2-(chloromethyl)-4-methylquinazoline

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-01-29 to 2007-03-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

1996

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: samples from the test media of the test concentration and the control at the start and at the end of the test
- Sampling method: samples were taken from the approximate centre of the aquaria without mixing of the test media
- Sample storage conditions before analysis: All samples were analysed immediately after sampling. The samples are kept stored in deep-freezer up to the date of the final report.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A stock solution with a concentration of 400 mg/L was prepared with test item and ISO-medium. At this concentration the test item formed a stable dispersion (suspension) in the medium used. The work solution was prepared by diluting the stock solution by a 400-fold dilution (2.5 ml ad 1000 ml ISO-medium).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna (Straus), Female
- Source: National Institute of Public Health, Hungary
- Age at study initiation (mean and range, SD): Less than 24 hours old
- Method of breeding: They were bred in the laboratories of LAB International Hungary under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.
- Feeding during test: None

ACCLIMATION
- Acclimation period: > 6 hours
- Acclimation conditions (same as test or not): same as test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

168.0 mg CaCO3/L (Reconstituted Water)

Test temperature:

20.7 – 21.3 °C

pH:

6.87 - 7.41

Dissolved oxygen:

6.2 - 6.9 mg/L

Nominal and measured concentrations:

- Nominal: 0.063; 0.125; 0.250; 0.50 and 1.00 mg test item/L
- Measured: 0.046; 0.108; 0.210; 0.375; and 0.780 mg test item/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers
- Material, fill volume: 40 mL test medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The ISO medium (according to OECD 202) was prepared by adding 25 ml each of the following stock solutions to deionised water.
CaCl2 x 2 H2O 11.76 g/L; Batch No.: 828771, Supplier: REANAL
MgSO4 x 7H2O 4.93 g/L; Batch No.: A677186 601, Supplier: MERCK
NaHCO3 2.59 g/L; Batch No.: 829880, Supplier: REANAL
KCl 0.23 g/L; Batch No.: KBM5010801, Supplier: REANAL


OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility, pH, Dissolved Oxygen and Water Temperature

TEST CONDITIONS:
- Observation time: 24 and 48 hours after start of the test

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.57 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks on result:

other: 95% CL = 0.46 - 0.71

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.18 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL = 0.16 – 0.21

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.78 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: nominal: 1.00

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

< 0.046 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: nominal: 0.063

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

< 0.046 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: nominal: 0.063

Analytical results:

The analytically determined mean test item concentrations in the test media varied in the range of 56 % to 114 % of the nominal value.

Concentration (mg/L)				%-loss in the measured test item concentration during the test
nominal	measured
	start	end	average
0.063	0.055	0.036	0.046	34.55
0.125	0.142	0.073	0.108	48.59
0.250	0.28	0.14	0.210	50.00
0.500	0.46	0.29	0.375	36.96
1.000	0.92	0.64	0.780	30.43

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 value of 0.18 mg/L at 21°C for Daphnia magna was determined for the test item under the conditions of the test. It was determined in a reliable study conducted according to appropriate standard test methods (EU Method C.2 (Acute Toxicity for Daphnia)/OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test)).

Description of key information

A 48 -h EC50 value of 0.18 mg/L for Daphnia magna was determined for the test item under the conditions of the test. It was determined in a reliable study conducted according to an appropriate standard test method (OECD 202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.18 mg/L