Registration Dossier
Registration Dossier
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EC number: 447-010-5 | CAS number: 670241-72-2 ISONONYLBENZOAT
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The NOEC (21d) is > = 0.078 mg/L, which constitutes the limit of solubility of the test item under the test condition of this study. No mortality and no adverse effects on reproduction were observed. On the contrary, the reproduction rate of the exposed daphnids showed a significant increase compared to the control values.
Key value for chemical safety assessment
Additional information
The Daphnia magna STRAUS Reproduction Test (21d) of Isononyl benzoate was conduced as a limit test with the saturated solution at the range of water solubility according to OECD 211 (1998) and Directive 2001/59/EC Method C.20 (2001). The saturated solution is the maximum dissolved concentration of the test item that can be achieved under the conditions of the the slow stirring method in the test medium, acc. to OECD Series, No. 23 (2000)6.
The Test system was Dapnia magna STRAUS (Clone 5). 10 test organisms, individually held were used for the limit concentration and the control. At test start the daphnids were 2 to 24 hours old. The test method was semi-static. Test solutions wer renewed daily. Aim of the test was to assess effects on the reproduction rate and other test item-related effects on reproduction parameters such as occurrence of aborted eggs, stillborn juveniles, males and ephippia, time of release of first brood, number of broods, adult mortality and the intrinsic rate of natural increase. The test concentration was verified analytically by HPLC. The NOEC (21d) > = 0.078 mg/L, which constitutes the limit of solubility of the test item under the test condition of this study. No mortality and no adverse effects on reproduction were observed. On the contrary, the reproduction rate of the exposed daphnids showed a significant increase compared to the control values.
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