure conditions:
- Test vessels: Karlsruher Flaschen holding 10 neonates in 250-300 mL of test medium
- Experimental design: 4 test concentrations plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period; closed bottle conditions
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light: 8 h dark
- Temperature: mean +/- 1 °C (in the temperature range 18 - 22 °C)
- Aeration: none
- Method of administration: stock solution
- Medium renewal: none
- Criteria of effects: The criterion of adverse effects used in this study was the substance-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.31 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.62 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.155 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The results are expressed in terms of mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from 45.6- 52% of nominal values at 48 hours, respectively.

Validity criteria fulfilled:

yes

Conclusions:

After 48 hours of exposure both an EC0 of 0.155 mg/L and an LC100 of 0.62 mg/L was measured. The geometric mean of the two values corresponding to the EC 50 value is 0.31 mg/L. The results are expressed in terms mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from 45.6- 52% of nominal values at 48 hours, respectively.

Executive summary:

A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions in a closed bottle system. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'. The Daphnia were exposed to a range of concentrations, nominally 0.125, 0.25, 0.5 and 1 mg/L of test item dissolved in water. After 48 hours of exposure both an EC0 of 0.155 mg/L and an LC100 of 0.62 mg/L was measured. The geometric mean of the two values corresponding to the EC50 value is 0.31 mg/L. The results are expressed in terms of mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from 45.6- 52% of nominal values at 48 hours, respectively. The hardness of the dilution water used was 15.0 °dH. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute daphnia study.

Description of key information

A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions in a closed bottle system. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'. The Daphnia were exposed to a range of concentrations, nominally 0.125, 0.25, 0.5 and 1 mg/L of test item dissolved in water. After 48 hours of exposure both an EC0 of 0.155 mg/L and an LC100 of 0.62 mg/L was measured. The geometric mean of the two values corresponding to the EC50 value is 0.31 mg/L. The results are expressed in terms of mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from45.6- 52% of nominal values at 48 hours, respectively. The hardness of the dilution water used was 15.0 °dH.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.31 mg/L